##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	H23V3BCXX l02n01 kb2_4_74.3410000000b1ad.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	27
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.888888888888886	0.0	0.0	0.0	0.0	0.0
2	36.48148148148148	0.0	0.0	0.0	0.0	0.0
3	34.407407407407405	0.0	0.0	0.0	0.0	0.0
4	36.888888888888886	0.0	0.0	0.0	0.0	0.0
5	36.666666666666664	0.0	0.0	0.0	0.0	0.0
6	37.666666666666664	0.0	0.0	0.0	0.0	0.0
7	36.81481481481482	0.0	0.0	0.0	0.0	0.0
8	37.666666666666664	0.0	0.0	0.0	0.0	0.0
9	38.51851851851852	0.0	0.0	0.0	0.0	0.0
10	38.51851851851852	0.0	0.0	0.0	0.0	0.0
11	38.592592592592595	0.0	0.0	0.0	0.0	0.0
12	38.333333333333336	0.0	0.0	0.0	0.0	0.0
13	38.666666666666664	0.0	0.0	0.0	0.0	0.0
14	38.81481481481482	0.0	0.0	0.0	0.0	0.0
15	38.370370370370374	0.0	0.0	0.0	0.0	0.0
16	38.96296296296296	0.0	0.0	0.0	0.0	0.0
17	37.81481481481482	0.0	0.0	0.0	0.0	0.0
18	37.77777777777778	0.0	0.0	0.0	0.0	0.0
19	37.25925925925926	0.0	0.0	0.0	0.0	0.0
20	36.888888888888886	0.0	0.0	0.0	0.0	0.0
21	38.592592592592595	0.0	0.0	0.0	0.0	0.0
22	38.18518518518518	0.0	0.0	0.0	0.0	0.0
23	37.18518518518518	0.0	0.0	0.0	0.0	0.0
24	36.77777777777778	0.0	0.0	0.0	0.0	0.0
25	36.74074074074074	0.0	0.0	0.0	0.0	0.0
26	37.22222222222222	0.0	0.0	0.0	0.0	0.0
27	37.48148148148148	0.0	0.0	0.0	0.0	0.0
28	36.370370370370374	0.0	0.0	0.0	0.0	0.0
29	37.74074074074074	0.0	0.0	0.0	0.0	0.0
30	36.7037037037037	0.0	0.0	0.0	0.0	0.0
31	37.592592592592595	0.0	0.0	0.0	0.0	0.0
32	37.888888888888886	0.0	0.0	0.0	0.0	0.0
33	37.2962962962963	0.0	0.0	0.0	0.0	0.0
34	37.55555555555556	0.0	0.0	0.0	0.0	0.0
35	37.0	0.0	0.0	0.0	0.0	0.0
36	38.74074074074074	0.0	0.0	0.0	0.0	0.0
37	38.666666666666664	0.0	0.0	0.0	0.0	0.0
38	38.666666666666664	0.0	0.0	0.0	0.0	0.0
39	38.2962962962963	0.0	0.0	0.0	0.0	0.0
40	38.370370370370374	0.0	0.0	0.0	0.0	0.0
41	38.74074074074074	0.0	0.0	0.0	0.0	0.0
42	38.370370370370374	0.0	0.0	0.0	0.0	0.0
43	39.25925925925926	0.0	0.0	0.0	0.0	0.0
44	38.0	0.0	0.0	0.0	0.0	0.0
45	38.888888888888886	0.0	0.0	0.0	0.0	0.0
46	37.74074074074074	0.0	0.0	0.0	0.0	0.0
47	37.51851851851852	0.0	0.0	0.0	0.0	0.0
48	37.03703703703704	0.0	0.0	0.0	0.0	0.0
49	37.592592592592595	0.0	0.0	0.0	0.0	0.0
50	37.370370370370374	0.0	0.0	0.0	0.0	0.0
51	37.55555555555556	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
2115	1	3.0
2115	2	3.0
2115	3	0.0
2115	4	3.0
2115	5	3.0
2115	6	1.0
2115	7	0.0
2115	8	1.0
2115	9	1.0
2115	10	1.0
2115	11	1.0
2115	12	1.0
2115	13	1.0
2115	14	0.0
2115	15	1.0
2115	16	1.0
2115	17	1.0
2115	18	1.0
2115	19	0.0
2115	20	0.0
2115	21	1.0
2115	22	1.0
2115	23	1.0
2115	24	1.0
2115	25	4.0
2115	26	1.0
2115	27	4.0
2115	28	1.0
2115	29	1.0
2115	30	1.0
2115	31	1.0
2115	32	1.0
2115	33	1.0
2115	34	1.0
2115	35	0.0
2115	36	1.0
2115	37	0.0
2115	38	1.0
2115	39	0.0
2115	40	1.0
2115	41	1.0
2115	42	0.0
2115	43	0.0
2115	44	4.0
2115	45	1.0
2115	46	0.0
2115	47	0.0
2115	48	0.0
2115	49	0.0
2115	50	1.0
2115	51	1.0
1210	1	-3.0
1210	2	-3.0
1210	3	0.0
1210	4	-3.0
1210	5	-3.0
1210	6	-1.0
1210	7	0.0
1210	8	-1.0
1210	9	-1.0
1210	10	-1.0
1210	11	-1.0
1210	12	-1.0
1210	13	-1.0
1210	14	0.0
1210	15	-1.0
1210	16	-1.0
1210	17	-1.0
1210	18	-1.0
1210	19	0.0
1210	20	0.0
1210	21	-1.0
1210	22	-1.0
1210	23	-1.0
1210	24	-1.0
1210	25	-4.0
1210	26	-1.0
1210	27	-4.0
1210	28	-1.0
1210	29	-1.0
1210	30	-1.0
1210	31	-1.0
1210	32	-1.0
1210	33	-1.0
1210	34	-1.0
1210	35	0.0
1210	36	-1.0
1210	37	0.0
1210	38	-1.0
1210	39	0.0
1210	40	-1.0
1210	41	-1.0
1210	42	0.0
1210	43	0.0
1210	44	-4.0
1210	45	-1.0
1210	46	0.0
1210	47	0.0
1210	48	0.0
1210	49	0.0
1210	50	-1.0
1210	51	-1.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
31	1.0
32	1.0
33	0.0
34	2.0
35	1.0
36	2.0
37	4.0
38	8.0
39	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.22222222222222	18.51851851851852	14.814814814814813	44.44444444444444
2	7.4074074074074066	29.629629629629626	48.148148148148145	14.814814814814813
3	0.0	40.74074074074074	48.148148148148145	11.11111111111111
4	3.7037037037037033	29.629629629629626	51.85185185185185	14.814814814814813
5	11.11111111111111	22.22222222222222	48.148148148148145	18.51851851851852
6	7.4074074074074066	70.37037037037037	18.51851851851852	3.7037037037037033
7	14.814814814814813	29.629629629629626	37.03703703703704	18.51851851851852
8	11.11111111111111	59.25925925925925	7.4074074074074066	22.22222222222222
9	25.925925925925924	18.51851851851852	25.925925925925924	29.629629629629626
10	14.814814814814813	33.33333333333333	18.51851851851852	33.33333333333333
11	40.74074074074074	14.814814814814813	33.33333333333333	11.11111111111111
12	3.7037037037037033	44.44444444444444	29.629629629629626	22.22222222222222
13	22.22222222222222	22.22222222222222	44.44444444444444	11.11111111111111
14	7.4074074074074066	11.11111111111111	40.74074074074074	40.74074074074074
15	14.814814814814813	22.22222222222222	22.22222222222222	40.74074074074074
16	29.629629629629626	37.03703703703704	22.22222222222222	11.11111111111111
17	22.22222222222222	37.03703703703704	25.925925925925924	14.814814814814813
18	37.03703703703704	25.925925925925924	25.925925925925924	11.11111111111111
19	22.22222222222222	11.11111111111111	37.03703703703704	29.629629629629626
20	18.51851851851852	37.03703703703704	18.51851851851852	25.925925925925924
21	11.11111111111111	29.629629629629626	25.925925925925924	33.33333333333333
22	11.11111111111111	40.74074074074074	22.22222222222222	25.925925925925924
