FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_4_65.3410000000b10c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_4_65.3410000000b10c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences982779
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC13500.13736557252444345No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACAG301.1400365E-437.49868814
TGCCGTC1400.036.96299445
CGCGGCT250.002107322435.9987438
GCCCACG1600.032.34261718
CGTATGC1650.031.36253741
GGACTCC300.00514778129.998956
GTCGTCC300.00514778129.9989526
CACGTGG300.00514778129.9989516
TATGCCG1750.029.5703943
CCCACGA1750.029.5703919
ATGCCGT1750.029.5703944
CGAGCCC1800.028.74899315
GCGACGA406.166766E-428.1240149
GAGCCCA1850.027.97199216
AGCCCAC2000.026.99905417
CCGAGCC1950.026.5375314
ACGAGAC1950.026.5375322
GTATCAA23750.026.3775231
CGGGCTT608.192195E-626.2490817
CCACGAG2000.025.87409420
Produced by FastQC (version 0.11.2)