FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_4_93.3410000000b2ef.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_4_93.3410000000b2ef.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC23.076923076923077RNA PCR Primer, Index 23 (95% over 21bp)
ATAGATTCTTTCTTCACCATAGATCTAATCTAAATCTGTCTCTTATACACA11.5384615384615385No Hit
CTCATGACAAACTCATAGAACTCTTCGAAAACTGGATCTCAAATTTTGAGA11.5384615384615385No Hit
ATTAGAAGGTGTTATAGCTGTAGTTGACTAAAGGATTCGGTTATAAAGCTG11.5384615384615385No Hit
CTTTTACAGTCTACACAAAAGACGCTATACAAAACCTGTCTCTTATACACA11.5384615384615385No Hit
CACTCGTCCAGCAGGCCAGCTTCCTTTAATGCACTCTTATACTCCTGTCTC11.5384615384615385No Hit
GCCTATCAACGTCCTGGTCTCGAACGACCCTTCAAGGAGGTCAAGCCTCCA11.5384615384615385No Hit
CCGCTGGGCACGGCGGCCAACGACTTGGTCAATGCCAGGGACGCTCTGCAT11.5384615384615385No Hit
CCTTCACTACGGGGAAGCGGCGTTAGTATCGTGGGCAAGACGGATGAAGAT11.5384615384615385No Hit
GCGATCAACTGCCGCATCGCATCGAGCAGATGCGGCTCCAGGTGCGCCCCC11.5384615384615385No Hit
TCATAGTTCCAACTTTAAAATTTTGTAAACTTGCACTTTGCTTTGTGTTGT11.5384615384615385No Hit
GCAGCAAAGTAAAACTGAGGGCGGCAAGGCCTAGCATGACATTGTTGTACT11.5384615384615385No Hit
GATGAGGAATTGTTACTATGTAGTCACAGATGGAGGAAGGATGAGGAATTG11.5384615384615385No Hit
GTCTTCAGATACAGTTATTAGCAACTTCAGAAAAGCATCTGTCTCTTATAC11.5384615384615385No Hit
GCATATCATCCTGACAAAGCTGAGTATACAACGATCTTAACTCCTGTCTCT11.5384615384615385No Hit
ATACAATCCCAGATTCTCTTCGATGAGTACCTTAGAATCAGATTCTGTCTC11.5384615384615385No Hit
GATCTCACCATTTACAAGAATGCTTGTTTTTGAGTATGCATCAAACGGAAC11.5384615384615385No Hit
AATCCTTACGTCATCAATCTCATGGATGAACTTACGCTCAAGGGTATTACC11.5384615384615385No Hit
TCATAATGGAATTCGCGGAGAATTTGGTGTTAAGGTTAATGGAGAATCCAG11.5384615384615385No Hit
GTTATACAGAGTACGAGAGAGAAATAGAAGACGAAGAAGAAGAAGGAGATT11.5384615384615385No Hit
GTCATAACCGTACCCTTAAATTGTGTTTTGTTTCATTGGTAGATAATCTGT11.5384615384615385No Hit
GCTTAAGATGAAGCATGTTAAGGTATTGGTTAAAGGGTGGACTTGGATCTG11.5384615384615385No Hit
GTATGATGCTTTGAGTGAGTCTCCTTTGTCTCCCAATATTATGGAGCTGTC11.5384615384615385No Hit
AGCCGCCAGTGAACGCCACCCGGCCATCGATGATCAGCAACTTGCGATGGT11.5384615384615385No Hit
CTTATCAAAGATCCGATACTGGTGGTCGAGCTCGGCCTGGCCATCTGAACT11.5384615384615385No Hit
GGATAAGGATGAACTGGTTACGTCACCTTGTTTGAAAGAACGTAACTACTT11.5384615384615385No Hit
GAGGTGTTCTGGGACTGGTGCACGAGCAGCGTCTTCACGATGGAGCGGATG11.5384615384615385No Hit
TTTTTGATCTTGTGGATCCGTTGAACGAACGTAACTATGAAGGAGGACTGT11.5384615384615385No Hit
CTAACTCTCCTTTACCAAGCTTCTGCGTTTTCACATCTGAGTACAAGAACT11.5384615384615385No Hit
GTAGTAGGTCGCACCACCGGTCAGCATGGTGCTCTTGAAGCCCTTGCCCGC11.5384615384615385No Hit
ATGTTGAATTTGACAAAGCACTCGTCGTATATGATCTACAATGTCTGCCTC11.5384615384615385No Hit
CTTGGCGCCGTCGAACACCGGGGTCGACACGATCTGGTTCGGCGCAGCGTG11.5384615384615385No Hit
CACTAAAACCGTGTATCCTATCGAAAAGGTAACTGTGGAAAGCCAGTGTTA11.5384615384615385No Hit
ACCAACACCTAAGATGTTTGGTTTCTTTATCCACCTAAGATGTTTGGCTTC11.5384615384615385No Hit
GAGTTCCAATGATTCTTAATGGCATTATCTGTTCTCCCAGGTAAGAGTTTA11.5384615384615385No Hit
CTACTAGATGGTTCGATTAGTCTTTCGCCCCTATACCCCTGTCTCTTATAC11.5384615384615385No Hit
GTATTATTCGATGGAAATCGATTCACAGGAAGCATACCTTCCACTTTAGGA11.5384615384615385No Hit
GAAAAAAAGGAGGAAAAAAACGCTAACTCTCACGTCCTGCCGTAGAGGAAA11.5384615384615385No Hit
ATACAGTACACAAACGTCTGCAGCAGTTTTCATCTGAATGACATTAATGGC11.5384615384615385No Hit
TATCAGCACTGCTGATGCCTAAGTTGTTTTCATTGTGTTTATTAGTCCATT11.5384615384615385No Hit
CCATACAAGCCTCCTCACTACAGGTACTGAGAGAGAGAGCTGTCTCTTATA11.5384615384615385No Hit
ACACTACTTAATCCCCCTTAATTGGTGGCTTTAATTGCTGTCTCTTATACA11.5384615384615385No Hit
GTATTTCAGGAGTCTGGAGCCAATTTGTTCATAACTGCCCCAATAGGTTCA11.5384615384615385No Hit
GTTTCTTAGGTTCGAAGTTTTCAAGGATAATCTAAAGAACATCGATGAGAC11.5384615384615385No Hit
CAACTTGAGCGCATCAACGTTTACTACAATGAAGCGAGTTGCGGTAGATTC11.5384615384615385No Hit
ACTTTCTTTACTACAACACTGATATCTTCATTTGTTGCCCCTCGAACATAT11.5384615384615385No Hit
AGCTATGATGCCACGATCGCGCGTCCCGTCTCGGAGCGATCGTACGGCGAT11.5384615384615385No Hit
CAAGAAGATTGCGGTTCTCGGTTTCGCATTCAAGAAAGACACCGGTGACAC11.5384615384615385No Hit
ATGTCGGTGTGTGAAAATTTGGTAAGATTGTGATCTCTGTGTTTACGACAA11.5384615384615385No Hit
ACTCATTTTTGTAAGGGAAACATGAGAATGCCCTGCCTTTGTCGAGGACGG11.5384615384615385No Hit
ATATTCTGTAAATGGTAAAGATGAGAAGTGATCCACCATTCTTGTCAACAG11.5384615384615385No Hit
ATCCAACAATGTGTGAAGCCATTTTGATTTTCTTTTACTGACGGAGGAAGA11.5384615384615385No Hit
GGTTACATCAGGCTGAAGAGGAACACTGGGAAACCAGAGGGTCTCTGTGGA11.5384615384615385No Hit
AACCGCCACGTCCCGGTTCCGGAATTTTAACCGGATCCCCTTTCGAAGTTC11.5384615384615385No Hit
ATGTATCAATTTGTGTTCTGTTTCTATCTCCACTCTGATCATAAACTGCCA11.5384615384615385No Hit
GTAAACGCAATGAGATACTTTGGCTTCACAGGAACATCCTCCTCAGGTAAT11.5384615384615385No Hit
TACCACTTCTGTTTCAGCGTTTTCTGAAGTAGAAGAAGAACATTGAAGAGA11.5384615384615385No Hit
GCTATAGCTGTAGCCGGTTGAACCACCCGGGCCAGTGATGGAGGCTGTCTC11.5384615384615385No Hit
GCCTCTCATAAATCAAAAACAACCCCTTATTCATACCAATATTTTGTCACA11.5384615384615385No Hit
AGGGAAACACCTGCCATTGATGTGTGTAAAGGTCTATTAGGAGACAAAGCA11.5384615384615385No Hit
TTAGTGCATTGACCAATATTCTTCACTGCTGCGCATCGGTTGATGCTTTGG11.5384615384615385No Hit
TGCATTCTCTGAATCAAAGGGCAAGTCGTGAGAGGTATGTTCATAACCTGT11.5384615384615385No Hit
CATTCGACCCGTCTTGAAACACGGACCAAGGAGTCTGACATGTGTGCGAGT11.5384615384615385No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTTA11.5384615384615385No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTTTC50.045.019
GTTTGGT50.045.016
GTTTGGC50.045.042
CCAACAC50.045.02
CTTTATC50.045.025
ATCCACC50.045.029
TTTCTTT50.045.022
TTATCCA50.045.027
ACACCTA50.045.05
GTTTCTT50.045.021
GGTTTCT50.045.020
ACCAACA50.045.01
CAACACC50.045.03
CCACCTA50.045.031
TTCTTTA50.045.023
TATCCAC50.045.028
TCCACCT50.045.030
TTTGGTT50.045.017
TTGGCTT50.045.044
TTGGTTT50.045.018
Produced by FastQC (version 0.11.2)