FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_4_94.3410000000b2fc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_4_94.3410000000b2fc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38
Sequences flagged as poor quality0
Sequence length51
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCACGTGGAATGCTAGGGCCGCGATAGCCAGCAGTTGCAATCGGTGACT12.631578947368421No Hit
AGGCTGCGGCGCCAGCCCATCCTGGCCGATGCCGCCGCACGGCTGCAGTAC12.631578947368421No Hit
AGTTTTACATGAGAAGATTATAGGTGCTGTTTATGCTAGTTGGGAATCTTC12.631578947368421No Hit
CTCATATCCATCAAGGAAGATGTATTGACATCCTCCACAAATCCAAGGAGG12.631578947368421No Hit
CTCCCGGTTTGCCTCGTAAATAAATTTACGATACCTGTCTTATGACTGTCT12.631578947368421No Hit
ATAACAAAGAAGAGGAACACAACAAGGCTGAGAAAGCTGAGAAGAAGGAGA12.631578947368421No Hit
CCATAAATAGTTGTGAACAAGAATGTCTGGCTTGTACCCTGATATTTCCTC12.631578947368421No Hit
CCGTGATAGCTGGTTCTCCCCGAAATGCATTTAGGTGCAGCGTCGCGTGTT12.631578947368421No Hit
TCCTTATTCCTCATCGTCGTCATCATCATCTTCCTTCTTCAGACGGGTCTC12.631578947368421No Hit
TACAGATAGATTGTAACCGCTTTGTATGTTAACATACAAAAGTGAGTGATA12.631578947368421No Hit
AGCCTACACAGGGTTACCACTTTCATTAACAGCACCCTGTATCCTGAACCT12.631578947368421No Hit
GCAATAAACCAGAGAGACGAATCAAAAGGAAAAAATGTTCTGTCTCCTATA12.631578947368421No Hit
GGTTGGCGGCCACCATCGCATAGCTCGGGTTGGGCAGGCCCTGGCGCTGCG12.631578947368421No Hit
TCCGAATACAGGCCGTTCTCGTTTGTACAGACAGACTTTTGGGGTGCTAAG12.631578947368421No Hit
ATCCTAAACATCGAATAAATGGATAACATGAGAGGAAGAAACCTGTCTCTT12.631578947368421No Hit
GGAGCAAAATCGACCAGCTTAAACAATTGGTTAACCGGTGTTTACAATTAC12.631578947368421No Hit
GTATCAACGCAGAGTACGGGAGTGGTATCCTGTCTCTTATACACATCTCCG12.631578947368421No Hit
ATGTAGTAGTATCACAAACAAGAAACTCAGAGAAAACTGGGAGAATTTCTG12.631578947368421No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC12.631578947368421No Hit
TTCCCACAGACACTAGATTGGCTTCACAACTATCTTCCTGTCTCTTATACA12.631578947368421No Hit
CTACCACCAAGATCTGCACCGACGGCCGCTCCGCCCGGGCTCGCGCCCTAG12.631578947368421No Hit
CATCAATATTACAACATTGCGAATGAAATTCCAAAGAAGTAGGTACCTGTC12.631578947368421No Hit
CATTATCATTCACTTGTTGTATTGTGAATCTAAACCGCTGTATGTGAAGAA12.631578947368421No Hit
CAAGAAAGCACCTATGAAAAACAACAACAAGAAACCTGTATCCATAATCCC12.631578947368421No Hit
ATGATTGACCATAACACGATGTGATTACAATCAATTACAAAGCCAAATTAA12.631578947368421No Hit
GACTTGTCGTTGCGCGTCAGATCGGGCGAGATGATCTCCCACGACCTGTCT12.631578947368421No Hit
CTAGTATCCGATTTTCCAAGTAAATTTCCATGGATGAAGTTCTATATCCAC12.631578947368421No Hit
ATAAATCTATGAGTCTTCAAGCTCCATTCATTGTTTGGCTGTGGGTGCCTG12.631578947368421No Hit
TTGCACACGAGGCTCGTGAATCTAGAGCACGTGCAAACAAGCTGTCTCTTA12.631578947368421No Hit
CAACAAATTTAGCGGTTGTATCCCTAGAAGTATTGGAAACATGAAGAATCT12.631578947368421No Hit
GTTGAAGAGAGCTCTAACAGGCCTTGGAGGACCGAACCCACGTATGTGGCA12.631578947368421No Hit
GAACTTCCACATCAACGTGTTGGGAGCCAAGAACATGACCATGAATAACAT12.631578947368421No Hit
GAAGAAGTAAGGATCACCGGCGTTCACGATCGCCACCGTAGAGACGCTGTC12.631578947368421No Hit
CTAAGCAATGCAAAAACCAACTTTTTTTTTTTTTTCAGTCTTAGGCTGGTC12.631578947368421No Hit
GGCTTCCCAAGTGCTGATGTCCCAAACAGGAAGCAAGCCATTGTCTTTTTC12.631578947368421No Hit
GAATTCCAATGTTGCAGTTAGCTTGACCTCCATTAGCCTTACACTTTGGAG12.631578947368421No Hit
GAGGCGGGCGAGGGCCCGGAGCCCGCCGACACCGCGGCCGATGACCTGCAG12.631578947368421No Hit
ACAACAACAGCAGGATTCCTATAATCCAAATGCTAAATATCAAGGCGGTCA12.631578947368421No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)