##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23V3BCXX l02n01 kb2_4_90.3410000000b2b8.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 29 Sequences flagged as poor quality 0 Sequence length 51 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.241379310344826 0.0 0.0 0.0 0.0 0.0 2 36.55172413793103 0.0 0.0 0.0 0.0 0.0 3 35.3448275862069 0.0 0.0 0.0 0.0 0.0 4 36.37931034482759 0.0 0.0 0.0 0.0 0.0 5 36.13793103448276 0.0 0.0 0.0 0.0 0.0 6 37.310344827586206 0.0 0.0 0.0 0.0 0.0 7 37.96551724137931 0.0 0.0 0.0 0.0 0.0 8 37.58620689655172 0.0 0.0 0.0 0.0 0.0 9 35.89655172413793 0.0 0.0 0.0 0.0 0.0 10 36.3448275862069 0.0 0.0 0.0 0.0 0.0 11 36.96551724137931 0.0 0.0 0.0 0.0 0.0 12 36.13793103448276 0.0 0.0 0.0 0.0 0.0 13 37.03448275862069 0.0 0.0 0.0 0.0 0.0 14 37.44827586206897 0.0 0.0 0.0 0.0 0.0 15 35.827586206896555 0.0 0.0 0.0 0.0 0.0 16 36.55172413793103 0.0 0.0 0.0 0.0 0.0 17 38.0 0.0 0.0 0.0 0.0 0.0 18 38.206896551724135 0.0 0.0 0.0 0.0 0.0 19 38.48275862068966 0.0 0.0 0.0 0.0 0.0 20 37.51724137931034 0.0 0.0 0.0 0.0 0.0 21 36.58620689655172 0.0 0.0 0.0 0.0 0.0 22 37.51724137931034 0.0 0.0 0.0 0.0 0.0 23 37.62068965517241 0.0 0.0 0.0 0.0 0.0 24 37.172413793103445 0.0 0.0 0.0 0.0 0.0 25 37.37931034482759 0.0 0.0 0.0 0.0 0.0 26 36.206896551724135 0.0 0.0 0.0 0.0 0.0 27 38.310344827586206 0.0 0.0 0.0 0.0 0.0 28 37.96551724137931 0.0 0.0 0.0 0.0 0.0 29 37.62068965517241 0.0 0.0 0.0 0.0 0.0 30 37.96551724137931 0.0 0.0 0.0 0.0 0.0 31 37.827586206896555 0.0 0.0 0.0 0.0 0.0 32 36.03448275862069 0.0 0.0 0.0 0.0 0.0 33 36.724137931034484 0.0 0.0 0.0 0.0 0.0 34 36.41379310344828 0.0 0.0 0.0 0.0 0.0 35 38.13793103448276 0.0 0.0 0.0 0.0 0.0 36 36.86206896551724 0.0 0.0 0.0 0.0 0.0 37 36.793103448275865 0.0 0.0 0.0 0.0 0.0 38 37.48275862068966 0.0 0.0 0.0 0.0 0.0 39 37.689655172413794 0.0 0.0 0.0 0.0 0.0 40 37.96551724137931 0.0 0.0 0.0 0.0 0.0 41 38.275862068965516 0.0 0.0 0.0 0.0 0.0 42 37.41379310344828 0.0 0.0 0.0 0.0 0.0 43 38.41379310344828 0.0 0.0 0.0 0.0 0.0 44 36.206896551724135 0.0 0.0 0.0 0.0 0.0 45 37.06896551724138 0.0 0.0 0.0 0.0 0.0 46 35.51724137931034 0.0 0.0 0.0 0.0 0.0 47 35.724137931034484 0.0 0.0 0.0 0.0 0.0 48 38.827586206896555 0.0 0.0 0.0 0.0 0.0 49 37.55172413793103 0.0 0.0 0.0 0.0 0.0 50 36.62068965517241 0.0 0.0 0.0 0.0 0.0 51 37.55172413793103 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2112 1 -3.0 2112 2 0.0 2112 3 -3.0 2112 4 NaN 2112 5 0.0 2112 6 -4.0 2112 7 -4.0 2112 8 -1.0 2112 9 -1.0 2112 10 -3.0 2112 11 -1.0 2112 12 -1.0 2112 13 -1.0 2112 14 -1.0 2112 15 -1.0 2112 16 1.0 2112 17 -4.0 2112 18 -1.0 2112 19 -4.0 2112 20 -1.0 2112 21 -4.0 2112 22 NaN 2112 23 -3.0 2112 24 -4.0 2112 25 -1.0 2112 26 -1.0 2112 27 -1.0 2112 28 -1.0 2112 29 NaN 2112 30 -4.0 2112 31 -1.0 2112 32 -1.0 2112 33 -1.0 