FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_4_71.3410000000b169.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_4_71.3410000000b169.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTC21.2987012987012987No Hit
CTTTAAAACTGAGAAAAGTAATTTATTGAACCAAAAGAAACAGAGAGATTT10.6493506493506493No Hit
ATACTTGGTGTGGAGATTTGGAATAATTAATTGTTCCACTTGTGTTGGATC10.6493506493506493No Hit
AGGTTGAGGAAAGTAATGGAAGATCTGACTCTACCATTAATCTGTCTCTTA10.6493506493506493No Hit
GGTGCAGGTGCTCCGACCTCTGCAGCCATGACGGCGGTCATGGAAGCAGCA10.6493506493506493No Hit
GGCCCTTATGGGCTGGGCCACACACGTGCTACAATGGCAATTACAATGGGA10.6493506493506493No Hit
TTTGTTGTGTGTTGTTTCAGTTTCTGGTTTCTGTCTCTTATACACATCTCC10.6493506493506493No Hit
GTGCATGCGTTGTTGTAGAGGAATATTAACAGTTGGAACTGTATCTGTCTC10.6493506493506493No Hit
GTTGTGGAGTGACGTTACTAGTGACGTAATGAACCAATGCATCGGATAAAG10.6493506493506493No Hit
CTCTTTCTCTCTCTCATGGGAAGATAATCAGTTTATATACTGTCTCTTATA10.6493506493506493No Hit
CTAACACCTCAAACCACTGAATAACGTGGTTACGGAAATCGAATTTTTCTC10.6493506493506493No Hit
GCTCAAAATCTAGTGGATTTGAGAGAAAACAGTAAGTACTGTAACTGTCTC10.6493506493506493No Hit
CTGCGACTTTGTCAGCTCCAAAAGTGTATGAACTGTCTCTTATACACTCTC10.6493506493506493No Hit
GGAGTATCTGACATTCTCAGCTTTACTGGATCCTGCTTGTGCACCTGAGCG10.6493506493506493No Hit
GACTCTTTTAAACGCTATTCTTGATTGATATGATCAACTTTTGATGAGAAC10.6493506493506493No Hit
CAGGTGTTAGGGTGGGCTTTGTCACAATGTACAGCATGATCTCCACGATCT10.6493506493506493No Hit
ACTCAAGAGAGCATCGCTAACTACAAGGGTCCTACCTGTCTCTTTACACAT10.6493506493506493No Hit
AATGAATCCTGAATATGACTATCTGTTCAAGCTTCTGCTTATTGGTGATTC10.6493506493506493No Hit
TATCAACGCAGAGTACGGGGATGTTGAAAGATTAACAAGTAAGAATGCTAC10.6493506493506493No Hit
GTGTCTCTGCTACATCTCCAGCAGACTCTTCCTTCTTTTCTGTGACAACTT10.6493506493506493No Hit
CCTTTCTTAGCTTTAATTGCTTCAACCAATTTGTCGGCATCATCAATCTCT10.6493506493506493No Hit
GATGTGTAAAGTTTGTGTTATGAGGTTCTCAATTTTAGCAATGATGAGACT10.6493506493506493No Hit
CTCAGCGACGGACCGGGCCCAAGTCCACTGGAAAGTGGCGCCGGAGAGGGT10.6493506493506493No Hit
GAATACGGGGACGCTAGTTCAGGTGGAGTCATCGTTGAAATACCACTCTGG10.6493506493506493No Hit
GGGTTAGTCGGGTCCTAAGCTGAGGCCGACAGGCGTAGGCGATGGATAACA10.6493506493506493No Hit
CGCGTACACTCAGTGGAGAGCGTGTACATGTGCAACCTGGCAGCCAACGAC10.6493506493506493No Hit
GCATAAACCATGCCTAGATACCCGAAGACCGGTTTTCCCGAAAAAGTCGAA10.6493506493506493No Hit
GTCTTCTTCTTCTTCTTCTTCTTCTTCTTTCTTAGCGCACATACTGTCTCT10.6493506493506493No Hit
CTTGGAACCCATTTCTCTCTCTTTCTCTTTGGAAAATGCTTCTTCTTCTTT10.6493506493506493No Hit
TCCCTGAACCGCACGCTACGGAGGGTAAAGATGCACTGGTGGCACCAGAGC10.6493506493506493No Hit
CTACAGTACCTATTACCCAAAGAGGAATCCTTCCAGTAGTATCAGCCATTT10.6493506493506493No Hit
ATACAAACTCTATTCAGCCGAAGGGAAGATGAAGATAACTTTGAGAAAGAG10.6493506493506493No Hit
CTCTTGCAACATCTGTGGATGATACTCCAAAATCTCTCTGTACATGCTGAC10.6493506493506493No Hit
CACCACTCCTTCCGGTATGGCTTCTCCGCGGACTGAACGCTCCCCTACCAG10.6493506493506493No Hit
ATTCTAAAGTAGTTTTGGAAAGTAATTGTAGCAAGTGTTTTACTTTCAGCT10.6493506493506493No Hit
CTTTCAGATACTCGATAAAATGATCCTCTTGGTATATATCACCAAATTGGC10.6493506493506493No Hit
TCCTTAGACATCTCTCCAAAACGCTGTAACCGGAAAGTTTCTCACATAGTG10.6493506493506493No Hit
GGAACAATAATCAATTTTAGCTTCAATTGTTTGTAAAGGAACTCTGCCTTC10.6493506493506493No Hit
GTATTCGACGTAGAGAACAGAGATGAGATAAGCAGTCCAGCTTCTGTCTCT10.6493506493506493No Hit
GTTGTATCCTCTCACTCATGTATTTTTTTGTATCGGTTTCCTCCGGTTTTA10.6493506493506493No Hit
GACTAAAATCGGATCTATAACTTTTGTTCTTTGAGAGACTTATTTCTCTCT10.6493506493506493No Hit
GATCATTTGCTTTAGTGTGAATGATCCATTTGTGATGAAGGCATGGGGAAA10.6493506493506493No Hit
ATGCTGTTTCTTGCTATATCAAAAGCTTCCTTTGTCTGCTCAGCAGTAACT10.6493506493506493No Hit
GTCCGGGCACGACGGGGCTCTCACCCTCTCTGGCGCCCCTTTCCAGGGAAC10.6493506493506493No Hit
CATTTAGCCTGACGCAACACAGAATCTGCTCCGGTAGCTCCTCTGGTTTGT10.6493506493506493No Hit
GTACCGTGAGGGAAAGGTGAAAAGAACCCCGGAAGGGGAGTGAAAGAGAAC10.6493506493506493No Hit
TTCGTAGAGATTATTCTTGCTATCATCTTGCCTCCTCTCGGCGTCTTTCTC10.6493506493506493No Hit
GCTTTAAAACAACGTATGGATAGTTTAGCAGAAATGAAAAAGCTTAAACAT10.6493506493506493No Hit
GTAGTAACTGCTAGTAATCGTTCTGAAACGTTCCTGGCCTGCCGTATCCCA10.6493506493506493No Hit
ATGTAATGGACATTACTCTTAAATACATTGATCCGACATACATGATCCGAG10.6493506493506493No Hit
ACATACAATCGTCGCCATCTCCGGCGAACGCCTCCGACACCAGCTCAACAT10.6493506493506493No Hit
GGATATATCTCCACCAAGTTCTTCAAAAAGTACCTGTCTCTTATACCATCT10.6493506493506493No Hit
GACACGGAAACTGAAATCACTGATCTTAAGGAGAGAATTGTGACATTGGAG10.6493506493506493No Hit
ATAGATTAAGTAAAGCAATGGGAGGAGCAGAGAAAGTGGAGATAGAGCATG10.6493506493506493No Hit
GGTCCGGGCACGACGGGGCTCTCACCCTCTCTGGCGCCCCTTTCCAGGGAA10.6493506493506493No Hit
CTTCCGAAGAGTATAGTCATCGACTCATGCGCCCGAGCTCGGCGTGGACAC10.6493506493506493No Hit
TCACCATTTTGCTTGTAAACTGAATCACCTCCTTAAGGCTGTCTCTTATAC10.6493506493506493No Hit
ACCTTATTGACAACTTTTAAAGACAACAAAATTCGCTGTATCGAGGGAATA10.6493506493506493No Hit
GGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGGAGCTGTC10.6493506493506493No Hit
GTCATTGCAGTGAATGGCATTTCTCTTTCTAGATCAGTTGTCTAAATATGT10.6493506493506493No Hit
GGCTAACTTCCTTCGAATGTTTTAGTTGTTTCCTTGTATTTTACTCAATGT10.6493506493506493No Hit
CTCTTCTTCTGTCACATCTCTATCTTTCTAAATCGTTTTCAATCTTTTAGC10.6493506493506493No Hit
GATCTAAGTTACTGTGATGGTGATTTCGTACAGAGATTGTTTTTGCGGCTA10.6493506493506493No Hit
ATATTAGATGCTGCAACGCATTTAATGTGGTAATCATATGATATTAGAGGA10.6493506493506493No Hit
TCTCTGTTCACTCTATCGCCGCCGGTAAAAAAGTCATTCTCTTTGGTGTTC10.6493506493506493No Hit
ATTCATAATACTGCACAGTAGTATGCTTAGGCTTTACTATGTATTAGCCGT10.6493506493506493No Hit
GTTCAGAGGAGTGTTGAATGTGTGTTCTTTCTTTAGCTATGACCTGTCTCT10.6493506493506493No Hit
GTAGAGAAAGAAACCAACTTCCTTCATCACAGTGAGTTTCTTGTTTTGTTT10.6493506493506493No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCAT10.6493506493506493No Hit
GGAGATGGCAGTTTATGATCAGAGTGGGGATAGAAACAGAACACAAATTGA10.6493506493506493No Hit
GTATCAACGCAGAGTACGGGAAGTTAAGAGAAAAGAAAAAAAAAACCGTTC10.6493506493506493No Hit
CATGGGCTCATCACAGTTGGATGCGAGCACACACAGATGGGCTTGGCGCTT10.6493506493506493No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGTTGGAAAATTTAG10.6493506493506493No Hit
GAGGAGAGAAGAGGAGACCTTGAGACCCATCTTTGGGTGTCTTTGTTTATT10.6493506493506493No Hit
TCGTAATCCATTGTTGTGTGTTTGTTTTCTAGTTTGAAGAAGAATTTGCCC10.6493506493506493No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACATTT10.6493506493506493No Hit
CTCCTATCTCGGAGTTGATAATCACGGTGGTAGAGAATTTCTTCCATGGCC10.6493506493506493No Hit
ACCTTCGTGACTGATTTATTTCAAGGGGCTAAGTATTTCCGAGTTAAGATG10.6493506493506493No Hit
CTTTGGATCTAATGCAAGAACAGAACACCATAAGAGTATCTTTTCACTTCT10.6493506493506493No Hit
CTTATACACATCTCCGGCCCACGAGACAGGATGTGATCTCGTATGCCGTCT10.6493506493506493No Hit
ATTTTGAAGGATTTGGGATTAAACGGAGGGAGACATTAAAAACAAGAATTA10.6493506493506493No Hit
GAATATGACTATCTGTTCAAGCTTCTGCTTATTGGTGATTCTGGTGTTGGA10.6493506493506493No Hit
GTACAATAACTGTGAATCTTGGGACATAATCAAAGTCTGTCTCTTATACAC10.6493506493506493No Hit
CCAATAGGGAACGTGAGCTGGGGTTAGACCGTCGTGAGACAGGTTAGTTTC10.6493506493506493No Hit
CTACATGATGAATACCTATCTCGATTCGCTTACAGACTGTCTCTTATACAC10.6493506493506493No Hit
CTACAAGGGTATTGGTGACTGTTTCGGCAGGACGATTAAGGATGAAGGTTT10.6493506493506493No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT10.6493506493506493No Hit
GAGTAAGGCGAAGGATTTGGAGAAAGTTGAGAATCCATTTAACAAGAGGAG10.6493506493506493No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA10.6493506493506493No Hit
GTTCTGCAACAGCACAATCCGAAGATTTGTTAAGTTGGTAATGCTTGGAGA10.6493506493506493No Hit
CATAGAAGGAGAAAGCACTGAAGCATTGGGTAAATTGATTGAAGGAGAAGA10.6493506493506493No Hit
TTTTCATTTCGTCTGGCTTGTAGTTAACTTCGATTTTGTCTGCATCCCACT10.6493506493506493No Hit
CTTTATGATCTACTTCCTCTCCTATCTATTTTTCTTTATTATCCCCTTTCA10.6493506493506493No Hit
ATCATGGACAGCCATATACATTGCATTAGACAACGTTGGAATGTGGAACTT10.6493506493506493No Hit
ATGCAGCGCTTGTAACAATTTTTTATAATCTGCTATACAAGCTGTGGTAAG10.6493506493506493No Hit
GTATAGTTATGGTCTTGACCTTGTCCCAGTTCCTGTCTCTTAAACATCTCC10.6493506493506493No Hit
GAAGTATAAAGATGAGAAGTACGAGAAAGCTGAGAAGGGTTCAAGGACTGT10.6493506493506493No Hit
ACCTATCATACTCCATAAGAAACCCATCAGCTTGCTTTCTCAAAGCCTCTG10.6493506493506493No Hit
TCACATTATAGGTTAATGATCTCACAGTCCACGAACGACGTAGTCGTAGTG10.6493506493506493No Hit
TTCTTGTTCTGAACGTTGATCATCTGTTCGTCAACTTCTTTCGCTGTCTCT10.6493506493506493No Hit
GATTCTCACTTGCCATTCGTTACTCATGCCTGCATTCTCCTGTCTCTTATA10.6493506493506493No Hit
GTGGGAGCAAGTGTGTGTGGCTGCCGTCGCATGCTGTTGATGTTGTCGTTC10.6493506493506493No Hit
GAACAGATTCAAGAGAACAAATTCAGATAGAGAATTTACATCAGACCACGA10.6493506493506493No Hit
GAATATGAAGAGTATACAACCTTGAAGACAATAAGCTCTATTCAGCAGAAC10.6493506493506493No Hit
TCGTCAGCTCGTGTTGTGAAATGTTGGGTTAGTCCCGCAACGAGCGCAACC10.6493506493506493No Hit
GAGTACGGGTATTGTTATTTATTGTCACTACCTCCCCGTGTCAGGATTGGG10.6493506493506493No Hit
ATCAACGCAGAGTACGGGGATACGCCTTGAGCGCACTAACAAGAGGCATGC10.6493506493506493No Hit
ATCAACGCAGAGTACGGGACAACTCTCTCATAATCTCTCTCCCTCACTCTA10.6493506493506493No Hit
CAGTGAAAGTGATCGATACTCAATTCTTATCCTCATTCTGGAAGTGTGTGT10.6493506493506493No Hit
TTCGAGAAGACATATTTTTCAAAAGCTAACTTGGAAGCCTGTCTCTTATAC10.6493506493506493No Hit
ATATTATCGTCAACTTCTGATAATTCTTCTAGCTGTCTCTTATACACATCC10.6493506493506493No Hit
GAAAAGCTCCCTCGCCGTAAGTCCAAGGTTTCCTGCGCAACGTTCATCGGC10.6493506493506493No Hit
CCTTTCAGAGTGACCAAGGATAACCCATGGAATATCCAAGTTCACAAGCCT10.6493506493506493No Hit
ATCCATTATCTTTTGGAAAATGTTGTTGCAGACAGTGTAAGAACTGTCTCT10.6493506493506493No Hit
ATTTGTAACCATGTGAAATTACAATGTTCTGGCTCTTAGAACGATGTTAGA10.6493506493506493No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTCCCTCC10.6493506493506493No Hit
CATCATTAGTAGGTACGTCTTGGTGTCCATTAGTGGCTGTCTCTTATACAC10.6493506493506493No Hit
GAAACTATATTGTCCCCTTGCCCATTTCAAGACAAAATCATAAACCTGTAT10.6493506493506493No Hit
ATTGAAATCTTCATTCCCAAAAGTATCACATGTTGCAATCTGTCTCTTATA10.6493506493506493No Hit
ATCTTCTCACACTCTTCGGCCACATTTCAATTCTTGCTCTCGCAGTATTGT10.6493506493506493No Hit
TGAAAACTTAGCGAACTGAAACATCTAAGTAGCTAAAGGAAGGGAAATCAA10.6493506493506493No Hit
CCAGAAAATCAAAAGAACGAAAAACAAATTATGTCTGTTTAAAAGCATATC10.6493506493506493No Hit
CTTAAAGGCTATGACCAATTGCTTAATCTTGTTCTTGATGAAGCAGTCGAG10.6493506493506493No Hit
ACAACAATATAATGATACTACCTCGAATAGTTACAAGATGTGGTTGAACAA10.6493506493506493No Hit
CCTCTCATCTCCAAGTATCTCTTCAACCGAACCTCTGGGAATATTGTTGTC10.6493506493506493No Hit
GAGTACGGGCAGCTTTCCCTTCCAGTTTTTTCGACCGGTTTGAAGCTATAA10.6493506493506493No Hit
GATTTTTTGGCTGCTTTTACTGCTTAGCCTCTCATCATTGACATCAATATC10.6493506493506493No Hit
CTTCAATATCATCTTGAAACTCTGAATGTGACGCAGGATGTTCTTGTTGCT10.6493506493506493No Hit
ACTCTCTCCAGCTTTTAAAAGTGTACCAAGTACTGTAGAACCTGTCTCTTA10.6493506493506493No Hit
GCATTATTCATATGGGTTTTGCGCAAAACCGTCTCCAAGAATTGAACCAAG10.6493506493506493No Hit
ACTTAGATGTTTCAGTTCGCTAAGTTTTCAAAGTCCAAAGAGCGCAGACTA10.6493506493506493No Hit
CTCTAAATCTTCCTTTCTCAAAACATAGAACTGACTTGTGTATCCTTTGCT10.6493506493506493No Hit
GAGGAAATTGTTTCCTCGAGTCGTTGATTTCACACTGCCGAGAAAAGCCTG10.6493506493506493No Hit
ACCTTAGATTGTTTTTCACCTCAATAGAAATAATCATACACACTTGCGCCT10.6493506493506493No Hit
CGACGATAGATTATGATGTAAATTCAAAGAGATGGAGTGCAGACCTTGGTT10.6493506493506493No Hit
ATCCATGGCCATGTTAGGATCAGAAAACATAGCAGCTTGTGGATTTTGAGC10.6493506493506493No Hit
GCCGAGAAAAGCATCGGCGTGAGGTTTTAGCCGCCCGTACTCTGCGTTGAT10.6493506493506493No Hit
ACATACAGCAGTTTAGATTCACAATACAACAAGCGAAACTGTCTCTTATAC10.6493506493506493No Hit
GAGAAATCCATGGCGATTCCGATATTCCTGTCTTTAGGCTGTCTCTTATAC10.6493506493506493No Hit
CATTTAAAGAGTGCGTAATAGCTCACTAGTCGAGTGACCTGTCTCTTATAC10.6493506493506493No Hit
CTCCACCAGCCAACATGGAAAGGTTAGAGGGAACGGACTGTCTCTTATACA10.6493506493506493No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCT10.6493506493506493No Hit
AATACGAACCGTGAAAGCGTGGCCTATCGATCCTTTAGACCTTCGGAACTG10.6493506493506493No Hit
GCAATATACTCAAATTTGCAAAACAAAGAGAAAGATTAAGCAACGGCCGCC10.6493506493506493No Hit
GTCATAAACACAACCACAATCGTAGTCAAAACAAGACCAAGCAAAGTCTCA10.6493506493506493No Hit
TCTTATACACTCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGTC10.6493506493506493No Hit
CTTTCGAAGTTCGCGCTTAAGCGCTATCAGACGGGTTTCCCCCGACTCTTA10.6493506493506493No Hit
CTTTAACATTTGGTACGTCTACTGGACTTGGCATAAATTCAATTATTGCGT10.6493506493506493No Hit
GATATATTTAGAAGAGCTTTTGCTTCTCGTGTATTTCCTCCTCATGTCCTG10.6493506493506493No Hit
TCTGTATCCGGTTTGCTTCAGTTGAGAATTGCAAAGCATTTATGCAAAAGT10.6493506493506493No Hit
CCTACAGGACCGTCTTTAGCAAGTCCAAAATCAGATAATTTGGCGTTGTAA10.6493506493506493No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTATCGACCTTC10.6493506493506493No Hit
CCTTAAGTGAAAAGCTTCTATTGGGGAATGTGTTTACTTGCTGTCTCTTAT10.6493506493506493No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers