FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_4_68.3410000000b133.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_4_68.3410000000b133.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC21.6528925619834711No Hit
TCTTTACACCTATCTCCCTCCTTGACGCCGTCGCCGTCGTTACCGTTGGTG10.8264462809917356No Hit
GGATTAAGAACGTTGAGGGAAGTTTTGTTCCCATATGGTGATCTGTCTCTT10.8264462809917356No Hit
TATTGGAATGGAATGGAACGGAATGGAATCAACACGAGTGGAAAGGAATGG10.8264462809917356No Hit
GTCCATTTTAGAACTCGTCTGCATTCAACTATCTGTAACCATGCTTCCTGT10.8264462809917356No Hit
CAACAATATTTACGCAGTTGGTATTAACTGCGCACCTCCTCAATTCCTGTC10.8264462809917356No Hit
AGCTTAGCCATGTGTTTTGTTGTGGTTTTGGTTACTATGGTTGGTTTGTTT10.8264462809917356No Hit
CTTATACACTCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTCT10.8264462809917356RNA PCR Primer, Index 20 (95% over 22bp)
TTGATTACTTATCCTGATTTAGCCCTAAACCATATGTCATGCTTCCAAGGG10.8264462809917356No Hit
CTTCGGATTCGATCTCTTTGGTTAAGATTGAAATAGTATTTTGGAGGTGTT10.8264462809917356No Hit
GAGTACGGGGACTCGGGGGCATCCGAATGGGGAAACCTACTAGACACACTC10.8264462809917356No Hit
AGCCAAGTGGTTGTGGAAGCAAACCTTAGGGAGAAAGAGAGCTTTAATGCT10.8264462809917356No Hit
ATCACTGTCATCCTTCTTCTTCTTTGATTTCGATACAGACGGAGCCGGAGC10.8264462809917356No Hit
ATCAACGCAGAGTACGGGAAGGGTATCAACGCTGTCTCTTATACACATCTC10.8264462809917356No Hit
GTTGTGGACGACGTTGTCGCCGCAGATGAGGCTCGTCGTGAGGCTATTGCT10.8264462809917356No Hit
CCCTAATAGTCACTTAACACTTCAAGAGTGGAAAAAGCAGCAGTAACTGTC10.8264462809917356No Hit
TCTTAATCCATTTGTAACTCTTTCTCAAAGTTATCTTCATCTTCCCTTCGG10.8264462809917356No Hit
GTATTGAATAGGATTGAAGAGATTGATGACTCTTGGGATGATCTTGAGAGA10.8264462809917356No Hit
GTGTAGAGAAAGGCCATTGACTTGTTTGAGTTAATTTTATATCCAGCTACT10.8264462809917356No Hit
TCTCAGTAACCCGAGGAAAAGACATCAATAGAGATTCCCCTAGTAGCTGTC10.8264462809917356No Hit
AGGTACAATAATGTGAGTAGAAATTGTTAGTGTCTTTGATCCACTTCGTAT10.8264462809917356No Hit
CCCAATACGAGTTATGTCGTATTATCGATACGTAAATACATAGCGTATCGA10.8264462809917356No Hit
ATGTTATTCTCGAGGGCTATTTCGTTTTCTCCCCTGAGTTTTTCAAGGACA10.8264462809917356No Hit
GTTGTTTAATACGAAAATACGCGTAAAACCTTACCTCCTGTCTCTTATACA10.8264462809917356No Hit
GTTTTTATCGGAGATCGATTGCGCCTCGATTCTCTAACAACATATATCCCT10.8264462809917356No Hit
TTATAAATCCATAGCTTCGGTATTCTGTTTATGCCCGATTATTATCCTGTC10.8264462809917356No Hit
CAATAGAATGGAATGGAATGGAATTAACCCGAATAGAATGGAATGGCTGTC10.8264462809917356No Hit
CACTACGTCCATGAGAAAAAGGAATGGATGTTTCAGAGAGTTCTGGATCTG10.8264462809917356No Hit
