FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_4_74.3410000000b1ad.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_4_74.3410000000b1ad.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27
Sequences flagged as poor quality0
Sequence length51
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC311.11111111111111RNA PCR Primer, Index 39 (95% over 21bp)
ATTCCTGCACGTACTTCTTGAAAGTGCTGTCCTTGATGAGAGAATAGTCCG13.7037037037037033No Hit
ATATCATCCTGAGTGAGGTAACCCAGTCACAAAAGAAGTCACTTGATATGT13.7037037037037033No Hit
GATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTACCCG13.7037037037037033No Hit
ATATACTATCATATCATGTGCATGTAATGATACCTCTGTCTCTTATACACA13.7037037037037033No Hit
TCATCATCTTCTTTCAAGCTTCGACTGGGTACTCAAAAACCTGTCTCTTAT13.7037037037037033No Hit
GTAACAGAATCACCAGAAGAACAATCTCTAAACATAGTCAACTATCTGTCT13.7037037037037033No Hit
CAAGAATGAGACTGTATGTTGAACATTACATAGGGTGTTAACTCTGTCTCT13.7037037037037033No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACACTTT13.7037037037037033No Hit
AAAAGAAATATTGCTAGTCATTTTTCCTAGGGGTGATGGTGTCATACATAC13.7037037037037033No Hit
CTCTATTTCTGTATGTTATACTCACACAGTGGCATGGCCTGTCTCTTATAC13.7037037037037033No Hit
CTATCTAAACAATCCCATAATCCCCAAGGAAATAGAAGCAGTCATTATAAG13.7037037037037033No Hit
CTTATAACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGT13.7037037037037033No Hit
CTATGATATGGATCGAGATCATGACAGAGACTATGAACTGTCTCTTATACA13.7037037037037033No Hit
CTTTTTAATCTAGTCTCTGATTCGGCTTATGAAATTGTGGGACCCATTAGA13.7037037037037033No Hit
GAAATAAAGAGCCAAATTCATCTCCTTGGAAATACGTTAGAAAACAATCCA13.7037037037037033No Hit
CCTCTATCTGTGACTACATAGTAAAAATTTCTCATCCTTCCCTGTCTCTTA13.7037037037037033No Hit
TTCCAAATGAGATTCGAAAGCAGATAATTATGAGTATATTGAGGCTGTCTC13.7037037037037033No Hit
TATCAACGCAGAGTACGGGCTTCTTACCCATTTAAAGTTTGAGAATAGGTT13.7037037037037033No Hit
ACCTTAAAGACAAGGGTCTTGGTGTTAGGTCAAGGAGATTTGCTCTGTTAC13.7037037037037033No Hit
CATTAGACTCGTGTATCGCATCACATGTATACGGGTACCTGTCTCTTATAC13.7037037037037033No Hit
CATTTTCACATCTCCTGTCTAAGAGAGTGAAATCTATAAAAGTCCCTAGCA13.7037037037037033No Hit
TCTTTATAGCTAATATTTTCAGTTCACCTTTACATGTGGGTCATAATCTTT13.7037037037037033No Hit
GAATGGGAGCTATTTGAAGAAAGGAAAGTTTGAGCAAAGGCAAGGTACAAC13.7037037037037033No Hit
GGTTTATACTGATACAACATCGAAAATCACTTCATGAAAAACATTGCTGTC13.7037037037037033No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)