FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_4_78.3410000000b1e0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_4_78.3410000000b1e0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences491916
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTC10930.22219240683368705RNA PCR Primer, Index 20 (95% over 21bp)
GATATGTACTCACTGATAAGTGGATATTAGCTCAGAAACCTAGAATACCCA8330.1693378544304312No Hit
CTATAACTCTTTCCATGGGATTTTGTTTCCTATTCTAGGGAGGAATGAAGT6420.13051008708803943No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA6050.12298847770757608No Hit
ATTCTAGGGAGGAATGAAGTATCCACATGTTGTTGTTCCTTCTTGATTTTC5370.10916497938672456No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT5350.10875840590669952No Hit
GGAATGAAGTATCCACATGTTGTTGTTCCTTCTTGATTTTCTTGTGTTTTG5030.10225323022629879No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG1250.043.19814340
CGTATGC1200.043.12314641
CTCGTAT1300.039.8059839
CATCTCG1250.039.59829736
ACGAGAC1250.039.59829722
CACGAGA1350.038.33168821
GTATGCC1350.038.33168842
TATGCCG1400.036.962743
CGAGACC1300.036.34459323
TCCGAGC1500.035.99845513
CTCCGAG1450.035.68812612
CCGAGCC1550.034.83721514
GCCCACG1550.033.38566618
TCTCGTA1550.033.38566638
CGAGCCC1550.033.38566615
TGCCGTC1550.033.38566645
ATGCCGT1600.032.3423644
CGTTCTT352.8187572E-432.14147610
ATCTCGT1700.030.43986937
CTGTGGA1650.029.99871429
Produced by FastQC (version 0.11.2)