FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_4_89.3410000000b2ac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_4_89.3410000000b2ac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56
Sequences flagged as poor quality0
Sequence length51
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAAGGCGTGACCGAAGTGGTCGTCGACAAGGATGTCGTCGATGGCCGC11.7857142857142856No Hit
GTAAAAAAAGTTGAAGAAGCATACAACTTCATCAAACAAGTATCTGAAGAC11.7857142857142856No Hit
ATCAGTAGCTGAATCAACCAAGCTGAAGAAGGGATATCAGAGTTTGAAGAC11.7857142857142856No Hit
ATGCGGAGGCGCGACGAATAGGCTGCTTGGTATTAACGACCATCGGTAGCA11.7857142857142856No Hit
GCCTTCTGACAAGCTATGAATTCACTTGTCGATAACACAACATAACTTGTG11.7857142857142856No Hit
TACTAGGAATTCCTCGTTGAAGACCAACAATTGCAATGATCTATCCCCATC11.7857142857142856No Hit
TGATTGTTCAAGACTAAAATGCAAAAAACACAAAAGGTTCAAAACTGATTC11.7857142857142856No Hit
TATCAGAAGTGAGAATGCTGACATGAGTAACGATAAATCTGTCTCTTATAC11.7857142857142856No Hit
CTCTATTGCACTCCCGTCATAGGGTTCTTTTCACCTTTCCCTCACGGTACT11.7857142857142856No Hit
CATACCAACAATTTGGAAATCAATCATACGCTCATCAACATCTACGTTAAC11.7857142857142856No Hit
CTATTTGTGCTCACTTGTGTTACCCTTAATACTTTTTGTTAGCTTCTCTCT11.7857142857142856No Hit
GTTCGTGCGGGTGCGCGGGCATGCCCGTGAACTGCCGGTGAGCCGGGCTTA11.7857142857142856No Hit
ATTCACACAAACACGAGATAATAGAAATCCCACATAAAACCGACAACCTTC11.7857142857142856No Hit
GGGTTGAAGATGTAATGAATAAAATGGGCGATGAGATAGTTGCAAATCTGT11.7857142857142856No Hit
AGGCATTACATACCATAAGAAAGCAAAACAAGAACAATGTAATAATACCTG11.7857142857142856No Hit
ACTCAAATTCAAAAGAACGTCACAACCGTTTTAACATTAGGAGACTCTGTC11.7857142857142856No Hit
ATACACAAGTATCAGTAGCTGAATCAACCAAGCTGAAGAAGGGATATCAGA11.7857142857142856No Hit
ATACATTGTCTCACTCTCAGAGGTGGCCCTTGAGTTTATTTGCCTGTCTCT11.7857142857142856No Hit
GACATACCACTTGTTTTTATTTTTCTCTGTGTTTGGTTGCCGTAGAAGAGC11.7857142857142856No Hit
CATCAGTCTCATCTCTCAAATCTTGTCTCTTCTTAGTCTGTTTCTGTTTGT11.7857142857142856No Hit
CTCTTTGGACTTTGAAAACTTAGCGAACTGAAACATCTAAGTAGCTAAAGC11.7857142857142856No Hit
TCTTTTAGGATTTCAGAATGAGGTTCAAAAGGAAACACAGACAACAAAACT11.7857142857142856No Hit
CCTAAAAACCTTAAGATGAAACTTGGATCGTGAAAACGAAATTTGCTACTT11.7857142857142856No Hit
GATTATAAACAAGCTGGTGCAGAAATAGTTAACGATGCAAAAAACAACTTG11.7857142857142856No Hit
CATCGAATCGTTTATGTTAGCAGCTAATGAAACGATAGCAGAACACTTTGA11.7857142857142856No Hit
GTCGTAAGAATTCCAACAAGAGCCTTGATCGTAAGCTGCATGGTTCAGAGT11.7857142857142856No Hit
CTCTAAATCCCCTTGTTAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTT11.7857142857142856No Hit
ATGATATACAACTTCTAATTCATATTGTGCAGAAACACCCTGTCTCTTACA11.7857142857142856No Hit
GCATAAGAACTCACTAGAGGCTTTTCTCGGCAGCATAGGATCACTAACTTC11.7857142857142856No Hit
