FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_4_88.3410000000b29f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_4_88.3410000000b29f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCTAATCCTAACAATTTTTCGGACCCCGTACTCCTGTCTCTTATACACA11.7241379310344827No Hit
ACAGAATGATACAGCAGATGTCAATACTATCAGAGATACAAGAAATAGCAG11.7241379310344827No Hit
GTATGGAATTGTGAAAGAGCTTGACGAGCCTCTAAACCTCAAGGACTGTCT11.7241379310344827No Hit
GTCTGGACAATATGAACTCTTGCAGACGATAAACCTGTTATCCCTAGCGTA11.7241379310344827No Hit
GGCCCAGTTCACCGCCGCCGGCGAGGAACTGCTCAACGAAGGCCGCCGCCT11.7241379310344827No Hit
TGACTAGTATTTGTTTTTCAATTGGAAGTGGTGCATATTGCTGTCTCTTAT11.7241379310344827No Hit
CGATCACCGCGTCACATACTTCCTCGGCGAACTCCAGTTCGGTTGCACTGA11.7241379310344827No Hit
TTACCAAGAGATTCTTCAATCTTCTACAAATACTCAAACTAAATGCTGTCT11.7241379310344827No Hit
GAGATTGTAACAGCATTTTTACTTTCTGTACAACAAAAAAGCTGTCTCTTA11.7241379310344827No Hit
ATTCTCGGGTCATTACGCACAGCATTGTGTGCTGGGTCAACCAAGACTGTC11.7241379310344827No Hit
ATTGTGAATCAGGTTGAATACTATTTCAGTGACTTGAACTTGGCTACTACA11.7241379310344827No Hit
TCTTGGTACCTGGTATGTACTTCTTGGGGTTGAGCAAGTAATCGTACAAGG11.7241379310344827No Hit
AATATGAATCACGTAAAGGTAGAAGAGAAGATGTGTGCAGCTGTTTGAATT11.7241379310344827No Hit
ACAGAGGGAAGGATACAAATGGCTCTCAGTTCTTTATAACAACAAAACCAA11.7241379310344827No Hit
CTTCCACGCCGGCCTCGGCCAGCTTCTTGCGAACTTCGGGCATGGCTAGTA11.7241379310344827No Hit
CTTGACATACAGGGTGAGTATCGTCATTTCTACCAGCAACAGATCATAAAT11.7241379310344827No Hit
CTGTCCGATCGGGCAATGGTGCGAAGCTATCATCTGCTGGATAATGGCTGA11.7241379310344827No Hit
ATTCCATTCCATTCCATTCCGTTCTGTTCCATTCCATTCCATTCCATTCTA11.7241379310344827No Hit
CTGTTGCCTGTACCCTTACTCATCTCCAATTCCTGTACTACTGAATCCCTC11.7241379310344827No Hit
CTAGAGGGGTTCGTGAAGAGTACTTTCATCTAAACATCGAGGACTAAGATT11.7241379310344827No Hit
GCACTTAGAAGGATGTCAAGCCCAGCGTTACAGCTGAGGTTGGAGACTTCT11.7241379310344827No Hit
CACTATATACTCTAGTACAGGAATATCAACCTGTTGTCCATCTGTCTCTTA11.7241379310344827No Hit
TTTTAATTCAAACCACTACAATGTACATGCATACCGCCATGTGTGTCTACA11.7241379310344827No Hit
TACATGCACACACCCTTACAGTGACGAAGCACCCGTAATAATGTCGATAAG11.7241379310344827No Hit
TTGTTGACCAGAACAGAGCTTTGGTGGATGCCCCTGATATGGAGAGGATCC11.7241379310344827No Hit
TCTACGCGCTCCACCACTCCTTCCGGTATGGCTTCTCTGCTTGTAGAACGC11.7241379310344827No Hit
GATCTTATGAGTACCGACACCCTGCCCCAACCCTCAAACCACGTACCGCGT11.7241379310344827No Hit
GATAAACTTAGGTCATTGGTTAAGAAGTGGCAGACTTTGATCTGTCTCTTA11.7241379310344827No Hit
GGGTTTCATGGAAAAGGTAATCTGCCATGATTTATACCTGTCTCTTATACA11.7241379310344827No Hit
GGTATCAACGCAGAGTACGGGGAACAGGGACAACAGGCACATCAGGTTCAG11.7241379310344827No Hit
TTTCAGAGCAGGAACAAGTTCTGATTCTTCAGTTCCTATAATACAATGAGG11.7241379310344827No Hit
TGTGTGGACTGTCTGGTAGGTAAATCCGCTGGGCGTGAAAGTTGAGGCATG11.7241379310344827No Hit
GTGGTAGATAGCAATCACGATTTAGTTGCCCAAGTTCATTTAGAATTTAAA11.7241379310344827No Hit
GGACAAGATAAAATAGCTTATTTTGCTAAGGCTGATTAGACAGACGGTTGG11.7241379310344827No Hit
GTGTTGTGGTAACCTTTTTGACTTAAGTTGGTTAAGAACTGTCTCTTATAC11.7241379310344827No Hit
CCTTCATTGACCTCGACTACATGGTGTACATGCTCAAGTATGACTCTGTCT11.7241379310344827No Hit
TCCCTTACCACACTCTACTATACTTCGAGTTCTGGTTAGCCTGTCTCTTAT11.7241379310344827No Hit
GTCCATCGACTACGCCTGTCGGCCTCGCCTTAGGTCCCGACTCACCCAGGG11.7241379310344827No Hit
CAAGGAGGTTTCCTTTCATGTAATAAAATGGCCCCAAGCAAACATTAATTG11.7241379310344827No Hit
ATTCTGAAAGATAAGTACTGAAAGCATATCAAGTGCGAAACCTGTCTCTTA11.7241379310344827No Hit
GTACGGGGGCTCCCACGATAGTGTATCTCAAGCACTGGAGCGAATTCAGGG11.7241379310344827No Hit
CACCAACACCAGGCCTACCGTGCTTGACCGGCTTGTATGAGATAGAGAACT11.7241379310344827No Hit
CTCTTGTACCTAACAATCTTTAATCGTGCTCTTTTTAATAAGAACTTCGTC11.7241379310344827No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC11.7241379310344827RNA PCR Primer, Index 23 (95% over 21bp)
GGCATGCATCGAGCGTCGTGTGAGGACAGTAGATGTGGATGCCCGCTGCGA11.7241379310344827No Hit
TTGTTTACCCATGGCTGACTGGTAAGTGTTTCGCGGCGACTGGTTGTGGTC11.7241379310344827No Hit
TTCCTGAAGTTACCGTAAATGATAAAACTTATACCTGTCTCTTATACACAT11.7241379310344827No Hit
CTTGCACAGTGCCTTAGACGAGTTCTTTGATATAATGGACAGCCGATGCAC11.7241379310344827No Hit
CTCTAGTACAGGAATATCAACCTGTTGTCCATCGGATACACCTTTCGGTCT11.7241379310344827No Hit
CTACAGATCAGAGAGTTTCCATTTACTTGCAGCAGCCCTCGGGCTTACCCG11.7241379310344827No Hit
CTTTGACAGTCTCTAAGTCTTCAGCAGTAATTTCGAAATCAAGCTCCTTGC11.7241379310344827No Hit
TTCCAGAGTATTGGAAATGATGAAGAAGGTAGGAGGACCTGTCTCTTATAC11.7241379310344827No Hit
TGTTTAGTGATACTATGCCACATGCGATCATTTCAATCTGTCTCTTATACA11.7241379310344827No Hit
TTCTAACGCTGCTCTCGAATTAATGTAATGTTGTTTATGCTTTTTATGTGA11.7241379310344827No Hit
GATGTATCTTCAGTGCTATATCAACGAGAAGGGTGAGAAGGTTTACACAAC11.7241379310344827No Hit
GTCGAACTGAATTAAGTGGAGTGTTCTCCACACTATATCGATAGTAGTCAT11.7241379310344827No Hit
TACAAGAAGTAGAGAGACATTGAGATACTACGTTCTGTCTCTTATACACAT11.7241379310344827No Hit
TCCGAAGACGTTCCACAAAGGCTGAGTTAGCTACCTGGTCCTTGGTCTCAC11.7241379310344827No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGGG50.045.027
GAGGCAT50.045.044
ACTGTCT50.045.08
GTTGAGG50.045.041
AATCCGC50.045.023
TAAATCC50.045.021
AAATCCG50.045.022
TGGTAGG50.045.014
TGTCTGG50.045.010
TCTGGTA50.045.012
GGCGTGA50.045.032
CTGGGCG50.045.029
GGACTGT50.045.06
CCGCTGG50.045.026
GCTGGGC50.045.028
GTCTGGT50.045.011
TTGAGGC50.045.042
GCGTGAA50.045.033
CTGGTAG50.045.013
TGGACTG50.045.05
Produced by FastQC (version 0.11.2)