FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_4_91.3410000000b2c5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_4_91.3410000000b2c5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53
Sequences flagged as poor quality0
Sequence length51
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTC11.8867924528301887No Hit
GAGTTAGGGTTATTGGCTGGTGTTAAGGAAGAGAATCATCAAGAGAGGAAC11.8867924528301887No Hit
CCCAGGCTCTGTTATTTCCTCTTTGCTTCGTTGAACGAGAAAATCCATGAA11.8867924528301887No Hit
ACACTGGAAATGTCCGTCAAACTCTACAAAGGAAATATCATCTTCAGAATG11.8867924528301887No Hit
GTACCCGAGGCGGTACCGCCACCCTTCTGAGGCCAAGGAACCTCCCATGAG11.8867924528301887No Hit
TCTTAGAGCTGAGATTAAATCAGGTTCTGAAAATGGAACTATGATCCAGAA11.8867924528301887No Hit
TCTCATCATTAGTAGGTACGTCTTGGTGTCCATTAATGGTTACTGTTTCAG11.8867924528301887No Hit
CCATCAGCTAAGGTCCCTAAATGATCGCTCAGTGGTTAAGGATGTGTCTGT11.8867924528301887No Hit
GTATCAACGCAGAGTACGGGACAATTAGCCTAAGTTACATGCACCTTCGAT11.8867924528301887No Hit
CTTCTGGCCGCTGCGCGCGCCGCCGGGGCTGGGCGTGCCCTGGCTGTTCAG11.8867924528301887No Hit
GACAATGGTAGACTCATGATGCATCAAAACATTTCGAGGCTTAATTACCTC11.8867924528301887No Hit
CAATCAGAACTCTCTTGATCGCGGGCATCGCGATCACTTCTGGTATCCCGG11.8867924528301887No Hit
ATTCGAATGAGTCAAATTCAACAGCGATACATAATAGAGAGATAAAAGACC11.8867924528301887No Hit
CTGTAGAGCAGAAACTATACGTCTTATAGGATTCCTTTGATTGTGAGATAG11.8867924528301887No Hit
CTTCAAAGATTAGGAAGCCGGTTTGAGAATGTCGCAGTCCATCATGATAGA11.8867924528301887No Hit
AGAGAGAGAAGTAGAGCGATTATGGATTTGAAGTGAGAAGGAAGGAAAATG11.8867924528301887No Hit
GCGTCACTGTGAATAGCAACAAGCGAAAGCCATTCCTTTTCAGAGAGCCCA11.8867924528301887No Hit
CAATAGAATCAATCAATCTGAACAAAGAAACAGAAGGATCATGTCTGTCTC11.8867924528301887No Hit
CGTATAGGGCAGCGCGCGGCCCGGCGTGATCGCGCGCGGCTGTGCCAGCGA11.8867924528301887No Hit
ATGGGACACAATGAAAGTAGTCCTAAGAGGAAAACTCATATCCCCGAGTGC11.8867924528301887No Hit
CTGTGGCTGGATGCGCATGCCGACTTCAACACCAGCGAGCTCACGCCCAGC11.8867924528301887No Hit
GAATGGTGGTGGCGCAGCCGAGGATCTTCGTCGGGGCGGTGCGGCGGTCTT11.8867924528301887No Hit
GAGCTCAGGCGAATCCTTACAGCCGTGGATGCAGCACCATCACTAGGTGCA11.8867924528301887No Hit
GCGTTAGACGATGGAATGAGATGCTTATCAATGACAGACAAGATGTCATTA11.8867924528301887No Hit
TCCCTTCTTCTTGTTGCTCAACCAGCACAAGATTATCGCCTTTCTCAACAA11.8867924528301887No Hit
CTCACAGACTCACTCATGAAGATTCTCACTGAGAGAGGTTACATGCTGTCT11.8867924528301887No Hit
CTTTAGAACAGTCCAACAACAATGGCGTTTTAACCTCGTGACCTCGACTAT11.8867924528301887No Hit
GGTTAAGGTAATAAGCCATGACATAATCGACAATAAGGACAGCCGCATGAC11.8867924528301887No Hit
