##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23V3BCXX l02n01 kb2_4_88.3410000000b29f.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 58 Sequences flagged as poor quality 0 Sequence length 51 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.96551724137931 0.0 0.0 0.0 0.0 0.0 2 36.98275862068966 0.0 0.0 0.0 0.0 0.0 3 35.706896551724135 0.0 0.0 0.0 0.0 0.0 4 36.44827586206897 0.0 0.0 0.0 0.0 0.0 5 36.86206896551724 0.0 0.0 0.0 0.0 0.0 6 38.775862068965516 0.0 0.0 0.0 0.0 0.0 7 38.37931034482759 0.0 0.0 0.0 0.0 0.0 8 37.6551724137931 0.0 0.0 0.0 0.0 0.0 9 38.39655172413793 0.0 0.0 0.0 0.0 0.0 10 38.48275862068966 0.0 0.0 0.0 0.0 0.0 11 36.706896551724135 0.0 0.0 0.0 0.0 0.0 12 37.741379310344826 0.0 0.0 0.0 0.0 0.0 13 37.810344827586206 0.0 0.0 0.0 0.0 0.0 14 38.37931034482759 0.0 0.0 0.0 0.0 0.0 15 37.172413793103445 0.0 0.0 0.0 0.0 0.0 16 38.241379310344826 0.0 0.0 0.0 0.0 0.0 17 38.293103448275865 0.0 0.0 0.0 0.0 0.0 18 38.05172413793103 0.0 0.0 0.0 0.0 0.0 19 38.06896551724138 0.0 0.0 0.0 0.0 0.0 20 38.3448275862069 0.0 0.0 0.0 0.0 0.0 21 38.10344827586207 0.0 0.0 0.0 0.0 0.0 22 38.10344827586207 0.0 0.0 0.0 0.0 0.0 23 37.827586206896555 0.0 0.0 0.0 0.0 0.0 24 38.53448275862069 0.0 0.0 0.0 0.0 0.0 25 38.62068965517241 0.0 0.0 0.0 0.0 0.0 26 37.689655172413794 0.0 0.0 0.0 0.0 0.0 27 37.43103448275862 0.0 0.0 0.0 0.0 0.0 28 37.98275862068966 0.0 0.0 0.0 0.0 0.0 29 37.53448275862069 0.0 0.0 0.0 0.0 0.0 30 38.189655172413794 0.0 0.0 0.0 0.0 0.0 31 38.327586206896555 0.0 0.0 0.0 0.0 0.0 32 38.206896551724135 0.0 0.0 0.0 0.0 0.0 33 38.87931034482759 0.0 0.0 0.0 0.0 0.0 34 38.86206896551724 0.0 0.0 0.0 0.0 0.0 35 38.275862068965516 0.0 0.0 0.0 0.0 0.0 36 37.44827586206897 0.0 0.0 0.0 0.0 0.0 37 37.206896551724135 0.0 0.0 0.0 0.0 0.0 38 37.41379310344828 0.0 0.0 0.0 0.0 0.0 39 37.55172413793103 0.0 0.0 0.0 0.0 0.0 40 37.03448275862069 0.0 0.0 0.0 0.0 0.0 41 37.60344827586207 0.0 0.0 0.0 0.0 0.0 42 38.41379310344828 0.0 0.0 0.0 0.0 0.0 43 37.5 0.0 0.0 0.0 0.0 0.0 44 38.1551724137931 0.0 0.0 0.0 0.0 0.0 45 37.793103448275865 0.0 0.0 0.0 0.0 0.0 46 38.39655172413793 0.0 0.0 0.0 0.0 0.0 47 38.10344827586207 0.0 0.0 0.0 0.0 0.0 48 38.05172413793103 0.0 0.0 0.0 0.0 0.0 49 38.189655172413794 0.0 0.0 0.0 0.0 0.0 50 37.51724137931034 0.0 0.0 0.0 0.0 0.0 51 37.93103448275862 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2116 1 2.4000000000000004 2116 2 1.2000000000000002 2116 3 -2.4 2116 4 -3.5999999999999996 2116 5 -2.4 2116 6 NaN 