##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23V3BCXX l02n01 kb2_4_8.3410000000ad04.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 40 Sequences flagged as poor quality 0 Sequence length 51 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.225 0.0 0.0 0.0 0.0 0.0 2 35.6 0.0 0.0 0.0 0.0 0.0 3 36.95 0.0 0.0 0.0 0.0 0.0 4 36.65 0.0 0.0 0.0 0.0 0.0 5 36.175 0.0 0.0 0.0 0.0 0.0 6 38.225 0.0 0.0 0.0 0.0 0.0 7 37.6 0.0 0.0 0.0 0.0 0.0 8 37.25 0.0 0.0 0.0 0.0 0.0 9 37.25 0.0 0.0 0.0 0.0 0.0 10 35.9 0.0 0.0 0.0 0.0 0.0 11 37.7 0.0 0.0 0.0 0.0 0.0 12 37.875 0.0 0.0 0.0 0.0 0.0 13 36.6 0.0 0.0 0.0 0.0 0.0 14 37.175 0.0 0.0 0.0 0.0 0.0 15 37.85 0.0 0.0 0.0 0.0 0.0 16 37.25 0.0 0.0 0.0 0.0 0.0 17 37.875 0.0 0.0 0.0 0.0 0.0 18 37.775 0.0 0.0 0.0 0.0 0.0 19 36.9 0.0 0.0 0.0 0.0 0.0 20 36.7 0.0 0.0 0.0 0.0 0.0 21 36.9 0.0 0.0 0.0 0.0 0.0 22 37.0 0.0 0.0 0.0 0.0 0.0 23 34.65 0.0 0.0 0.0 0.0 0.0 24 36.75 0.0 0.0 0.0 0.0 0.0 25 36.825 0.0 0.0 0.0 0.0 0.0 26 38.025 0.0 0.0 0.0 0.0 0.0 27 37.1 0.0 0.0 0.0 0.0 0.0 28 37.4 0.0 0.0 0.0 0.0 0.0 29 37.9 0.0 0.0 0.0 0.0 0.0 30 37.45 0.0 0.0 0.0 0.0 0.0 31 35.4 0.0 0.0 0.0 0.0 0.0 32 37.25 0.0 0.0 0.0 0.0 0.0 33 37.5 0.0 0.0 0.0 0.0 0.0 34 38.6 0.0 0.0 0.0 0.0 0.0 35 37.825 0.0 0.0 0.0 0.0 0.0 36 38.35 0.0 0.0 0.0 0.0 0.0 37 38.5 0.0 0.0 0.0 0.0 0.0 38 37.625 0.0 0.0 0.0 0.0 0.0 39 37.825 0.0 0.0 0.0 0.0 0.0 40 37.35 0.0 0.0 0.0 0.0 0.0 41 36.825 0.0 0.0 0.0 0.0 0.0 42 37.075 0.0 0.0 0.0 0.0 0.0 43 37.225 0.0 0.0 0.0 0.0 0.0 44 37.4 0.0 0.0 0.0 0.0 0.0 45 37.225 0.0 0.0 0.0 0.0 0.0 46 36.625 0.0 0.0 0.0 0.0 0.0 47 35.95 0.0 0.0 0.0 0.0 0.0 48 36.65 0.0 0.0 0.0 0.0 0.0 49 36.1 0.0 0.0 0.0 0.0 0.0 50 36.5 0.0 0.0 0.0 0.0 0.0 51 36.375 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2102 1 NaN 2102 2 -3.0 2102 3 1.5 2102 4 0.0 2102 5 0.0 2102 6 1.0 2102 7 -1.0 2102 8 -1.0 2102 9 1.0 2102 10 NaN 2102 11 2.0 2102 12 0.5 2102 13 NaN 2102 14 1.0 2102 15 NaN 2102 16 NaN 2102 17 -1.0 2102 18 0.5 2102 19 2.5 2102 20 1.0 2102 21 1.0 2102 22 NaN 2102 23 NaN 2102 24 -1.0 2102 25 -0.5 2102 26 -1.0 2102 27 0.5 2102 28 2.5 2102 29 -0.5 2102 30 -0.5 2102 31 NaN 2102 32 -0.5 2102 33 0.5 2102 34 -1.0 2102 35 1.0 2102 36 1.0 2102 37 0.5 2102 38 -1.0 2102 39 -0.5 2102 40 -5.0 2102 41 NaN 2102 42 -0.5 2102 43 -1.0 2102 44 -1.0 2102 45 0.5 2102 46 0.5 2102 47 NaN 2102 48 NaN 2102 49 NaN 2102 50 0.5 2102 51 NaN 1115 1 NaN 1115 2 3.0 1115 3 1.5 1115 4 0.0 1115 5 0.0 1115 6 3.0 1115 7 1.0 1115 8 1.0 1115 9 3.0 1115 10 NaN 1115 11 2.0 1115 12 0.5 1115 13 NaN 1115 14 -1.0 1115 15 NaN 1115 16 NaN 1115 17 1.0 1115 18 0.5 1115 19 4.5 1115 20 3.0 1115 21 3.0 1115 22 NaN 1115 23 NaN 1115 24 1.0 1115 25 1.5 1115 26 1.0 1115 27 2.5 1115 28 2.5 1115 29 1.5 1115 30 1.5 1115 31 NaN 1115 32 1.5 1115 33 0.5 1115 34 1.0 1115 35 1.0 1115 36 1.0 1115 37 0.5 1115 38 1.0 1115 39 1.5 1115 40 3.0 1115 41 NaN 1115 42 1.5 1115 43 1.0 1115 44 1.0 1115 45 0.5 1115 46 2.5 1115 47 NaN 1115 48 NaN 1115 49 NaN 1115 50 0.5 1115 51 NaN 2115 1 NaN 2115 2 3.0 2115 3 -4.5 2115 4 0.0 2115 5 0.0 2115 6 -5.0 2115 7 -1.0 2115 8 -1.0 2115 9 -5.0 2115 10 NaN 2115 11 -6.0 2115 12 -1.5 2115 13 NaN 2115 14 -1.0 2115 15 NaN 2115 16 NaN 2115 17 -1.0 2115 18 -1.5 2115 19 -3.5 2115 20 -5.0 2115 21 -5.0 2115 22 NaN 2115 23 NaN 2115 24 -1.0 2115 25 -0.5 2115 26 -1.0 2115 27 -5.5 2115 28 -5.5 2115 29 -0.5 2115 30 -0.5 2115 31 NaN 2115 32 -0.5 2115 33 -1.5 2115 34 -1.0 2115 35 -1.0 2115 36 -1.0 2115 37 0.5 2115 38 1.0 2115 39 -0.5 2115 40 1.0 2115 41 NaN 2115 42 -0.5 2115 43 -1.0 2115 44 -1.0 2115 45 -1.5 2115 46 -5.5 2115 47 NaN 2115 48 NaN 2115 49 NaN 2115 50 -1.5 2115 51 NaN 2215 1 NaN 2215 2 -3.0 2215 3 1.5 2215 4 0.0 2215 5 0.0 2215 6 1.0 2215 7 1.0 2215 8 1.0 2215 9 1.0 2215 10 NaN 2215 11 2.0 2215 12 0.5 2215 13 NaN 2215 14 1.0 2215 15 NaN 2215 16 NaN 2215 17 1.0 2215 18 0.5 2215 19 -3.5 2215 20 1.0 2215 21 1.0 2215 22 NaN 2215 23 NaN 2215 24 1.0 2215 25 -0.5 2215 26 1.0 2215 27 2.5 2215 28 0.5 2215 29 -0.5 2215 30 -0.5 2215 31 NaN 2215 32 -0.5 2215 33 0.5 2215 34 1.0 2215 35 -1.0 2215 36 -1.0 2215 37 -1.5 2215 38 -1.0 2215 39 -0.5 2215 40 1.0 2215 41 NaN 2215 42 -0.5 2215 43 1.0 2215 44 1.0 2215 45 0.5 2215 46 2.5 2215 47 NaN 2215 48 NaN 2215 49 NaN 2215 50 0.5 2215 51 NaN >>END_MODULE >>Per sequence quality scores pass #Quality Count 28 2.0 29 0.0 30 0.0 31 1.0 32 3.0 33 0.0 34 1.0 35 2.0 36 4.0 37 6.0 38 10.0 39 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.0 27.500000000000004 15.0 17.5 2 5.0 27.500000000000004 47.5 20.0 3 12.5 22.5 47.5 17.5 4 5.0 17.5 55.00000000000001 22.5 5 15.0 37.5 32.5 15.0 6 20.0 47.5 20.0 12.5 7 20.0 22.5 30.0 27.500000000000004 8 17.5 35.0 25.0 22.5 9 27.500000000000004 15.0 17.5 40.0 10 7.5 40.0 30.0 22.5 11 27.500000000000004 32.5 30.0 10.0 12 12.5 30.0 35.0 22.5 13 22.5 27.500000000000004 37.5 12.5 14 22.5 17.5 37.5 22.5 15 17.5 27.500000000000004 37.5 17.5 16 17.5 30.0 30.0 22.5 17 20.0 35.0 17.5 27.500000000000004 18 32.5 25.0 25.0 17.5 19 20.0 20.0 47.5 12.5 20 27.500000000000004 35.0 25.0 12.5 21 17.5 27.500000000000004 30.0 25.0 22 20.0 30.0 30.0 20.0 23 17.5 25.0 37.5 20.0 24 20.0 37.5 32.5 10.0 25 25.0 22.5 30.0 22.5 26 30.0 20.0 35.0 15.0 27 7.5 50.0 25.0 17.5 28 20.0 37.5 32.5 10.0 29 20.0 32.5 27.500000000000004 20.0 30 12.5 37.5 30.0 20.0 31 12.5 35.0 35.0 17.5 32 20.0 32.5 37.5 10.0 33 20.0 30.0 25.0 25.0 34 17.5 30.0 35.0 17.5 35 10.0 35.0 35.0 20.0 36 27.500000000000004 20.0 45.0 7.5 37 15.0 27.500000000000004 27.500000000000004 30.0 38 20.0 20.0 32.5 27.500000000000004 39 7.5 22.5 42.5 27.500000000000004 40 22.5 32.5 27.500000000000004 17.5 41 20.0 32.5 30.0 17.5 42 17.5 35.0 22.5 25.0 43 15.0 22.5 35.0 27.500000000000004 44 22.5 35.0 27.500000000000004 15.0 45 12.5 27.500000000000004 40.0 20.0 46 15.0 32.5 30.0 22.5 47 17.5 25.0 37.5 20.0 48 17.5 25.0 20.0 37.5 49 12.5 37.5 30.0 20.0 50 12.5 20.0 35.0 32.5 51 22.5 15.0 30.0 32.5 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.5 24 1.0 25 0.5 26 0.5 27 1.0 28 1.5 29 2.0 30 2.5 31 3.0 32 3.5 33 4.0 34 4.0 35 4.0 36 5.0 37 6.0 38 5.5 39 5.0 40 4.0 41 3.0 42 3.5 43 4.0 44 2.5 45 1.0 46 1.0 47 1.0 48 1.5 49 2.0 50 2.0 51 2.0 52 1.0 53 0.0 54 0.0 55 0.0 56 0.0 57 0.0 58 0.0 59 0.0 60 0.0 61 0.0 62 0.0 63 0.0 64 0.0 65 0.0 66 0.0 67 0.0 68 0.5 69 1.0 70 0.5 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 40.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 100.0 #Duplication Level Percentage of deduplicated Percentage of total 1 100.0 100.0 2 0.0 0.0 3 0.0 0.0 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAAGGAACTCGACAAAAATACCTCCGTAAGTTCTGTCTCTTATACACATCT 1 2.5 No Hit ATTTTGTCTCTCTAGTAATATCATGTTTGCCTGATTATTCTTGATCTTTGT 1 2.5 No Hit GAGCAAATATGAAGAAGATGTTCATGCTCACAATCACACAAGTGTCTGTCT 1 2.5 No Hit ATCTTCTCCATTGATGTTTCCATCTAAACAATCTTTCCCAATGTTATCCAC 1 2.5 No Hit ATTTAGAGGAGTGTTTGGTTCTTTGGATAACAATATCCCAAACTGAAAATG 1 2.5 No Hit GCTTTACACATTGAATTCGAGACTTTGGTTGTGTATTATGACCTGTCTCTT 1 2.5 No Hit CTTTATCTGTGGGCACTATGAGGGTTATGATGAGCGTATTAAGACCTGTCT 1 2.5 No Hit GATCCTTACACTTTCATGAAAACTCCATAAATGACTGTCTCTTATACACAT 1 2.5 No Hit TATCAACGCAGAGTACGGGGCTCGCCGGCCGAGGTGGGATCCCGAGGCCTC 1 2.5 No Hit ATGTTGCGTTCCTCAAATGCAAGAAGAATGATCCAAACCCAGAGAAGTGTC 1 2.5 No Hit TCTCTGCTCAAAGGTCATTTCCTGGACTAATTTTTTCATGCAAGTGTCACA 1 2.5 No Hit CTGTAAGCGAACTCTGCGTAAGATCTTTCTTCATCGCGTCTCACTATATCT 1 2.5 No Hit GTATCAACGCAGAGTACGGGCAGTGGTATCAACCTGTCTCTTATACACATC 1 2.5 No Hit ATCTAGTACTTTCATTCCCATTTGATTTTCCTTGGACATATTAAAGCCTGT 1 2.5 No Hit ATCATAGCCAAAATGGAGTGTTAAGGAGAGAGTAGTACAGCATATATGAGA 1 2.5 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGG 1 2.5 No Hit CATCTATTATGATTTGACGATTGACCATTATCCCGGAACCTGTCTCTTATA 1 2.5 No Hit TATCCAGGGAGCTTGAAGTTCAACAGAGAATATAAATGCTGTCTCTTATAC 1 2.5 No Hit GAGCAAAATTTCTTTACTTATCGGGATTGTAGCTATCCTGTACCAATTACG 1 2.5 No Hit GTCTTCCTCTACATTACCTTAACAGTAATGAGTGACAGGCATTACCTCTGT 1 2.5 No Hit GTTTAGCATTATCTCTATTTGGGATTGTTTTTTGTTTTTGTCTTAGACTTT 1 2.5 No Hit TATCAACGCAGAGTACGGGGAAAATGTAGAAACTATCTAACAGAAACCAAG 1 2.5 No Hit ATCAATATCAGTATGTGCTCAATTATCAGACATTATTCAAGCTGTCTCTTA 1 2.5 No Hit GCAGGAATGAATTCTCGGCGTCAAGAAAACTGCCATCGACAGCGCTGAATC 1 2.5 No Hit GTTTTCGACCGGCACCTTGACGTTGTCGAAGAAGACTTCGTTCACTTCATG 1 2.5 No Hit GATAACTAGAATTCATAAAAGGTTCGTACTTGATAAAGATGCAAGGATCCC 1 2.5 No Hit ATATGCTGACCTTTGGCAATTTGAATTGACAACAAAGACAAATGTAAACCT 1 2.5 No Hit ACTAATGACACAATATCAAAAACATCCCATAAAATCTGAAAAGGGTAAAAC 1 2.5 No Hit CCTTTTGCATAATGAGCCTACGAGTTAATTTTACTAGCGAGGTTAAGGACC 1 2.5 No Hit TTATCATGATTTACGTTGTTGCTTATCTATGTTGTTGCGTACAAGTAAATC 1 2.5 No Hit GGTAAGTAACTTTTCCAAAGTTTTCGAGAATGACAGGGAAAAAGATGGGAT 1 2.5 No Hit CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC 1 2.5 No Hit GAATGTGTTGAAACTCCTCATTTGCAACCAGAGACATTTTGATTCTGTCTC 1 2.5 No Hit ACTCAGGTGGTTAGGTAGAGAATACTAAGGCTGTCTCTTATACACATCTCC 1 2.5 No Hit ATCAGATACCGTCGTAGTCTACCATAAACTATGCCGACTAGGGATCGGACG 1 2.5 No Hit CCTTGTTAGTAATGCCGCAAGGTTGAAAGCAAAAAGATTAGACCATCCCTA 1 2.5 No Hit GCCTTATACGTCGTATAATTGGAATGAGTACAATTTAAATCCCTTAACGAG 1 2.5 No Hit GTATCAACGCAGAGTACGGGATTATCACCCTCTATGATTGACCTTTCCAAG 1 2.5 No Hit CAATAACATATCGATATTACGCAAACCAATATAACGTCTGTCTCTTATACA 1 2.5 No Hit TGGTTTCTCTTATCCTAATGTTACAAAAGTTCGATTCTTGGAACACAATCG 1 2.5 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.0 22 0.0 0.0 0.0 23 0.0 0.0 0.0 24 0.0 0.0 0.0 25 0.0 0.0 0.0 26 0.0 0.0 0.0 27 0.0 0.0 0.0 28 0.0 0.0 0.0 29 0.0 0.0 0.0 30 0.0 0.0 0.0 31 0.0 0.0 2.5 32 0.0 0.0 2.5 33 0.0 0.0 5.0 34 0.0 0.0 7.5 35 0.0 0.0 10.0 36 0.0 0.0 10.0 37 0.0 0.0 10.0 38 0.0 0.0 12.5 39 0.0 0.0 15.0 >>END_MODULE >>Kmer Content pass >>END_MODULE