FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_4_80.3410000000b20b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_4_80.3410000000b20b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences707810
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC135551.9150619516536924RNA PCR Primer, Index 39 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGT43870.6197990986281628No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC34660.4896794337463444No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA13670.1931309249657394No Hit
AAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA13400.1893163419561747No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCG13200.18649072491205268No Hit
GTCATAAGACAGGTATCGTAAATTTATTTACGAGGCAAACCGGGAGAAGTG8590.12136025204504033No Hit
AAAAAGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAGCCCACG7460.10539551574575098No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC14700.043.00858345
ATGCCGT18900.033.5701644
CTCGTAT19950.031.91608839
GTTATAC20050.031.86912530
TCGTATG20050.031.75690840
CATCTCG20050.031.64469336
TATGCCG20150.031.59930843
CGAGACT20350.031.50986923
ACGAGAC20500.031.38906322
ATCTCGT20350.031.17818637
CGTATGC20400.031.1017741
CACGAGA20750.031.01088121
CCACGAG20850.030.86214820
TCTCGTA21000.030.21314838
CCTCAGT300.005146885329.99887322
AGAGCGC300.005146885329.99887339
ACTGTTA21400.029.96382527
TGTTATA21650.029.82566829
GTATGCC21650.029.513942
GACTGTT21950.029.21302226