2116 7 0.40000000000000036 2116 8 NaN 2116 9 NaN 2116 10 NaN 2116 11 NaN 2116 12 -0.7999999999999998 2116 13 NaN 2116 14 0.40000000000000036 2116 15 NaN 2116 16 -1.1999999999999993 2116 17 -1.1999999999999993 2116 18 0.40000000000000036 2116 19 -0.7999999999999998 2116 20 -0.7999999999999998 2116 21 NaN 2116 22 NaN 2116 23 NaN 2116 24 NaN 2116 25 -0.7999999999999998 2116 26 -0.7999999999999998 2116 27 -0.7999999999999998 2116 28 NaN 2116 29 NaN 2116 30 NaN 2116 31 NaN 2116 32 NaN 2116 33 -0.7999999999999998 2116 34 -0.7999999999999998 2116 35 NaN 2116 36 NaN 2116 37 NaN 2116 38 NaN 2116 39 NaN 2116 40 NaN 2116 41 0.40000000000000036 2116 42 -5.6 2116 43 -1.1999999999999993 2116 44 NaN 2116 45 -0.7999999999999998 2116 46 NaN 2116 47 NaN 2116 48 2.0 2116 49 -0.7999999999999998 2116 50 -4.4 2116 51 -4.4 1116 1 -3.5999999999999996 1116 2 -4.8 1116 3 -2.4 1116 4 -3.5999999999999996 1116 5 -2.4 1116 6 NaN 1116 7 -5.6 1116 8 NaN 1116 9 NaN 1116 10 NaN 1116 11 NaN 1116 12 -0.7999999999999998 1116 13 NaN 1116 14 -5.6 1116 15 NaN 1116 16 -1.1999999999999993 1116 17 -1.1999999999999993 1116 18 -5.6 1116 19 -0.7999999999999998 1116 20 -0.7999999999999998 1116 21 NaN 1116 22 NaN 1116 23 NaN 1116 24 NaN 1116 25 -0.7999999999999998 1116 26 -0.7999999999999998 1116 27 -0.7999999999999998 1116 28 NaN 1116 29 NaN 1116 30 NaN 1116 31 NaN 1116 32 NaN 1116 33 -0.7999999999999998 1116 34 -0.7999999999999998 1116 35 NaN 1116 36 NaN 1116 37 NaN 1116 38 NaN 1116 39 NaN 1116 40 NaN 1116 41 -5.6 1116 42 0.40000000000000036 1116 43 -1.1999999999999993 1116 44 NaN 1116 45 -0.7999999999999998 1116 46 NaN 1116 47 NaN 1116 48 -6.0 1116 49 -0.7999999999999998 1116 50 -4.4 1116 51 -4.4 1216 1 2.4000000000000004 1216 2 1.2000000000000002 1216 3 3.6 1216 4 2.4000000000000004 1216 5 3.6 1216 6 NaN 1216 7 2.4000000000000004 1216 8 NaN 1216 9 NaN 1216 10 NaN 1216 11 NaN 1216 12 1.2000000000000002 1216 13 NaN 1216 14 2.4000000000000004 1216 15 NaN 1216 16 0.8000000000000007 1216 17 0.8000000000000007 1216 18 2.4000000000000004 1216 19 1.2000000000000002 1216 20 1.2000000000000002 1216 21 NaN 1216 22 NaN 1216 23 NaN 1216 24 NaN 1216 25 1.2000000000000002 1216 26 1.2000000000000002 1216 27 1.2000000000000002 1216 28 NaN 1216 29 NaN 1216 30 NaN 1216 31 NaN 1216 32 NaN 1216 33 1.2000000000000002 1216 34 1.2000000000000002 1216 35 NaN 1216 36 NaN 1216 37 NaN 1216 38 NaN 1216 39 NaN 1216 40 NaN 1216 41 2.4000000000000004 1216 42 2.4000000000000004 1216 43 0.8000000000000007 1216 44 NaN 