FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_4_95.3410000000b30a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_4_95.3410000000b30a.fastq.gz
File typeConventional base calls
EncodingIllumina 1.3
Total Sequences2
Sequences flagged as poor quality0
Sequence length51
%GC62

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC150.0RNA PCR Primer, Index 23 (95% over 21bp)
GGGTGACGGTGGGAGAGGATTCGCCGGCTGCACCGGCCAGCTCCTCCAGCG150.0No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)