FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_4_79.3410000000b1fd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_4_79.3410000000b1fd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1205019
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA19650.1630679682229077No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT18460.15319260526182574No Hit
TACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTA14820.12298561267498687No Hit
CTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTC13060.10838003384179004No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACG2150.034.5336118
TGCCGTC2350.031.5945845
CCCACGA2400.030.93635719
ACTACCG2300.030.32496827
GACTACC2300.030.32496826
CGAGCCC2500.028.79893915
TATGCCG2600.028.55663943
GTATGCC2600.028.55663942
CGAGACT2600.028.55663923
ATGCCGT2600.028.55663944
TCGCGTT406.1676494E-428.12396229
CCGAGCC2650.028.01783414
TATACCG654.875292E-727.6912865
CGTATGC2700.027.49898541
GGACCTA507.160481E-526.9990046
TCCGAGC2700.026.66568413
CGCTGAT2700.026.66568432
TCGTATG2800.026.5168840
CCACGAG2800.026.5168820
CTACCGC2650.026.31978228
Produced by FastQC (version 0.11.2)