FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_4_87.3410000000b282.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_4_87.3410000000b282.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93960
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAC250.002070619736.0939181
CGTCCAT250.002097904735.9978731
GTATCAA1100.030.7618621
GGTATCA602.4014026E-422.5586991
TATCAAC1851.4551915E-1118.2421652
ATCAACG2403.6379788E-1215.9365573
AACGCAG2151.4551915E-1015.6967456
TCAACGC2151.4551915E-1015.6967454
CGCAGAG2453.6379788E-1215.61132057
CAACGCA2304.0381565E-1014.6730465
GCAGAGT2355.5661076E-1014.3608539
TACGGGA950.005329656414.20968514
ACGCAGA2407.6397555E-1014.0616686
CAGAGTA2407.6397555E-1014.06166810
AGAGTAC2551.895387E-913.23451211
AGTACGG2451.547778E-812.85638112
GAGTACG2502.0527295E-812.59925611
GTACGGG2705.997026E-811.66597713
Produced by FastQC (version 0.11.2)