2112 34 -1.0 2112 35 -1.0 2112 36 -1.0 2112 37 -1.0 2112 38 -1.0 2112 39 -1.0 2112 40 0.0 2112 41 -1.0 2112 42 0.0 2112 43 -1.0 2112 44 -1.0 2112 45 0.0 2112 46 -4.0 2112 47 -4.0 2112 48 -1.0 2112 49 NaN 2112 50 -4.0 2112 51 -1.0 1115 1 3.0 1115 2 0.0 1115 3 3.0 1115 4 NaN 1115 5 0.0 1115 6 4.0 1115 7 4.0 1115 8 1.0 1115 9 1.0 1115 10 3.0 1115 11 1.0 1115 12 1.0 1115 13 1.0 1115 14 1.0 1115 15 1.0 1115 16 -1.0 1115 17 4.0 1115 18 1.0 1115 19 4.0 1115 20 1.0 1115 21 4.0 1115 22 NaN 1115 23 3.0 1115 24 4.0 1115 25 1.0 1115 26 1.0 1115 27 1.0 1115 28 1.0 1115 29 NaN 1115 30 4.0 1115 31 1.0 1115 32 1.0 1115 33 1.0 1115 34 1.0 1115 35 1.0 1115 36 1.0 1115 37 1.0 1115 38 1.0 1115 39 1.0 1115 40 0.0 1115 41 1.0 1115 42 0.0 1115 43 1.0 1115 44 1.0 1115 45 0.0 1115 46 4.0 1115 47 4.0 1115 48 1.0 1115 49 NaN 1115 50 4.0 1115 51 1.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 28 1.0 29 0.0 30 1.0 31 0.0 32 1.0 33 1.0 34 0.0 35 2.0 36 2.0 37 6.0 38 8.0 39 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.827586206896555 24.137931034482758 3.4482758620689653 27.586206896551722 2 10.344827586206897 31.03448275862069 41.37931034482759 17.24137931034483 3 6.896551724137931 20.689655172413794 34.48275862068966 37.93103448275862 4 20.689655172413794 13.793103448275861 34.48275862068966 31.03448275862069 5 13.793103448275861 20.689655172413794 37.93103448275862 27.586206896551722 6 34.48275862068966 34.48275862068966 17.24137931034483 13.793103448275861 7 17.24137931034483 31.03448275862069 34.48275862068966 17.24137931034483 8 10.344827586206897 51.724137931034484 24.137931034482758 13.793103448275861 9 24.137931034482758 13.793103448275861 10.344827586206897 51.724137931034484 10 17.24137931034483 20.689655172413794 34.48275862068966 27.586206896551722 11 24.137931034482758 13.793103448275861 27.586206896551722 34.48275862068966 12 27.586206896551722 6.896551724137931 20.689655172413794 44.827586206896555 13 31.03448275862069 24.137931034482758 34.48275862068966 10.344827586206897 14 13.793103448275861 24.137931034482758 20.689655172413794 41.37931034482759 15 31.03448275862069 34.48275862068966 3.4482758620689653 31.03448275862069 16 13.793103448275861 34.48275862068966 27.586206896551722 24.137931034482758 17 20.689655172413794 17.24137931034483 41.37931034482759 20.689655172413794 18 37.93103448275862 34.48275862068966 17.24137931034483 10.344827586206897 19 17.24137931034483 20.689655172413794 31.03448275862069 31.03448275862069 20 20.689655172413794 24.137931034482758 27.586206896551722 27.586206896551722 21 24.137931034482758 34.48275862068966 17.24137931034483 24.137931034482758 22 34.48275862068966 27.586206896551722 31.03448275862069 6.896551724137931 23 24.137931034482758 24.137931034482758 31.03448275862069 20.689655172413794 24 