ACCTCGCATCGGCGATGCAGCGCGATCCGGCCTTGCTGCGCGACGCGGCGG11.8867924528301887No Hit
CAGCAGGCGCGGCGCAGCATGAGGTTTCAGGCCTTCCTGGGGACATTCGCG11.8867924528301887No Hit
ACATAAACCAACCACAACGAGTAGAGATAGTGTACTTCTCGATCTGTTATT11.8867924528301887No Hit
GCCAGAACCAGTGAGAACCCACTTGAGGAACATGATCATTGTGCCTGAAAT11.8867924528301887No Hit
CCCGACTACCTGTCGCAGGACTTCACCCGCATCACCCCCACGCACTACCTG11.8867924528301887No Hit
ACAAAGATGGGGTTTTGATGGCTTCTGATATGGGAGGTTCATATGGATCAA11.8867924528301887No Hit
CCAGATAGGAATAGGACGATCATCAGAGAATTGACGATAGAGAACCAGTGG11.8867924528301887No Hit
GTATTGGCCAGCTCCGCCAGGAAACATCGTGCCACCGCCTGGGAAAGTAAA11.8867924528301887No Hit
CTTATACACATTCTCAAACAGATAGTCCTGAAACCTAGTAACAAACTTCCT11.8867924528301887No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC11.8867924528301887RNA PCR Primer, Index 23 (95% over 21bp)
CCCTTCTCCCCTGTGCATAAAGCCCTGAAATTTTCTACAGTTTTGGGTACA11.8867924528301887No Hit
CTTCCCAGAAGTCGGCAGAGCTCTGCGCACCGGCGAGAGGCATCTTCTTAA11.8867924528301887No Hit
AACTGGTGCTACCGACATTCCCTTCTTGTTAACAAATGGTTCAAGAACAGA11.8867924528301887No Hit
GATTCATAGCTCAGAGCAAGAAGGAACAGGGAGAGAGTGCTCAGGGCCAAT11.8867924528301887No Hit
AACCCGGATGGGTTCCGGTAAAGGCAACCCCGAATTTTGGGTAGCCGTCGT11.8867924528301887No Hit
CAAATATTGTGTTCACAAGTTTTCGACTTTAGAGTTCCTGCCTGAACCAAC11.8867924528301887No Hit
GACGTGGCCCTTGCGGCGGTGTGGTCAAATAGCGACTTTGCGGGTAGACAC11.8867924528301887No Hit
ATCAATCAACTGGTAATCAACACGGTTCACATGTGGAACATCACAATCTGT11.8867924528301887No Hit
AATGTGGGATCTCCTCCTCGATATGATCCAGGAAATATACTTCAACCATAT11.8867924528301887No Hit
GGCCGCACGTGACGGCCTACGCGCTGGGCTACTGCGCGTCGACCAACAACA11.8867924528301887No Hit
ATGCACTGATCACCTGAACCTGATCCCATGGCCAGCAACACCGAGCTCTTC11.8867924528301887No Hit
CCACCAGATTGCCCCGTGGGCAAGCCTATGGTACCTCTCCTTGATTGATGA11.8867924528301887No Hit
ACACTGTTGTTGGATTTTTGATGGCTGGAGTTGGTAATGTTGACATAAGGA11.8867924528301887No Hit
ATTTACTTCTTTGGTTAGCTCTAAAGATCGACTCATATCTCTCATAGCTGT11.8867924528301887No Hit
TTCAACCCAAAACCAAAAACAGAAAAATAAATCTGAACTTTTCTTTCACAT11.8867924528301887No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATGCA50.045.017
GTAGACT50.045.08
CATGATG50.045.015
CAATGGT50.045.03
TGCATCA50.045.020
TTACCTC50.045.045
AAACATT50.045.027
TGGTAGA50.045.06
ATGATGC50.045.016
GATGCAT50.045.018
TTCGAGG50.045.033
ATGGTAG50.045.05
AATTACC50.045.043
CATTTCG50.045.030
ACATTTC50.045.029
CATCAAA50.045.022
CGAGGCT50.045.035
ACTCATG50.045.012
ATTACCT50.045.044
TCAAAAC50.045.024
Produced by FastQC (version 0.11.2)