FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_4_81.3410000000b218.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_4_81.3410000000b218.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences968118
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTC24900.2572000520597696No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC2650.038.20623445
ACGAGAC2700.037.49870722
CGTATGC2800.036.1594741
TCGTATG2800.036.1594740
CGAGACT2800.036.1594723
TACTCGT250.00210730135.9987610
TATTTCG702.1827873E-1135.35592730
GATCTCG2800.035.35592736
CGCTATG2700.034.9987930
GCCCACG2900.034.9125918
TCTCGTA2900.034.9125938
CGAGCCC2900.034.9125915
GACTCGC2850.034.73564526
TATGCCG2950.034.32085443
CCGAGCC3100.034.1117314
CACGAGA3000.033.74883721
TCGCTAT2800.033.74883729
ACTCGCT2950.033.5581727
ATCTCGT3050.033.19557637
AGACTCG3000.032.99886325
Produced by FastQC (version 0.11.2)