GGTAAATTCCATCTAAAGCTAAATATTGGCGAGAGACCGATAGCGAACAAG10.8264462809917356No Hit
TTGTGATACCGACCAAGTATCTTCAATATCCTGAATTTAGGCTGTCTCTTA10.8264462809917356No Hit
TGCTACATCCATGGTAGCTTATGCTTCCAAAGGTACACATCAATTTCTTTA10.8264462809917356No Hit
TCACAAAGTCGCAATGGACAGAACAAAAAACCAAACTGTTTTATCGATTTT10.8264462809917356No Hit
CAATGAATGTTCTCTCTCTCGCTTGTTATGCTTCTACTTGGTTTTGTGTGT10.8264462809917356No Hit
CGTATATGTACGTTTGCGTTACTATGCCGCTGGCTGAGTTCTGGAGTTAGA10.8264462809917356No Hit
GTTTTAAGACAACACTATAAGGACCCAAAAAGGAGACCAAAGACAAAGACA10.8264462809917356No Hit
AATTGTACCCTTTCACAGACTCTGTGTGGTCTCTAGTATTATGTCTGTCTC10.8264462809917356No Hit
ATATTATGTTCGTGATCAAGAACAGAAAACTGATGATCTTATAGGACATAA10.8264462809917356No Hit
GGTCACAGATGTCTGCTGATGTATGCATTCAGCTTTTACACCTAAAGCTGT10.8264462809917356No Hit
GCTCCATATCTAAGGAAGATAGATCTAACGATGTATAAACACTGTCTCTTA10.8264462809917356No Hit
ATTGTTGTTTGTTAAAGCGTGCAACTTTTTCATTAGACATGTTCAATATCC10.8264462809917356No Hit
CACCATGGCAATATGTATTTCCCTTGAAACATTGCAAACAAATATACCTGT10.8264462809917356No Hit
GATTATCACCCTCTACGATTGACCTTTCCAAGTCATTCCTCTAAATCTGTC10.8264462809917356No Hit
TAACAAAGCTGAGGAAATTGATTCGTTTTCCTCTTTTTACATCTGTCTCTT10.8264462809917356No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCTTTTCAACCCCCCCC10.8264462809917356No Hit
GAAATACAAAAAGCTAAGAAGCAAGTATCATCGACCTGTCTCTTATACACA10.8264462809917356No Hit
GTATTGATGAGATCATTTGCTTTAGTGTGAATGATCCATTTGTGATGAAGG10.8264462809917356No Hit
AGTTGATCCAGATTGATAACGTTTGAGATTGTTAACGTTAAGTTAGCAGTG10.8264462809917356No Hit
ACAGTATCAGTTATAAACTCTCTCGACTCCTATTCTCTTAGCTTCAAGCTA10.8264462809917356No Hit
CTCTCACCTACTCCGGCAGGCCATTCCAAACCACTTCACCTAACAACAATG10.8264462809917356No Hit
CATGTTTTACTTACCACAAACACAGTATAGGAGAATTCCTATCATCCAACT10.8264462809917356No Hit
TTCTTATATAGGAGCATCACTGGCAATGCCTTGTCGAAATAACTCCTTATG10.8264462809917356No Hit
ATTACTAGGTGTGCTAGAGACACTAACGACATCAAGACTTGAAAACATTGG10.8264462809917356No Hit
ATGATGATGTTGATGGCATTGATAATGATGACTGTCTCTTATACACATCTC10.8264462809917356No Hit
CGTGAAAGGGAAGGAGATTATTGATGATGCTCCTATTGACCTGTCTCTTAT10.8264462809917356No Hit
GAATGAGCCATCTCCTCCCATAAGCATCTGGAGAACAGTGGCGGTGACTGC10.8264462809917356No Hit
TAAACATTGAAGAGACAATGCGGTTAACAAATCGGGTTTTCTGGTAGTCGT10.8264462809917356No Hit
GAATATGAGGATGTCAAAAGTGTGTATATAGATCTGTCTCTACACATCTCC10.8264462809917356No Hit
CACTCGTACAGCGAACAGCCGGGATTCGGAAGGAAGTGTCCGAGATTCGTG10.8264462809917356No Hit
CTTCTGCGTCAGTACTTCTCTCAATCACAATCTTGTAGTACAGGAATTTCT10.8264462809917356No Hit
CTCCAGATCAATCCATGGCCATTATGGACCTCCTTGAGCACCGGTGGGCAG10.8264462809917356No Hit
GGAAAGGTTCCATGTGAACGGCACTTGCACATGGGCTGTCTCTTATACACA10.8264462809917356No Hit
GATCTAATTCCAGTTCTGCACAAACTATTCCACAAAATAGAAGTAGAAGGT10.8264462809917356No Hit
GATGAAGAACATACATTTAGACCCTGAAGCAAAGCCAAAGCTTTGATATTC10.8264462809917356No Hit
GTAAAGATCCCGATCTTCCTAGTGATATGCGGGACAAACATTGTTATAAGT10.8264462809917356No Hit
GTTCTAGCGATAGTACTGGTTGCAGAGAAAAACATTGTGTACCTGTCTCTT10.8264462809917356No Hit
AGGCCATGGGGAGATACCGTCTGTGATCCCTGTCTCTTAACACATCTCGAG10.8264462809917356No Hit
GTAGACAGAGAGATCCTATATCATCATGTTCTGCCTGTCTCTTATACACAT10.8264462809917356No Hit
GAATACACATGTCGATAACATGACATAACTCATGCTGGGTTCCCCCATTCG10.8264462809917356No Hit
CATTGTACCAGTATCTCATGCACTTCCTAGGACCTGTCTCTTATACACATC10.8264462809917356No Hit
ACATTGGAAGATGAACCACCGTTGGATCCTTGGTTTTTGTTGAACATATTG10.8264462809917356No Hit
GTTCTATAGTAGGAGAGCGTTCTAATCAGCATAGAAGCTGTCTCTTATACA10.8264462809917356No Hit
AGACAAAAGTAATGATATGGGTAAAAGTGACAAGCATATCTACAGATGCTG10.8264462809917356No Hit
GGCTAACATTGTCCATTGTGATATCTTCGAAAAGAATGTTGGAGGCTGTCT10.8264462809917356No Hit
GCTCTTAACATGTCTCCAGTGGATAAGTGACACAAGCAATACCTGTCTCTT10.8264462809917356No Hit
ACTCAGATGTGTGTAACCATAATGTTCAACAATATAAGCACACTGGGTACC10.8264462809917356No Hit
GAGTACGGGGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCCT10.8264462809917356No Hit
GAAGAAAAATTCAAGCCACAATCATATAACACCTGTCTCTTATACACATCT10.8264462809917356No Hit
ATGGTAACCGTTGCAAGATACATGAATGTTACACCTGTCTCTTATACACAT10.8264462809917356No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCCTGTCTCTTATACACATCT10.8264462809917356No Hit
TATGGATACATTGTTCAGATTAAGCTGAGCTCCTCTTGCTCTACCTTAATG10.8264462809917356No Hit
AAACATATTACCGAAGCTGTGGATTGTCCTTTGGACAATGGTAGGAGAGCG10.8264462809917356No Hit
ATATATGATTGACCATAACACGATGTGATTACAATCAATTACCTGTCTCTT10.8264462809917356No Hit
GGGTAATGCTGGTAAAGTGGTTCTCACAAAAGATACTACAACGATAGTTGG10.8264462809917356No Hit
GTCTCTCGGCGTTCTTCAATCGAAAGGTAAAGAGTTTGAAGAAGTAAGTCA10.8264462809917356No Hit
CATTTGTAATAGCCATTGTAATCACCATTTTCATTCCTCTTGATGGTGAGC10.8264462809917356No Hit
CTCCTTTCTTTCCCGAATAACCTCTTTTAGATAGGGTTCAAGGTAGAATTT10.8264462809917356No Hit
GGCCCTCGAACAATGAAATTCCTTGATAATTTCCATGTGCAAACAAAGAGA10.8264462809917356No Hit
CTCTAAGAAACATTATCATTCGCTTGTTGTATTGTGAATCTAAACCGCTGT10.8264462809917356No Hit
GAAGATAACAAAGGAGAGGCATGTAGTGATGATGTCCAAGATCTGTCTTTA10.8264462809917356No Hit
GTCTTGTGTTACATGAAAGAATCCATTCACATTTTCGAAAGTCGAAACAGA10.8264462809917356No Hit
GTTCTGATGGGTATTGGGAAGAAGTGGAAGGAGATACTTGATGCTGTCTCT10.8264462809917356No Hit
ATCTTGTTGTGAGAGATGCTTTTTTCAGCCGTATGGTATATAATCTGTCTC10.8264462809917356No Hit
GTTGTTACAAGACTTCCGTTGATAAATCAAAAGAGAAAAAACGAAGACACT10.8264462809917356No Hit
CTCACGCTTACTAGGAATTCCTCGTTGAAGAGCAATAATTGCAATGCTCTA10.8264462809917356No Hit
GACTTTGAAAACTTAGCGAACTGAAACATCTAAGTCTGTCTCTTATACACA10.8264462809917356No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA10.8264462809917356No Hit
AGAAAGTATTTAGCAAAGTTATTCAGAGCCACATCATCTCTGTAACATATA10.8264462809917356No Hit
GTGCTGACAATGGTGGCTGCATGCGCTCTGACAAAGGCCCATGGAAAAATC10.8264462809917356No Hit
GATTTCATCGTTTATGTGTCTTGAGTTTTCACCAAGTGTCTCGGCTGTCTC10.8264462809917356No Hit
ACTTTTACTTCCGTCTTCGTTTGGTGATCTTAGGAACTGTGATTAGAAGCA10.8264462809917356No Hit
AAATAGTGCTATATACTCACCAGGTGATGCTACTAAAAAACAAACTTATAG10.8264462809917356No Hit
GTGATGAGATCACTTGGGCAGAACCCGACAGAAGCCGAGCTCCAGGACATG10.8264462809917356No Hit
CAGGAAGAGTTACTTGCTTACATTGATCGTCAAGAAGAAGCTCAGTTCCAG10.8264462809917356No Hit
CGCCACTACTGACAGAATCATTATTATTTTCTCTTCCTGCGGGTACTGCTG10.8264462809917356No Hit
ATCTTCATTCATCCAATCCGTAACGTAATTGCTGTCTCTTATACACATCTC10.8264462809917356No Hit
ATAAATAATGTGGAGAAACCGAAAAAAGAAGGAGATAAAAGCTGTCTCTTA10.8264462809917356No Hit
GAATACAAGAGGACAAAGGAAGAAGAAGCCCTGAGTCAGAAGAAAGAAGAA10.8264462809917356No Hit
CTTTAAACCATGTCCTGGAAGACTCTTTTCCAAGCGCACAAAACCACTTGG10.8264462809917356No Hit
ATTTAGAGGAATGACTTGGAAAGGTCAATCATAGAGGGCTGTCTCTTATAC10.8264462809917356No Hit
CTGTAGTATGCCTGTAAGTTAAATCTACAGGCGAATAGTCCATCCTCAAAC10.8264462809917356No Hit
GATCAAGATGAGAAGAAAAACGATTCCCACTCGTATGCCGTCTTCTGCTTG10.8264462809917356Illumina PCR Primer Index 5 (95% over 23bp)
GTCGTTCAGTAAGTTTAAATTGTTCTTGCAAGCTTACCATTGCAACTTTAT10.8264462809917356No Hit
CCATTATTCTCAGATAGAGAAAAGAAGAAAGAATGGTGGGAAGCTGTCTCT10.8264462809917356No Hit
GACTTAATACATCACCCATACCTAGTATACGAGATGCCATACGCTCTGGAT10.8264462809917356No Hit
CTTCCTGGAGATCAGTGATTCTGATTTTGATGATTTTGCCATGTTGTTCTC10.8264462809917356No Hit
GCTTTATTTGTAACTTCCTCCTCAACTGTAAAGCTGTCTCTTATACACATC10.8264462809917356No Hit
GATAAAGAGGGCCACAAAGATGTTCTTCATCTCCATTATTTCTGTCTCTTT10.8264462809917356No Hit
CAATGATTTTTGGTTCGTAATTTCAACAAGAATCAGTCTAAGACCTGTCTC10.8264462809917356No Hit
CTACTGTTGGTGGAAGAGATGTATCAACAATCGTATGACCGATAGACAGCA10.8264462809917356No Hit
CCCTTTGTGGGTTCTAGGTTAGCGCGCAGTTGGGCACCGTAACCCGGCTTC10.8264462809917356No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers