FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb3_1_10.34100000009628.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb3_1_10.34100000009628.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences334909
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA18140.5416396692832979No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17210.5138709321039446No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13300.3971228005219328No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA10530.3144137661275152No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9630.28754079466362503No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9490.283360554658131No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7860.2346906174513077No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA7720.23051037744581362No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6350.18960374310633635No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA6210.18542350310084232No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGCG4970.14839852019503808No Hit
CTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGTC4880.14571122304864903No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA4740.141530983043155No Hit
ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA4150.12391425730571587No Hit
GTATCAACGCAGAGTACGGGTTTCCCCCGACTCTTAGGATCGACTAACCCA3830.11435942300744381No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATG3640.10868624014284477No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA3550.10599894299645575No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3510.10480458870917174No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGCC3430.10241588013460373No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGACCG250.002105392735.9985733
CTGGCGT250.002105392735.9985710
TCCATGC300.00514311429.9988048
GTTCGTT406.158384E-428.1238817
CACAGAT903.929017E-1027.4989057
AATGCAA1004.5474735E-1126.99892627
TAGATGT608.1714425E-626.2489554
GCAAAGC957.366907E-1026.05159630
TAGGATA1600.025.3114914
AGGATAG1700.025.1460595
GTATAAT450.001212280825.0438731
ATAATGG450.001225024324.9990043
ATCTATG551.3492176E-424.588531
GTATAGA551.3492176E-424.588531
ACACAGT551.3657463E-424.5444776
TACACAG1101.5097612E-1024.5444775
AGATCGG952.1049345E-823.6832710
TGCAAAG952.1049345E-823.6832729
GTACAGG1405.456968E-1222.5394861
GCGCGCT702.6964406E-522.49910511
Produced by FastQC (version 0.11.2)