FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_4_83.3410000000b232.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_4_83.3410000000b232.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences323699
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC20440.6314508231412516No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA12820.3960469448469102No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTCT12040.3719504848640249No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11050.34136651642420895No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT10560.3262289966913707No Hit
GTATCAACGCAGAGTACGGGGCGACCCCAGGTCAGGCGGGATTACCCGCTG10540.32561113874309155No Hit
CCCTTAGTAACGGCGAGCGAACCGGGAAGAGCCCAGCTTGAAAATCGGACG8560.26444320186345954No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTC8530.2635164149410409No Hit
GTACTTGTTCGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAATTT6860.21192527625973515No Hit
CCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAGCGGA6530.20173062011312978No Hit
TAGTAACGGCGAGCGAACCGGGAAGAGCCCAGCTTGAAAATCGGACGTCTT6440.19895025934587382No Hit
TATCAACGCAGAGTACGGGGCGACCCCAGGTCAGGCGGGATTACCCGCTGA6050.18690202935443112No Hit
TATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAAAC5820.17979666294922134No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5770.17825201807852356No Hit
TTCCCAAACAACCCGACTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCG5660.17485379936298845No Hit
GTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAAA5270.16280556937154578No Hit
GGCCTAAGTTCCCTGGAAAGGGGCGCCAGAGAGGGTGAGAGCCCCGTCGTG5110.1578627057853129No Hit
GGATTACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAA5060.15631806091461511No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4900.15137519732838223No Hit
ACTTAGGCCCGGTCCGTCGCTGAGGACGCTTCTCCAGACTACAATTCGAAC4730.14612340476800978No Hit
GAATTTACCGCCCGATTGGGGCTGCATTCCCAAACAACCCGACTCGTAGAC4660.14396090194903288No Hit
TACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTA4640.1433430440007538No Hit
GAGTACGGGGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCAT4590.141798399130056No Hit
CTATCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAATTTACCGCCCGATT4590.141798399130056No Hit
TCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAG4290.13253052990586933No Hit
GTACGGGGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATAT4150.12820552426791557No Hit
GGTATCAACGCAGAGTACGGGGCGACCCCAGGTCAGGCGGGATTACCCGCT4030.1244983765782409No Hit
TCTTTACCTCGCGGTACTTGTTCGCTATCGGTCTCTCGCCCGTATTTAGCC3980.12295373170754312No Hit
GTCCGGGCACGACGGGGCTCTCACCCTCTCTGGCGCCCCTTTCCAGGGAAC3740.11553943632819379No Hit
GTCTGGAGAAGCGTCCTCAGCGACGGACCGGGCCTAAGTTCCCTGGAAAGG3700.11430372043163557No Hit
TCTCCAGACTACAATTCGAACGCCGAAGACGTCCGATTTTCAAGCTGGGCT3550.10966978581954223No Hit
CTCTGGCGCCCCTTTCCAGGGAACTTAGGCCCGGTCCGTCGCTGAGGACGC3540.10936085684540267No Hit
CTGGAGAAGCGTCCTCAGCGACGGACCGGGCCTAAGTTCCCTGGAAAGGGG3470.10719835402642579No Hit
CCGTTACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATG3420.105653709155728No Hit
CCCGTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCC3400.10503585120744889No Hit
CTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGGAAGAGCCCAGCTT3360.10380013531089066No Hit
CTACAATTCGAACGCCGAAGACGTCCGATTTTCAAGCTGGGCTCTTCCCGG3330.10287334838847201No Hit
GTGCGACAGGGTCCGGGCACGACGGGGCTCTCACCCTCTCTGGCGCCCCTT3280.10132870351777423No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC2650.040.752942
ACTAAGC2700.039.16491727
ATGCCGT2700.039.16491744
TCGTATG2650.039.0548640
GATCTCG2600.038.9405736
CGTATGC2800.038.5697141
GCCCACG2750.038.4528318
CTCGTAT2700.038.33162339
TCCGAGC2800.037.76617413
CACGAGA2800.037.76617421
TATGCCG2800.037.76617443
AGCCCAC2900.036.4638917
CCCACGA2900.036.4638919
CGAGACT2900.036.4638923
GAGACTA2950.035.8458624
TCTCGTA2900.035.68806538
CTCCGAG2950.035.0831812
CCGAGCC3050.034.6705814
TGCCGTC3050.034.6705845
TGATCTC3000.034.4984635
Produced by FastQC (version 0.11.2)