31.03448275862069 20.689655172413794 17.24137931034483 31.03448275862069 25 34.48275862068966 20.689655172413794 20.689655172413794 24.137931034482758 26 20.689655172413794 27.586206896551722 27.586206896551722 24.137931034482758 27 34.48275862068966 13.793103448275861 27.586206896551722 24.137931034482758 28 27.586206896551722 17.24137931034483 34.48275862068966 20.689655172413794 29 34.48275862068966 24.137931034482758 24.137931034482758 17.24137931034483 30 20.689655172413794 24.137931034482758 17.24137931034483 37.93103448275862 31 37.93103448275862 10.344827586206897 31.03448275862069 20.689655172413794 32 34.48275862068966 31.03448275862069 20.689655172413794 13.793103448275861 33 20.689655172413794 27.586206896551722 44.827586206896555 6.896551724137931 34 10.344827586206897 20.689655172413794 37.93103448275862 31.03448275862069 35 24.137931034482758 24.137931034482758 34.48275862068966 17.24137931034483 36 17.24137931034483 3.4482758620689653 34.48275862068966 44.827586206896555 37 17.24137931034483 24.137931034482758 31.03448275862069 27.586206896551722 38 34.48275862068966 13.793103448275861 31.03448275862069 20.689655172413794 39 27.586206896551722 17.24137931034483 27.586206896551722 27.586206896551722 40 24.137931034482758 31.03448275862069 24.137931034482758 20.689655172413794 41 27.586206896551722 20.689655172413794 31.03448275862069 20.689655172413794 42 27.586206896551722 17.24137931034483 34.48275862068966 20.689655172413794 43 27.586206896551722 17.24137931034483 20.689655172413794 34.48275862068966 44 10.344827586206897 41.37931034482759 24.137931034482758 24.137931034482758 45 13.793103448275861 20.689655172413794 41.37931034482759 24.137931034482758 46 27.586206896551722 20.689655172413794 34.48275862068966 17.24137931034483 47 37.93103448275862 13.793103448275861 31.03448275862069 17.24137931034483 48 10.344827586206897 34.48275862068966 27.586206896551722 27.586206896551722 49 37.93103448275862 20.689655172413794 20.689655172413794 20.689655172413794 50 27.586206896551722 24.137931034482758 27.586206896551722 20.689655172413794 51 20.689655172413794 31.03448275862069 27.586206896551722 20.689655172413794 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.5 29 1.0 30 2.0 31 3.0 32 3.0 33 3.0 34 2.5 35 2.0 36 1.5 37 1.0 38 2.0 39 3.0 40 1.5 41 0.0 42 0.5 43 1.0 44 1.0 45 1.0 46 1.0 47 1.0 48 0.5 49 0.0 50 0.0 51 0.0 52 0.5 53 1.0 54 0.5 55 0.0 56 0.5 57 1.0 58 1.0 59 1.0 60 1.5 61 2.0 62 1.0 63 0.0 64 1.5 65 3.0 66 2.0 67 1.0 68 1.5 69 2.0 70 1.5 71 1.0 72 0.5 73 0.0 74 0.5 75 0.5 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 29.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 100.0 #Duplication Level Percentage of deduplicated Percentage of total 1 100.0 100.0 2 0.0 0.0 3 0.0 0.0 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATTAGAATTCAGCAGAACAGTCCATGCTGATCTTAGTAACTATGATGAA 1 3.4482758620689653 No Hit ATTGCACTCCCGTCATAGGGTTCTTTTCACCTTTCCCTCACGGTACTTGTA 1 3.4482758620689653 No Hit CTCCTATTCAACTTCTAACAAAAAGCTAACTATTTCATCGTTAAAGTTCGG 1 3.4482758620689653 No Hit CACGTAAACCTCGACGTGTCGGGCCTGTGCGTTCTTTGGAACGAATGAGGC 1 3.4482758620689653 No Hit CCAGTGTTCTTATCAGTAATGACAACAGCTTCAACGATTTCACCAAACTGT 1 3.4482758620689653 No Hit CACCTGCAGACCACCCTGGCCTTCGCGCTGGAACAGTAGCGTCAGGCAGTC 1 3.4482758620689653 No Hit CTTATCTCCTCTCCCACACAAATCCATTTCCGATACCGCCGGAGCTGCACA 1 3.4482758620689653 No Hit GTATCAAATGTCACCATTTTAATGTTCACCATTTTTGTGACTGTTTGTGCA 1 3.4482758620689653 No Hit AGCTTCTTCATGTTGTCATCATACACATACGGAAGCTTATCTGTCTCTTAT 1 3.4482758620689653 No Hit GTCGCGCGACGCGGACGGCGCGGCCTTTTCGATCGCGCTGGTCAGTTCGTA 1 3.4482758620689653 No Hit ACTTCGAAGCCGGCAAACCGGTGGTCGAGGTGGCGCTCGGTTCCTGGGCCG 1 3.4482758620689653 No Hit GTGTGTACCCCCGAGCCGAGAAGCCAGCGGCGAAGAAGTGATCACCAAAGT 1 3.4482758620689653 No Hit GATGGTGACATCACGATTGCCTGCGGGGTAGATCACCCGCGTGCCGGTGAT 1 3.4482758620689653 No Hit GGCATATTCCGGTAATCAAAAACAAGGGCATGATTGGAACTGTCTCTTATA 1 3.4482758620689653 No Hit CCTCGCAGATCGCTGCGGCATCGGCCGCACCGTTCTTGCGTCCCTTGCCGG 1 3.4482758620689653 No Hit GTTTTATGCTCATAGCTGTCAGTGAGCTGATGTTATTTATTACTTAAAGTC 1 3.4482758620689653 No Hit GATCAGGAAAGCAAAAAAAAAATGTTATCTGAATCTGTCTCTTATACACAT 1 3.4482758620689653 No Hit ACCTTGTCCGCTTCCAGGTGGGCAGTGCTTACGCCGCGCCGAACCTGAAGG 1 3.4482758620689653 No Hit CAATATTTCTTTGCATCCTTTTGTTTCTGGTTGGTTCTGATGTGTGTGTAT 1 3.4482758620689653 No Hit GTGCACACTGCTGGGGTTGCCGAAGTCTTCCGCCATGGCTGCCATCGCCGC 1 3.4482758620689653 No Hit GACGAGGACTGCCTTTTCCGCTTCCAGGATGCCCTCCGGCAGGCCATGTCC 1 3.4482758620689653 No Hit AAACTTTTCTTTTCCATACCCTAGGGCGTATGTTTTTATTTTTTTGCAATG 1 3.4482758620689653 No Hit CATCATAAGCATGAGCTTGTGGATAATCAGTGTAGCTCTACGCTTGTTAAA 1 3.4482758620689653 No Hit GATACGGAGGACGACAGGCTAGCGTATTGGGTGGTCAGGGTCGAAAGCGCG 1 3.4482758620689653 No Hit GTCTCAAACTACATGAAGCTGTTGGGAGAAGATGAGCCAGAGAAGTTACAA 1 3.4482758620689653 No Hit TGTCAGCAGTGGTGATGATACCATGAGAGCCAGTGTCAAAGAAGAAGATGA 1 3.4482758620689653 No Hit GCCAGGCAGGCGGCACCGATGGCGGCCAGCGCGAGCAGCATGGAGCGCTTC 1 3.4482758620689653 No Hit ATCTCATATCGCTTGGTATATAAAGGCTGATATAACTTTTGATACTTAGCT 1 3.4482758620689653 No Hit ATACCAAAGAGGACATTTTTTGTTAATGGAATATTCACTAACCTTTCCGTT 1 3.4482758620689653 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.0 22 0.0 0.0 0.0 23 0.0 0.0 0.0 24 0.0 0.0 0.0 25 0.0 0.0 0.0 26 0.0 0.0 0.0 27 0.0 0.0 0.0 28 0.0 0.0 0.0 29 0.0 0.0 0.0 30 0.0 0.0 0.0 31 0.0 0.0 0.0 32 0.0 0.0 0.0 33 0.0 0.0 0.0 34 0.0 0.0 0.0 35 0.0 0.0 3.4482758620689653 36 0.0 0.0 3.4482758620689653 37 0.0 0.0 3.4482758620689653 38 0.0 0.0 3.4482758620689653 39 0.0 0.0 3.4482758620689653 >>END_MODULE >>Kmer Content pass >>END_MODULE