FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_4_90.3410000000b2b8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_4_90.3410000000b2b8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29
Sequences flagged as poor quality0
Sequence length51
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAGAATTCAGCAGAACAGTCCATGCTGATCTTAGTAACTATGATGAA13.4482758620689653No Hit
ATTGCACTCCCGTCATAGGGTTCTTTTCACCTTTCCCTCACGGTACTTGTA13.4482758620689653No Hit
CTCCTATTCAACTTCTAACAAAAAGCTAACTATTTCATCGTTAAAGTTCGG13.4482758620689653No Hit
CACGTAAACCTCGACGTGTCGGGCCTGTGCGTTCTTTGGAACGAATGAGGC13.4482758620689653No Hit
CCAGTGTTCTTATCAGTAATGACAACAGCTTCAACGATTTCACCAAACTGT13.4482758620689653No Hit
CACCTGCAGACCACCCTGGCCTTCGCGCTGGAACAGTAGCGTCAGGCAGTC13.4482758620689653No Hit
CTTATCTCCTCTCCCACACAAATCCATTTCCGATACCGCCGGAGCTGCACA13.4482758620689653No Hit
GTATCAAATGTCACCATTTTAATGTTCACCATTTTTGTGACTGTTTGTGCA13.4482758620689653No Hit
AGCTTCTTCATGTTGTCATCATACACATACGGAAGCTTATCTGTCTCTTAT13.4482758620689653No Hit
GTCGCGCGACGCGGACGGCGCGGCCTTTTCGATCGCGCTGGTCAGTTCGTA13.4482758620689653No Hit
ACTTCGAAGCCGGCAAACCGGTGGTCGAGGTGGCGCTCGGTTCCTGGGCCG13.4482758620689653No Hit
GTGTGTACCCCCGAGCCGAGAAGCCAGCGGCGAAGAAGTGATCACCAAAGT13.4482758620689653No Hit
GATGGTGACATCACGATTGCCTGCGGGGTAGATCACCCGCGTGCCGGTGAT13.4482758620689653No Hit
GGCATATTCCGGTAATCAAAAACAAGGGCATGATTGGAACTGTCTCTTATA13.4482758620689653No Hit
CCTCGCAGATCGCTGCGGCATCGGCCGCACCGTTCTTGCGTCCCTTGCCGG13.4482758620689653No Hit
GTTTTATGCTCATAGCTGTCAGTGAGCTGATGTTATTTATTACTTAAAGTC13.4482758620689653No Hit
GATCAGGAAAGCAAAAAAAAAATGTTATCTGAATCTGTCTCTTATACACAT13.4482758620689653No Hit
ACCTTGTCCGCTTCCAGGTGGGCAGTGCTTACGCCGCGCCGAACCTGAAGG13.4482758620689653No Hit
CAATATTTCTTTGCATCCTTTTGTTTCTGGTTGGTTCTGATGTGTGTGTAT13.4482758620689653No Hit
GTGCACACTGCTGGGGTTGCCGAAGTCTTCCGCCATGGCTGCCATCGCCGC13.4482758620689653No Hit
GACGAGGACTGCCTTTTCCGCTTCCAGGATGCCCTCCGGCAGGCCATGTCC13.4482758620689653No Hit
AAACTTTTCTTTTCCATACCCTAGGGCGTATGTTTTTATTTTTTTGCAATG13.4482758620689653No Hit
CATCATAAGCATGAGCTTGTGGATAATCAGTGTAGCTCTACGCTTGTTAAA13.4482758620689653No Hit
GATACGGAGGACGACAGGCTAGCGTATTGGGTGGTCAGGGTCGAAAGCGCG13.4482758620689653No Hit
GTCTCAAACTACATGAAGCTGTTGGGAGAAGATGAGCCAGAGAAGTTACAA13.4482758620689653No Hit
TGTCAGCAGTGGTGATGATACCATGAGAGCCAGTGTCAAAGAAGAAGATGA13.4482758620689653No Hit
GCCAGGCAGGCGGCACCGATGGCGGCCAGCGCGAGCAGCATGGAGCGCTTC13.4482758620689653No Hit
ATCTCATATCGCTTGGTATATAAAGGCTGATATAACTTTTGATACTTAGCT13.4482758620689653No Hit
ATACCAAAGAGGACATTTTTTGTTAATGGAATATTCACTAACCTTTCCGTT13.4482758620689653No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers