FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_4_77.3410000000b1d3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_4_77.3410000000b1d3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences314360
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC9930.3158798829367604No Hit
GTACTAAGTCAGGGGATATGATTGGAGAACATGAAATGCCAACATGTATAG4020.1278788649955465No Hit
GACATAGATCATAAGATAACTCGACTTCATGTGTCCTTAGAAAGACCAGAC3880.12342537218475633No Hit
CTATAACTCTTTCCATGGGATTTTGTTTCCTATTCTAGGGAGGAATGAAGT3830.12183483903804555No Hit
ATGAAAGACAGACTATGATTCCTAGTTCTGTTCCTAAGGAAGACATCATAG3560.11324596004580736No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA3430.10911057386435934No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACCG253.88513E-544.9984111
TGCCGTC1050.040.7128545
CGAGACA1150.037.17260423
CACGAGA1200.035.6237421
TATGCCG1200.035.6237443
GTCGAAA458.6756154E-734.9987630
GTATGCC1250.034.1987942
GCCCACG1300.032.88345318
CCCACGA1300.032.88345319
ATGCCGT1300.032.88345344
ACGAGAC1300.032.88345322
CCACGAG1300.032.88345320
CAGAAGT352.790141E-432.1929241
GAGGAGT352.8163323E-432.1417230
CTATCGC352.8163323E-432.1417230
TACCGTA352.8163323E-432.1417213
TCTATCG352.8163323E-432.1417229
ACGTCGA501.9761155E-631.49888628
ATCTCGT1400.030.53463437
CGTATGC1400.030.53463441
Produced by FastQC (version 0.11.2)