1216 45 1.2000000000000002 1216 46 NaN 1216 47 NaN 1216 48 2.0 1216 49 1.2000000000000002 1216 50 3.5999999999999996 1216 51 3.5999999999999996 2107 1 -3.5999999999999996 2107 2 1.2000000000000002 2107 3 3.6 2107 4 2.4000000000000004 2107 5 3.6 2107 6 NaN 2107 7 2.4000000000000004 2107 8 NaN 2107 9 NaN 2107 10 NaN 2107 11 NaN 2107 12 1.2000000000000002 2107 13 NaN 2107 14 2.4000000000000004 2107 15 NaN 2107 16 0.8000000000000007 2107 17 0.8000000000000007 2107 18 2.4000000000000004 2107 19 1.2000000000000002 2107 20 1.2000000000000002 2107 21 NaN 2107 22 NaN 2107 23 NaN 2107 24 NaN 2107 25 1.2000000000000002 2107 26 1.2000000000000002 2107 27 1.2000000000000002 2107 28 NaN 2107 29 NaN 2107 30 NaN 2107 31 NaN 2107 32 NaN 2107 33 1.2000000000000002 2107 34 1.2000000000000002 2107 35 NaN 2107 36 NaN 2107 37 NaN 2107 38 NaN 2107 39 NaN 2107 40 NaN 2107 41 2.4000000000000004 2107 42 2.4000000000000004 2107 43 0.8000000000000007 2107 44 NaN 2107 45 1.2000000000000002 2107 46 NaN 2107 47 NaN 2107 48 2.0 2107 49 1.2000000000000002 2107 50 3.5999999999999996 2107 51 3.5999999999999996 2210 1 2.4000000000000004 2210 2 1.2000000000000002 2210 3 -2.4 2210 4 2.4000000000000004 2210 5 -2.4 2210 6 NaN 2210 7 0.40000000000000036 2210 8 NaN 2210 9 NaN 2210 10 NaN 2210 11 NaN 2210 12 -0.7999999999999998 2210 13 NaN 2210 14 0.40000000000000036 2210 15 NaN 2210 16 0.8000000000000007 2210 17 0.8000000000000007 2210 18 0.40000000000000036 2210 19 -0.7999999999999998 2210 20 -0.7999999999999998 2210 21 NaN 2210 22 NaN 2210 23 NaN 2210 24 NaN 2210 25 -0.7999999999999998 2210 26 -0.7999999999999998 2210 27 -0.7999999999999998 2210 28 NaN 2210 29 NaN 2210 30 NaN 2210 31 NaN 2210 32 NaN 2210 33 -0.7999999999999998 2210 34 -0.7999999999999998 2210 35 NaN 2210 36 NaN 2210 37 NaN 2210 38 NaN 2210 39 NaN 2210 40 NaN 2210 41 0.40000000000000036 2210 42 0.40000000000000036 2210 43 0.8000000000000007 2210 44 NaN 2210 45 -0.7999999999999998 2210 46 NaN 2210 47 NaN 2210 48 0.0 2210 49 -0.7999999999999998 2210 50 1.5999999999999996 2210 51 1.5999999999999996 >>END_MODULE >>Per sequence quality scores pass #Quality Count 27 1.0 28 0.0 29 0.0 30 0.0 31 0.0 32 2.0 33 3.0 34 3.0 35 3.0 36 3.0 37 5.0 38 8.0 39 30.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 29.310344827586203 12.068965517241379 31.03448275862069 27.586206896551722 2 15.517241379310345 17.24137931034483 51.724137931034484 15.517241379310345 3 13.793103448275861 20.689655172413794 37.93103448275862 27.586206896551722 4 20.689655172413794 15.517241379310345 31.03448275862069 32.758620689655174 5 10.344827586206897 31.03448275862069 43.103448275862064 15.517241379310345 6 39.6551724137931 39.6551724137931 13.793103448275861 6.896551724137931 7 24.137931034482758 34.48275862068966 15.517241379310345 25.862068965517242 8 25.862068965517242 37.93103448275862 24.137931034482758 12.068965517241379 9 22.413793103448278 18.96551724137931 24.137931034482758 34.48275862068966 10 10.344827586206897 34.48275862068966 25.862068965517242 29.310344827586203 11 31.03448275862069 25.862068965517242 27.586206896551722 15.517241379310345 12 20.689655172413794 34.48275862068966 22.413793103448278 22.413793103448278 13 27.586206896551722 25.862068965517242 17.24137931034483 29.310344827586203 14 13.793103448275861 18.96551724137931 27.586206896551722 39.6551724137931 15 18.96551724137931 37.93103448275862 18.96551724137931 24.137931034482758 16 17.24137931034483 29.310344827586203 34.48275862068966 18.96551724137931 17 22.413793103448278 32.758620689655174 31.03448275862069 13.793103448275861 18 20.689655172413794 32.758620689655174 24.137931034482758 22.413793103448278 19 15.517241379310345 25.862068965517242 27.586206896551722 31.03448275862069 20 24.137931034482758 25.862068965517242 31.03448275862069 18.96551724137931 21 22.413793103448278 18.96551724137931 43.103448275862064 15.517241379310345 22 22.413793103448278 22.413793103448278 29.310344827586203 25.862068965517242 23 20.689655172413794 27.586206896551722 29.310344827586203 22.413793103448278 24 25.862068965517242 29.310344827586203 36.206896551724135 8.620689655172415 25 18.96551724137931 24.137931034482758 32.758620689655174 24.137931034482758 26 29.310344827586203 25.862068965517242 20.689655172413794 24.137931034482758 27 22.413793103448278 25.862068965517242 25.862068965517242 25.862068965517242 28 20.689655172413794 31.03448275862069 32.758620689655174 15.517241379310345 29 29.310344827586203 20.689655172413794 25.862068965517242 24.137931034482758 30 20.689655172413794 27.586206896551722 29.310344827586203 22.413793103448278 31 22.413793103448278 24.137931034482758 29.310344827586203 24.137931034482758 32 17.24137931034483 32.758620689655174 24.137931034482758 25.862068965517242 33 24.137931034482758 18.96551724137931 32.758620689655174 24.137931034482758 34 12.068965517241379 17.24137931034483 34.48275862068966 36.206896551724135 35 27.586206896551722 18.96551724137931 22.413793103448278 31.03448275862069 