FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_4_76.3410000000b1c6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_4_76.3410000000b1c6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences884642
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGTC36940.417570045283855No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC5850.037.69088741
TCGTATG6100.036.1461840
ACGAGAC6000.035.99864622
CATCTCG6100.035.7773436
CCCACGA6100.035.7773419
CGAGACC6000.035.62365723
CCACGAG6150.035.1206320
TGCCGTC6350.035.0774245
GCCCACG6200.034.837418
CTCGTAT6350.034.72310339
TATGCCG6350.034.72310343
CGAGCCC6350.034.36878215
CACGAGA6350.034.01446521
CCGAGCC6450.033.83593814
TCCGAGC6500.033.5756613
GTATGCC6600.033.4078342
ATGCCGT6750.032.66543644
ATCTCGT6700.032.573437
CTCCGAG6750.031.99879512
AGCCCAC6850.031.53165817
Produced by FastQC (version 0.11.2)