Basic Statistics
Measure | Value |
---|---|
Filename | H23V3BCXX l02n01 kb2_4_80.3410000000b20b.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 707810 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 40 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC | 13555 | 1.9150619516536924 | RNA PCR Primer, Index 39 (95% over 21bp) |
TCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGT | 4387 | 0.6197990986281628 | No Hit |
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC | 3466 | 0.4896794337463444 | No Hit |
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA | 1367 | 0.1931309249657394 | No Hit |
AAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA | 1340 | 0.1893163419561747 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCG | 1320 | 0.18649072491205268 | No Hit |
GTCATAAGACAGGTATCGTAAATTTATTTACGAGGCAAACCGGGAGAAGTG | 859 | 0.12136025204504033 | No Hit |
AAAAAGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAGCCCACG | 746 | 0.10539551574575098 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGCCGTC | 1470 | 0.0 | 43.008583 | 45 |
ATGCCGT | 1890 | 0.0 | 33.57016 | 44 |
CTCGTAT | 1995 | 0.0 | 31.916088 | 39 |
GTTATAC | 2005 | 0.0 | 31.869125 | 30 |
TCGTATG | 2005 | 0.0 | 31.756908 | 40 |
CATCTCG | 2005 | 0.0 | 31.644693 | 36 |
TATGCCG | 2015 | 0.0 | 31.599308 | 43 |
CGAGACT | 2035 | 0.0 | 31.509869 | 23 |
ACGAGAC | 2050 | 0.0 | 31.389063 | 22 |
ATCTCGT | 2035 | 0.0 | 31.178186 | 37 |
CGTATGC | 2040 | 0.0 | 31.10177 | 41 |
CACGAGA | 2075 | 0.0 | 31.010881 | 21 |
CCACGAG | 2085 | 0.0 | 30.862148 | 20 |
TCTCGTA | 2100 | 0.0 | 30.213148 | 38 |
CCTCAGT | 30 | 0.0051468853 | 29.998873 | 22 |
AGAGCGC | 30 | 0.0051468853 | 29.998873 | 39 |
ACTGTTA | 2140 | 0.0 | 29.963825 | 27 |
TGTTATA | 2165 | 0.0 | 29.825668 | 29 |
GTATGCC | 2165 | 0.0 | 29.5139 | 42 |
GACTGTT | 2195 | 0.0 | 29.213022 | 26 |