ATGATAAATTGAATGATGTTTACGATAGCTTACATGTCTGTCTCTTATACA11.7857142857142856No Hit
TCTTATACGCATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGT11.7857142857142856No Hit
CAACAAGTGATTGTTCGTTATTTTCCTCTCCCACTTTAATGTCCAAGAAAT11.7857142857142856No Hit
CCCTCGAACTGGCTGCAACGTTCGAGCCTGGCATCCGCCATGATTTCAACG11.7857142857142856No Hit
TTTGTAAAAGCTTTGGCTACTTTTAGTGAATTAAAAACGTCTGTCTCTTAT11.7857142857142856No Hit
TATTAGAACTATGGGTGCACAAGGATTAAAAGAAGTGTCTGAAGCAGCTGT11.7857142857142856No Hit
ATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTCTTC11.7857142857142856RNA PCR Primer, Index 23 (95% over 24bp)
TGCAGAAACCCAGCAAAGTTTATCATTTCCATCTTGTTTCAAAATGTTCAT11.7857142857142856No Hit
TTACTAAGCATAAGATCAAAAAAGAAGCCCATAGAGAAGCATTGATGTGTA11.7857142857142856No Hit
GACATGTGGAGCGCTTAGAAGTGAGAATGCCGGTGTGAGTAGCGAAAGACG11.7857142857142856No Hit
ATCTTCGGACAATGTCCTTAAGAATGAAATCATCATCATGACAAATATAGC11.7857142857142856No Hit
GCAGTAAGCCATTATCATACCACTACCCTATAGCTGTCTCTTATACACATC11.7857142857142856No Hit
ATACACCAATGGCTAGACCTAACTTTGCATTCACTTTAGTCTTCCCGTACT11.7857142857142856No Hit
TAGCTGGAATAGCTGTCGGTCGACACCGCGGTCTGCAGCAGGTGCATCAGC11.7857142857142856No Hit
TTCCTGTACCACCTATAATCGTTTTAATCGATGGGGGGACGCAGTAGGATC11.7857142857142856No Hit
CTCCCATTCTTTCAGCATCTGCTTCCGCTTGTCTGATAGCAAGTGCCTCTT11.7857142857142856No Hit
GAATAAACCGGTTGTTAGTTGTTCAGGTTACTACTCCGATACCTGTCTCTT11.7857142857142856No Hit
CTAATTTCCCTATCTTCTCTATTCTTTTCTTCGCCTTCCCGTACCTGTCTC11.7857142857142856No Hit
GTTACTACCTTGTTCGTCATTATCTACAACCATCTTCTCACCTGACGCATT11.7857142857142856No Hit
GGGTATCTGGAAACGTAATGTTAAGTCACTTGTCATTCTCTAAATCTGTCT11.7857142857142856No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCTGTCTCTTATACA11.7857142857142856No Hit
TGGTTGTGGACGATGCGGCGATGGGAATCACACACGTCGTTCGGGGTGACG11.7857142857142856No Hit
GTGCTGTTGGGTTTCGAGCCCGAGCCATCGGCCTTCGCGCTGCTGGACGAG11.7857142857142856No Hit
GCCTATGCGCGCCAGTTCTCCGAAGCCTCGACGGCGGCGGTCGGCGGCGAT11.7857142857142856No Hit
TCTTAGAAAAGGTTCAACACTTGGTGGTGATTAGGAATTGTGACTGTCACC11.7857142857142856No Hit
AATTAATGTCTGGCAGTGATGTTAAAGCTTTGCACTCATCAATTAGGGAAT11.7857142857142856No Hit
AGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTG11.7857142857142856No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCATA50.045.015
CTTTCCC50.045.035
CTTTTCA50.045.027
TGCACTC50.045.07
TTCACCT50.045.030
TTGCACT50.045.06
GGGTTCT50.045.022
GTCATAG50.045.016
CTCCCGT50.045.011
TTTCCCT50.045.036
GTTCTTT50.045.024
ATAGGGT50.045.019
AGGGTTC50.045.021
TTTTCAC50.045.028
GGTTCTT50.045.023
TTTCACC50.045.029
ACTCCCG50.045.010
CCGTCAT50.045.014
TCTATTG50.045.02
CTCTATT50.045.01
Produced by FastQC (version 0.11.2)