FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_4_73.3410000000b183.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_4_73.3410000000b183.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141223
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1780.1260417920593671No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGATGC300.005132751529.9992941
ATTAGAC450.001221400724.9994093
AACTTGA450.001221400724.99940938
CAACTTG450.001221400724.99940937
GTATCAA5300.023.7983061
AACGCAG5650.021.5039186
ACGCAGA6300.019.285267
CTCTAAG706.9431384E-419.285262
TACGGGC1302.7219357E-819.03801215
TATCAAC6650.018.946922
CGCAGAG6300.018.9281258
GGTATCA2500.018.9196531
TCGCCGT600.006483790518.74955738
ACGGGGG600.006483790518.74955716
ATCAACG6600.018.7495573
TCAACGC6650.018.6085834
CAACGCA6600.018.4086575
AGAGTAC6950.017.48160211
GCAGAGT7000.017.0353139
CAGAGTA7300.016.64344410
Produced by FastQC (version 0.11.2)