FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_4_64.3410000000b0fe.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_4_64.3410000000b0fe.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1175420
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGTC67850.5772404757448402No Hit
ACTTAGATGTTTCAGTTCGCTAAGTTTTCAAAGTCCAAAGAGCTGTCTCTT15250.12974085858671794No Hit
CTCTTTGGACTTTGAAAACTTAGCGAACTGAAACATCTAAGTCTGTCTCTT14430.1227646288135305No Hit
GTATAGATTCTATCCTCGGAAGGAAACAAGAATAGAAGAAGAACCTCTAAT12460.10600466216331182No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC8400.038.57022545
CCCACGA8900.036.15055519
GCCCACG8950.035.6972118
CGAGCCC9000.035.24890515
CCGAGCC9150.034.91694314
CCACGAG9250.034.782720
ATGCCGT9300.034.59569544
GAGCCCA9300.034.35376716
TATGCCG9450.034.28464543
AGCCCAC9300.034.1118417
CGAGACC9550.033.92564423
ACGAGAC9650.033.5740822
TCCGAGC9700.032.70516213
GTATGCC9900.032.49898542
ATCTCGT10350.031.7381437
CATCTCG10150.031.69851936
TCTCGTA10400.031.15287438
GACATCT10350.031.08598734
CACGAGA10400.030.93653521
GAGACCA10450.030.78851324
Produced by FastQC (version 0.11.2)