FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_4_66.3410000000b119.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_4_66.3410000000b119.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences539390
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTC15890.29459203915534216RNA PCR Primer, Index 20 (95% over 21bp)
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA7780.1442370084725338No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT2150.038.71861637
CACGAGA2150.038.71861621
TATGCCG2200.037.84215543
CTCGTAT2200.037.8386539
TGCCGTC2250.037.0012245
CATCTCG2250.036.9977936
ACGAGAC2250.036.9977922
CCCACGA2300.036.1934919
TCGTATG2300.036.1934940
CGTATGC2450.034.89587441
AGCCCAC2400.034.6854317
ATGCCGT2450.033.98071344
CGAGCCC2450.033.97756215
CCACGAG2450.033.97756220
GGACATC2450.033.0592533
GCCCACG2550.032.64510718
GTCCGAT352.8193937E-432.14093828
CCGAGCC2600.032.0173214
GAGCCCA2600.032.0173216
CCTGTGG2550.031.76280828
Produced by FastQC (version 0.11.2)