FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_4_69.3410000000b140.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_4_69.3410000000b140.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1391921
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTC41200.2959938099935269No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG5750.034.4332743
GCCCACG5700.034.34059518
CCCACGA5800.034.13643319
TGCCGTC5700.033.94587745
ATGCCGT5750.033.65069644
GTATGCC6150.032.92538542
CCGAGCC5950.032.89771714
CGCTATG5900.032.7951730
CGTATGC6150.032.55954741
ACTCGCT5950.032.5195827
CTCGCTA6150.031.09619728
GACTCGC6150.031.09619726
AGCCCAC6350.030.8254217
CCACGAG6550.030.2276820
CGAGCCC6500.030.11406315
CTCCGAG6600.029.31689512
CTCGTAT6750.028.99872839
TCGCTAT6600.028.97629
CGAGACT7050.028.4030123
ACGAGAC7250.028.24013922
Produced by FastQC (version 0.11.2)