FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_4_9.3410000000ad2e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_4_9.3410000000ad2e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences720459
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTC11890.16503367991794118No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGCC1100.036.81676514
GCCCACG1050.036.42716218
TAGGTGG250.002106986735.998615
CCCACGA1200.031.8737719
TATGCCG1350.028.33224143
AGCCCAC1400.027.32037517
CGAGCCC1400.027.32037515
CGTATGC1450.026.37829241
CGCTATG1450.026.37829230
GATCTCG1550.026.12802536
GTATGCC1500.025.49901642
CTCGCTA1500.025.49901628
CTCGTAT1600.025.31152339
TCGCTAT1600.025.31152329
GAGCCCA1550.024.67646816
ATGCCGT1550.024.67646844
GACTCGC1550.024.67646826
TGCCGTC1600.023.90532745
CCACGAG1650.023.18092520
GTATCAA9650.023.1221281
Produced by FastQC (version 0.11.2)