FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_4_8.3410000000ad04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_4_8.3410000000ad04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40
Sequences flagged as poor quality0
Sequence length51
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGGAACTCGACAAAAATACCTCCGTAAGTTCTGTCTCTTATACACATCT12.5No Hit
ATTTTGTCTCTCTAGTAATATCATGTTTGCCTGATTATTCTTGATCTTTGT12.5No Hit
GAGCAAATATGAAGAAGATGTTCATGCTCACAATCACACAAGTGTCTGTCT12.5No Hit
ATCTTCTCCATTGATGTTTCCATCTAAACAATCTTTCCCAATGTTATCCAC12.5No Hit
ATTTAGAGGAGTGTTTGGTTCTTTGGATAACAATATCCCAAACTGAAAATG12.5No Hit
GCTTTACACATTGAATTCGAGACTTTGGTTGTGTATTATGACCTGTCTCTT12.5No Hit
CTTTATCTGTGGGCACTATGAGGGTTATGATGAGCGTATTAAGACCTGTCT12.5No Hit
GATCCTTACACTTTCATGAAAACTCCATAAATGACTGTCTCTTATACACAT12.5No Hit
TATCAACGCAGAGTACGGGGCTCGCCGGCCGAGGTGGGATCCCGAGGCCTC12.5No Hit
ATGTTGCGTTCCTCAAATGCAAGAAGAATGATCCAAACCCAGAGAAGTGTC12.5No Hit
TCTCTGCTCAAAGGTCATTTCCTGGACTAATTTTTTCATGCAAGTGTCACA12.5No Hit
CTGTAAGCGAACTCTGCGTAAGATCTTTCTTCATCGCGTCTCACTATATCT12.5No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACCTGTCTCTTATACACATC12.5No Hit
ATCTAGTACTTTCATTCCCATTTGATTTTCCTTGGACATATTAAAGCCTGT12.5No Hit
ATCATAGCCAAAATGGAGTGTTAAGGAGAGAGTAGTACAGCATATATGAGA12.5No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGG12.5No Hit
CATCTATTATGATTTGACGATTGACCATTATCCCGGAACCTGTCTCTTATA12.5No Hit
TATCCAGGGAGCTTGAAGTTCAACAGAGAATATAAATGCTGTCTCTTATAC12.5No Hit
GAGCAAAATTTCTTTACTTATCGGGATTGTAGCTATCCTGTACCAATTACG12.5No Hit
GTCTTCCTCTACATTACCTTAACAGTAATGAGTGACAGGCATTACCTCTGT12.5No Hit
GTTTAGCATTATCTCTATTTGGGATTGTTTTTTGTTTTTGTCTTAGACTTT12.5No Hit
TATCAACGCAGAGTACGGGGAAAATGTAGAAACTATCTAACAGAAACCAAG12.5No Hit
ATCAATATCAGTATGTGCTCAATTATCAGACATTATTCAAGCTGTCTCTTA12.5No Hit
GCAGGAATGAATTCTCGGCGTCAAGAAAACTGCCATCGACAGCGCTGAATC12.5No Hit
GTTTTCGACCGGCACCTTGACGTTGTCGAAGAAGACTTCGTTCACTTCATG12.5No Hit
GATAACTAGAATTCATAAAAGGTTCGTACTTGATAAAGATGCAAGGATCCC12.5No Hit
ATATGCTGACCTTTGGCAATTTGAATTGACAACAAAGACAAATGTAAACCT12.5No Hit
ACTAATGACACAATATCAAAAACATCCCATAAAATCTGAAAAGGGTAAAAC12.5No Hit
CCTTTTGCATAATGAGCCTACGAGTTAATTTTACTAGCGAGGTTAAGGACC12.5No Hit
TTATCATGATTTACGTTGTTGCTTATCTATGTTGTTGCGTACAAGTAAATC12.5No Hit
GGTAAGTAACTTTTCCAAAGTTTTCGAGAATGACAGGGAAAAAGATGGGAT12.5No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC12.5No Hit
GAATGTGTTGAAACTCCTCATTTGCAACCAGAGACATTTTGATTCTGTCTC12.5No Hit
ACTCAGGTGGTTAGGTAGAGAATACTAAGGCTGTCTCTTATACACATCTCC12.5No Hit
ATCAGATACCGTCGTAGTCTACCATAAACTATGCCGACTAGGGATCGGACG12.5No Hit
CCTTGTTAGTAATGCCGCAAGGTTGAAAGCAAAAAGATTAGACCATCCCTA12.5No Hit
GCCTTATACGTCGTATAATTGGAATGAGTACAATTTAAATCCCTTAACGAG12.5No Hit
GTATCAACGCAGAGTACGGGATTATCACCCTCTATGATTGACCTTTCCAAG12.5No Hit
CAATAACATATCGATATTACGCAAACCAATATAACGTCTGTCTCTTATACA12.5No Hit
TGGTTTCTCTTATCCTAATGTTACAAAAGTTCGATTCTTGGAACACAATCG12.5No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers