FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_4_85.3410000000b25c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_4_85.3410000000b25c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50566
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTC3100.6130601590001186No Hit
ACTTAGATGTTTCAGTTCGCTAAGTTTTCAAAGTCCAAAGAGCGCAGACTA950.18787327453229444No Hit
GCATTGGATGGATGCCCGGGCATTGAGAAGGAAGGACGCTTTCAGAGGCGA810.16018668670648262No Hit
ATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCTTC550.10876873788711783RNA PCR Primer, Index 37 (95% over 23bp)
GTATCAACGCAGAGTACGGGGAATGCATTGGATGGATGCCCGGGCATTGAG520.10283589763872958No Hit
GAATGCATTGGATGGATGCCCGGGCATTGAGAAGGAAGGACGCTTTCAGAG520.10283589763872958No Hit
TTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCT510.10085828422260017RNA PCR Primer, Index 44 (95% over 23bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGAA206.9716707E-444.99703635
CGAAATG300.005103026529.9980231
ACTTAGA405.9815205E-428.2068461
TACGGGA450.001210868424.99835215
GTATCAA1500.022.5654771
AACGCAG1651.8189894E-1220.4531976
TATCAAC1703.6379788E-1219.8516312
CGCAGAG1703.6379788E-1219.8516318
ACGCAGA1755.456968E-1219.2844437
ATCAACG1755.456968E-1219.2844433
AGAGTAC1755.456968E-1219.28444311
GCAGAGT1809.094947E-1218.7487649
CAGAGTA1809.094947E-1218.74876410
AGTACGG1607.548806E-1018.28004513
CAACGCA1902.1827873E-1117.7619865
GAGTACG1701.6952981E-917.20474812
TCAACGC2004.7293724E-1116.8738864
GGTATCA850.002471428615.9285711
GTACGGG1754.5583874E-815.42755414
ATACACA1000.007423885613.4991094
Produced by FastQC (version 0.11.2)