23	18.51851851851852	33.33333333333333	18.51851851851852	29.629629629629626
24	29.629629629629626	37.03703703703704	11.11111111111111	22.22222222222222
25	11.11111111111111	33.33333333333333	33.33333333333333	22.22222222222222
26	18.51851851851852	44.44444444444444	22.22222222222222	14.814814814814813
27	18.51851851851852	37.03703703703704	25.925925925925924	18.51851851851852
28	18.51851851851852	18.51851851851852	29.629629629629626	33.33333333333333
29	37.03703703703704	22.22222222222222	29.629629629629626	11.11111111111111
30	18.51851851851852	37.03703703703704	37.03703703703704	7.4074074074074066
31	18.51851851851852	29.629629629629626	37.03703703703704	14.814814814814813
32	14.814814814814813	40.74074074074074	33.33333333333333	11.11111111111111
33	11.11111111111111	37.03703703703704	29.629629629629626	22.22222222222222
34	22.22222222222222	44.44444444444444	22.22222222222222	11.11111111111111
35	29.629629629629626	22.22222222222222	40.74074074074074	7.4074074074074066
36	18.51851851851852	51.85185185185185	11.11111111111111	18.51851851851852
37	22.22222222222222	40.74074074074074	25.925925925925924	11.11111111111111
38	25.925925925925924	25.925925925925924	37.03703703703704	11.11111111111111
39	14.814814814814813	25.925925925925924	33.33333333333333	25.925925925925924
40	22.22222222222222	25.925925925925924	33.33333333333333	18.51851851851852
41	29.629629629629626	29.629629629629626	18.51851851851852	22.22222222222222
42	22.22222222222222	22.22222222222222	22.22222222222222	33.33333333333333
43	14.814814814814813	22.22222222222222	40.74074074074074	22.22222222222222
44	11.11111111111111	33.33333333333333	37.03703703703704	18.51851851851852
45	7.4074074074074066	18.51851851851852	40.74074074074074	33.33333333333333
46	18.51851851851852	22.22222222222222	44.44444444444444	14.814814814814813
47	14.814814814814813	29.629629629629626	33.33333333333333	22.22222222222222
48	7.4074074074074066	18.51851851851852	33.33333333333333	40.74074074074074
49	22.22222222222222	18.51851851851852	40.74074074074074	18.51851851851852
50	11.11111111111111	25.925925925925924	33.33333333333333	29.629629629629626
51	11.11111111111111	22.22222222222222	29.629629629629626	37.03703703703704
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.5
29	1.0
30	1.0
31	1.0
32	1.5
33	2.0
34	3.5
35	5.0
36	5.0
37	5.0
38	4.5
39	4.0
40	3.0
41	2.0
42	2.0
43	2.0
44	1.5
45	1.0
46	1.0
47	1.0
48	2.0
49	3.0
50	1.5
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
62	0.0
63	0.0
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	27.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	92.5925925925926
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.0	88.88888888888889
2	0.0	0.0
3	4.0	11.11111111111111
4	0.0	0.0
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC	3	11.11111111111111	RNA PCR Primer, Index 39 (95% over 21bp)
ATTCCTGCACGTACTTCTTGAAAGTGCTGTCCTTGATGAGAGAATAGTCCG	1	3.7037037037037033	No Hit
ATATCATCCTGAGTGAGGTAACCCAGTCACAAAAGAAGTCACTTGATATGT	1	3.7037037037037033	No Hit
GATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTACCCG	1	3.7037037037037033	No Hit
ATATACTATCATATCATGTGCATGTAATGATACCTCTGTCTCTTATACACA	1	3.7037037037037033	No Hit
TCATCATCTTCTTTCAAGCTTCGACTGGGTACTCAAAAACCTGTCTCTTAT	1	3.7037037037037033	No Hit
GTAACAGAATCACCAGAAGAACAATCTCTAAACATAGTCAACTATCTGTCT	1	3.7037037037037033	No Hit
CAAGAATGAGACTGTATGTTGAACATTACATAGGGTGTTAACTCTGTCTCT	1	3.7037037037037033	No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACACTTT	1	3.7037037037037033	No Hit
AAAAGAAATATTGCTAGTCATTTTTCCTAGGGGTGATGGTGTCATACATAC	1	3.7037037037037033	No Hit
CTCTATTTCTGTATGTTATACTCACACAGTGGCATGGCCTGTCTCTTATAC	1	3.7037037037037033	No Hit
CTATCTAAACAATCCCATAATCCCCAAGGAAATAGAAGCAGTCATTATAAG	1	3.7037037037037033	No Hit
CTTATAACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGT	1	3.7037037037037033	No Hit
CTATGATATGGATCGAGATCATGACAGAGACTATGAACTGTCTCTTATACA	1	3.7037037037037033	No Hit
CTTTTTAATCTAGTCTCTGATTCGGCTTATGAAATTGTGGGACCCATTAGA	1	3.7037037037037033	No Hit
GAAATAAAGAGCCAAATTCATCTCCTTGGAAATACGTTAGAAAACAATCCA	1	3.7037037037037033	No Hit
CCTCTATCTGTGACTACATAGTAAAAATTTCTCATCCTTCCCTGTCTCTTA	1	3.7037037037037033	No Hit
TTCCAAATGAGATTCGAAAGCAGATAATTATGAGTATATTGAGGCTGTCTC	1	3.7037037037037033	No Hit
TATCAACGCAGAGTACGGGCTTCTTACCCATTTAAAGTTTGAGAATAGGTT	1	3.7037037037037033	No Hit
ACCTTAAAGACAAGGGTCTTGGTGTTAGGTCAAGGAGATTTGCTCTGTTAC	1	3.7037037037037033	No Hit
CATTAGACTCGTGTATCGCATCACATGTATACGGGTACCTGTCTCTTATAC	1	3.7037037037037033	No Hit
CATTTTCACATCTCCTGTCTAAGAGAGTGAAATCTATAAAAGTCCCTAGCA	1	3.7037037037037033	No Hit
TCTTTATAGCTAATATTTTCAGTTCACCTTTACATGTGGGTCATAATCTTT	1	3.7037037037037033	No Hit
GAATGGGAGCTATTTGAAGAAAGGAAAGTTTGAGCAAAGGCAAGGTACAAC	1	3.7037037037037033	No Hit
GGTTTATACTGATACAACATCGAAAATCACTTCATGAAAAACATTGCTGTC	1	3.7037037037037033	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	0.0	0.0	0.0
8	0.0	0.0	0.0
9	0.0	0.0	0.0
10	0.0	0.0	0.0
11	0.0	0.0	0.0
12	0.0	0.0	0.0
13	0.0	0.0	0.0
14	0.0	0.0	0.0
15	0.0	0.0	0.0
16	0.0	0.0	0.0
17	0.0	0.0	0.0
18	0.0	0.0	0.0
19	0.0	0.0	0.0
20	0.0	0.0	0.0
21	0.0	0.0	0.0
22	0.0	0.0	0.0
23	0.0	0.0	0.0
24	0.0	0.0	0.0
25	0.0	0.0	0.0
26	0.0	0.0	0.0
27	0.0	0.0	0.0
28	0.0	0.0	0.0
29	0.0	0.0	0.0
30	0.0	0.0	0.0
31	0.0	0.0	0.0
32	0.0	0.0	0.0
33	0.0	0.0	0.0
34	0.0	0.0	0.0
35	0.0	0.0	0.0
36	0.0	0.0	3.7037037037037037
37	0.0	0.0	3.7037037037037037
38	0.0	0.0	7.407407407407407
39	0.0	0.0	14.814814814814815
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE