FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_4_86.3410000000b275.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_4_86.3410000000b275.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9619
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC240.24950618567418653RNA PCR Primer, Index 45 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA405.728723E-428.1250021
TATCAAC405.728723E-428.1250022
TCAACGC450.00114019524.9999984
CAGAGTA450.00114019524.99999810
AGAGTAC450.00114019524.99999811
GCAGAGT450.00114019524.9999989
ATCAACG450.00114019524.9999983
CAACGCA450.00114019524.9999985
AACGCAG500.002106358322.56
ACGCAGA500.002106358322.57
CGCAGAG500.002106358322.58
Produced by FastQC (version 0.11.2)