36 22.413793103448278 31.03448275862069 37.93103448275862 8.620689655172415 37 22.413793103448278 34.48275862068966 22.413793103448278 20.689655172413794 38 20.689655172413794 32.758620689655174 24.137931034482758 22.413793103448278 39 18.96551724137931 27.586206896551722 29.310344827586203 24.137931034482758 40 15.517241379310345 36.206896551724135 20.689655172413794 27.586206896551722 41 22.413793103448278 29.310344827586203 22.413793103448278 25.862068965517242 42 25.862068965517242 15.517241379310345 37.93103448275862 20.689655172413794 43 27.586206896551722 20.689655172413794 32.758620689655174 18.96551724137931 44 18.96551724137931 29.310344827586203 29.310344827586203 22.413793103448278 45 20.689655172413794 20.689655172413794 41.37931034482759 17.24137931034483 46 15.517241379310345 27.586206896551722 29.310344827586203 27.586206896551722 47 15.517241379310345 20.689655172413794 34.48275862068966 29.310344827586203 48 22.413793103448278 24.137931034482758 27.586206896551722 25.862068965517242 49 13.793103448275861 25.862068965517242 34.48275862068966 25.862068965517242 50 20.689655172413794 27.586206896551722 27.586206896551722 24.137931034482758 51 18.96551724137931 31.03448275862069 27.586206896551722 22.413793103448278 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 2.0 31 4.0 32 3.5 33 3.0 34 5.0 35 7.0 36 7.0 37 7.0 38 5.5 39 4.0 40 4.5 41 5.0 42 3.5 43 2.0 44 3.5 45 5.0 46 3.5 47 2.0 48 1.0 49 0.0 50 1.5 51 3.0 52 5.0 53 7.0 54 4.0 55 1.0 56 2.5 57 4.0 58 2.0 59 0.0 60 0.5 61 1.0 62 1.0 63 1.0 64 0.5 65 0.0 66 0.5 67 1.0 68 0.5 69 0.0 70 0.0 71 0.0 72 0.5 73 1.0 74 0.5 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 58.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 100.0 #Duplication Level Percentage of deduplicated Percentage of total 1 100.0 100.0 2 0.0 0.0 3 0.0 0.0 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TCTCTAATCCTAACAATTTTTCGGACCCCGTACTCCTGTCTCTTATACACA 1 1.7241379310344827 No Hit ACAGAATGATACAGCAGATGTCAATACTATCAGAGATACAAGAAATAGCAG 1 1.7241379310344827 No Hit GTATGGAATTGTGAAAGAGCTTGACGAGCCTCTAAACCTCAAGGACTGTCT 1 1.7241379310344827 No Hit GTCTGGACAATATGAACTCTTGCAGACGATAAACCTGTTATCCCTAGCGTA 1 1.7241379310344827 No Hit GGCCCAGTTCACCGCCGCCGGCGAGGAACTGCTCAACGAAGGCCGCCGCCT 1 1.7241379310344827 No Hit TGACTAGTATTTGTTTTTCAATTGGAAGTGGTGCATATTGCTGTCTCTTAT 1 1.7241379310344827 No Hit CGATCACCGCGTCACATACTTCCTCGGCGAACTCCAGTTCGGTTGCACTGA 1 1.7241379310344827 No Hit TTACCAAGAGATTCTTCAATCTTCTACAAATACTCAAACTAAATGCTGTCT 1 1.7241379310344827 No Hit GAGATTGTAACAGCATTTTTACTTTCTGTACAACAAAAAAGCTGTCTCTTA 1 1.7241379310344827 No Hit ATTCTCGGGTCATTACGCACAGCATTGTGTGCTGGGTCAACCAAGACTGTC 1 1.7241379310344827 No Hit ATTGTGAATCAGGTTGAATACTATTTCAGTGACTTGAACTTGGCTACTACA 1 1.7241379310344827 No Hit TCTTGGTACCTGGTATGTACTTCTTGGGGTTGAGCAAGTAATCGTACAAGG 1 1.7241379310344827 No Hit AATATGAATCACGTAAAGGTAGAAGAGAAGATGTGTGCAGCTGTTTGAATT 1 1.7241379310344827 No Hit ACAGAGGGAAGGATACAAATGGCTCTCAGTTCTTTATAACAACAAAACCAA 1 1.7241379310344827 No Hit CTTCCACGCCGGCCTCGGCCAGCTTCTTGCGAACTTCGGGCATGGCTAGTA 1 1.7241379310344827 No Hit CTTGACATACAGGGTGAGTATCGTCATTTCTACCAGCAACAGATCATAAAT 1 1.7241379310344827 No Hit CTGTCCGATCGGGCAATGGTGCGAAGCTATCATCTGCTGGATAATGGCTGA 1 1.7241379310344827 No Hit ATTCCATTCCATTCCATTCCGTTCTGTTCCATTCCATTCCATTCCATTCTA 1 1.7241379310344827 No Hit CTGTTGCCTGTACCCTTACTCATCTCCAATTCCTGTACTACTGAATCCCTC 1 1.7241379310344827 No Hit CTAGAGGGGTTCGTGAAGAGTACTTTCATCTAAACATCGAGGACTAAGATT 1 1.7241379310344827 No Hit GCACTTAGAAGGATGTCAAGCCCAGCGTTACAGCTGAGGTTGGAGACTTCT 1 1.7241379310344827 No Hit CACTATATACTCTAGTACAGGAATATCAACCTGTTGTCCATCTGTCTCTTA 1 1.7241379310344827 No Hit TTTTAATTCAAACCACTACAATGTACATGCATACCGCCATGTGTGTCTACA 1 1.7241379310344827 No Hit TACATGCACACACCCTTACAGTGACGAAGCACCCGTAATAATGTCGATAAG 1 1.7241379310344827 No Hit TTGTTGACCAGAACAGAGCTTTGGTGGATGCCCCTGATATGGAGAGGATCC 1 1.7241379310344827 No Hit TCTACGCGCTCCACCACTCCTTCCGGTATGGCTTCTCTGCTTGTAGAACGC 1 1.7241379310344827 No Hit GATCTTATGAGTACCGACACCCTGCCCCAACCCTCAAACCACGTACCGCGT 1 1.7241379310344827 No Hit GATAAACTTAGGTCATTGGTTAAGAAGTGGCAGACTTTGATCTGTCTCTTA 1 1.7241379310344827 No Hit GGGTTTCATGGAAAAGGTAATCTGCCATGATTTATACCTGTCTCTTATACA 1 1.7241379310344827 No Hit GGTATCAACGCAGAGTACGGGGAACAGGGACAACAGGCACATCAGGTTCAG 1 1.7241379310344827 No Hit TTTCAGAGCAGGAACAAGTTCTGATTCTTCAGTTCCTATAATACAATGAGG 1 1.7241379310344827 No Hit TGTGTGGACTGTCTGGTAGGTAAATCCGCTGGGCGTGAAAGTTGAGGCATG 1 1.7241379310344827 No Hit GTGGTAGATAGCAATCACGATTTAGTTGCCCAAGTTCATTTAGAATTTAAA 1 1.7241379310344827 No Hit GGACAAGATAAAATAGCTTATTTTGCTAAGGCTGATTAGACAGACGGTTGG 1 1.7241379310344827 No Hit GTGTTGTGGTAACCTTTTTGACTTAAGTTGGTTAAGAACTGTCTCTTATAC 1 1.7241379310344827 No Hit CCTTCATTGACCTCGACTACATGGTGTACATGCTCAAGTATGACTCTGTCT 1 1.7241379310344827 No Hit TCCCTTACCACACTCTACTATACTTCGAGTTCTGGTTAGCCTGTCTCTTAT 1 1.7241379310344827 No Hit GTCCATCGACTACGCCTGTCGGCCTCGCCTTAGGTCCCGACTCACCCAGGG 1 1.7241379310344827 No Hit CAAGGAGGTTTCCTTTCATGTAATAAAATGGCCCCAAGCAAACATTAATTG 1 1.7241379310344827 No Hit ATTCTGAAAGATAAGTACTGAAAGCATATCAAGTGCGAAACCTGTCTCTTA 1 1.7241379310344827 No Hit GTACGGGGGCTCCCACGATAGTGTATCTCAAGCACTGGAGCGAATTCAGGG 1 1.7241379310344827 No Hit CACCAACACCAGGCCTACCGTGCTTGACCGGCTTGTATGAGATAGAGAACT 1 1.7241379310344827 No Hit CTCTTGTACCTAACAATCTTTAATCGTGCTCTTTTTAATAAGAACTTCGTC 1 1.7241379310344827 No Hit CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC 1 1.7241379310344827 RNA PCR Primer, Index 23 (95% over 21bp) GGCATGCATCGAGCGTCGTGTGAGGACAGTAGATGTGGATGCCCGCTGCGA 1 1.7241379310344827 No Hit TTGTTTACCCATGGCTGACTGGTAAGTGTTTCGCGGCGACTGGTTGTGGTC 1 1.7241379310344827 No Hit TTCCTGAAGTTACCGTAAATGATAAAACTTATACCTGTCTCTTATACACAT 1 1.7241379310344827 No Hit CTTGCACAGTGCCTTAGACGAGTTCTTTGATATAATGGACAGCCGATGCAC 1 1.7241379310344827 No Hit CTCTAGTACAGGAATATCAACCTGTTGTCCATCGGATACACCTTTCGGTCT 1 1.7241379310344827 No Hit CTACAGATCAGAGAGTTTCCATTTACTTGCAGCAGCCCTCGGGCTTACCCG 1 1.7241379310344827 No Hit CTTTGACAGTCTCTAAGTCTTCAGCAGTAATTTCGAAATCAAGCTCCTTGC 1 1.7241379310344827 No Hit TTCCAGAGTATTGGAAATGATGAAGAAGGTAGGAGGACCTGTCTCTTATAC 1 1.7241379310344827 No Hit TGTTTAGTGATACTATGCCACATGCGATCATTTCAATCTGTCTCTTATACA 1 1.7241379310344827 No Hit TTCTAACGCTGCTCTCGAATTAATGTAATGTTGTTTATGCTTTTTATGTGA 1 1.7241379310344827 No Hit GATGTATCTTCAGTGCTATATCAACGAGAAGGGTGAGAAGGTTTACACAAC 1 1.7241379310344827 No Hit GTCGAACTGAATTAAGTGGAGTGTTCTCCACACTATATCGATAGTAGTCAT 1 1.7241379310344827 No Hit TACAAGAAGTAGAGAGACATTGAGATACTACGTTCTGTCTCTTATACACAT 1 1.7241379310344827 No Hit TCCGAAGACGTTCCACAAAGGCTGAGTTAGCTACCTGGTCCTTGGTCTCAC 1 1.7241379310344827 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.0 22 0.0 0.0 0.0 23 0.0 0.0 0.0 24 0.0 0.0 0.0 25 0.0 0.0 0.0 26 0.0 0.0 0.0 27 0.0 0.0 0.0 28 0.0 0.0 0.0 29 0.0 0.0 0.0 30 0.0 0.0 0.0 31 0.0 0.0 0.0 32 0.0 0.0 0.0 33 0.0 0.0 0.0 34 0.0 0.0 0.0 35 0.0 0.0 3.4482758620689653 36 0.0 0.0 5.172413793103448 37 0.0 0.0 5.172413793103448 38 0.0 0.0 8.620689655172415 39 0.0 0.0 12.068965517241379 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCTGGG 5 0.0 45.0 27 GAGGCAT 5 0.0 45.0 44 ACTGTCT 5 0.0 45.0 8 GTTGAGG 5 0.0 45.0 41 AATCCGC 5 0.0 45.0 23 TAAATCC 5 0.0 45.0 21 AAATCCG 5 0.0 45.0 22 TGGTAGG 5 0.0 45.0 14 TGTCTGG 5 0.0 45.0 10 TCTGGTA 5 0.0 45.0 12 GGCGTGA 5 0.0 45.0 32 CTGGGCG 5 0.0 45.0 29 GGACTGT 5 0.0 45.0 6 CCGCTGG 5 0.0 45.0 26 GCTGGGC 5 0.0 45.0 28 GTCTGGT 5 0.0 45.0 11 TTGAGGC 5 0.0 45.0 42 GCGTGAA 5 0.0 45.0 33 CTGGTAG 5 0.0 45.0 13 TGGACTG 5 0.0 45.0 5 >>END_MODULE