FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_4_70.3410000000b15d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_4_70.3410000000b15d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1032656
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTC13650.13218341829224833No Hit
TTATTATGCCAAGCAAGTCAAACACATGCTTGTTGATTGAAATCAAGCTAT10420.10090485117987015No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACG1900.035.52543618
CCGAGCC1950.034.6145314
TATGCCG2150.031.39457143
GAGCCCA2150.031.39457116
GTATGCC2100.031.0706642
TCCGAGC2250.029.99925613
CGAGCCC2350.029.68011715
ATGCCGT2350.029.68011744
TGCCGTC2200.029.65835845
AGCCCAC2400.028.12430417
ACTCTGG2500.026.09935427
TAAGCGG709.3080234E-725.7136518
CCCACGA2650.025.47106719
CGAGACT2650.025.47106723
GACCGAC551.368301E-424.5448477
GCCGTAG651.5238482E-524.23017125
TCTGGAC2800.023.30299429
CCACGAG3100.023.22523120
GTCCTAG1003.601599E-822.5245271
CGTATGC3000.022.49944541
Produced by FastQC (version 0.11.2)