FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_4_67.3410000000b126.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_4_67.3410000000b126.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1721879
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACCGC1650.035.45317528
CGCGCTT352.821598E-432.14161312
GCCCACG1850.031.620418
CGAGCCC1850.031.620415
ACTACCG2100.027.85606427
TGCCGTC2050.027.43796245
TATGCCG2100.026.78467843
CCCACGA2400.025.3115219
TACCGCT2350.024.89265429
AGCCCAC2300.024.45557417
CCGAGCC2400.024.37405614
ATGCCGT2350.023.93524444
CGTATGC2500.023.39909641
GCGACCC500.002265574222.4991321
GCGCGCT500.002265574222.4991311
GCCCGCA500.002265574222.4991330
ACGGGCA602.4688488E-422.49912816
GTATGCC2600.022.49912842
CGGGACC602.4688488E-422.49912816
CCGCTGA2800.021.6955931
Produced by FastQC (version 0.11.2)