##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23V3BCXX l02n01 kb2_4_62.3410000000b0d4.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 46 Sequences flagged as poor quality 0 Sequence length 51 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.0 0.0 0.0 0.0 0.0 0.0 2 35.630434782608695 0.0 0.0 0.0 0.0 0.0 3 34.95652173913044 0.0 0.0 0.0 0.0 0.0 4 35.17391304347826 0.0 0.0 0.0 0.0 0.0 5 35.69565217391305 0.0 0.0 0.0 0.0 0.0 6 36.19565217391305 0.0 0.0 0.0 0.0 0.0 7 35.73913043478261 0.0 0.0 0.0 0.0 0.0 8 37.02173913043478 0.0 0.0 0.0 0.0 0.0 9 36.28260869565217 0.0 0.0 0.0 0.0 0.0 10 36.45652173913044 0.0 0.0 0.0 0.0 0.0 11 36.43478260869565 0.0 0.0 0.0 0.0 0.0 12 36.43478260869565 0.0 0.0 0.0 0.0 0.0 13 37.69565217391305 0.0 0.0 0.0 0.0 0.0 14 36.891304347826086 0.0 0.0 0.0 0.0 0.0 15 36.891304347826086 0.0 0.0 0.0 0.0 0.0 16 37.02173913043478 0.0 0.0 0.0 0.0 0.0 17 36.23913043478261 0.0 0.0 0.0 0.0 0.0 18 36.21739130434783 0.0 0.0 0.0 0.0 0.0 19 36.608695652173914 0.0 0.0 0.0 0.0 0.0 20 36.5 0.0 0.0 0.0 0.0 0.0 21 36.56521739130435 0.0 0.0 0.0 0.0 0.0 22 35.73913043478261 0.0 0.0 0.0 0.0 0.0 23 37.30434782608695 0.0 0.0 0.0 0.0 0.0 24 37.95652173913044 0.0 0.0 0.0 0.0 0.0 25 35.41304347826087 0.0 0.0 0.0 0.0 0.0 26 36.17391304347826 0.0 0.0 0.0 0.0 0.0 27 37.34782608695652 0.0 0.0 0.0 0.0 0.0 28 36.19565217391305 0.0 0.0 0.0 0.0 0.0 29 37.02173913043478 0.0 0.0 0.0 0.0 0.0 30 35.82608695652174 0.0 0.0 0.0 0.0 0.0 31 36.19565217391305 0.0 0.0 0.0 0.0 0.0 32 35.41304347826087 0.0 0.0 0.0 0.0 0.0 33 36.08695652173913 0.0 0.0 0.0 0.0 0.0 34 36.34782608695652 0.0 0.0 0.0 0.0 0.0 35 35.47826086956522 0.0 0.0 0.0 0.0 0.0 36 36.30434782608695 0.0 0.0 0.0 0.0 0.0 37 35.56521739130435 0.0 0.0 0.0 0.0 0.0 38 34.91304347826087 0.0 0.0 0.0 0.0 0.0 39 34.369565217391305 0.0 0.0 0.0 0.0 0.0 40 36.34782608695652 0.0 0.0 0.0 0.0 0.0 41 34.108695652173914 0.0 0.0 0.0 0.0 0.0 42 34.15217391304348 0.0 0.0 0.0 0.0 0.0 43 34.608695652173914 0.0 0.0 0.0 0.0 0.0 44 35.5 0.0 0.0 0.0 0.0 0.0 45 37.369565217391305 0.0 0.0 0.0 0.0 0.0 46 37.02173913043478 0.0 0.0 0.0 0.0 0.0 47 36.80434782608695 0.0 0.0 0.0 0.0 0.0 48 35.08695652173913 0.0 0.0 0.0 0.0 0.0 49 35.28260869565217 0.0 0.0 0.0 0.0 0.0 50 34.52173913043478 0.0 0.0 0.0 0.0 0.0 51 35.52173913043478 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2206 1 7.75 2206 2 4.25 2206 3 1.0 2206 4 7.0 2206 5 4.25 2206 6 0.0 2206 7 -0.5 2206 8 -5.0 2206 9 -0.5 2206 10 -0.5 2206 11 1.5 2206 12 9.25 2206 13 -1.75 2206 14 2.75 2206 15 4.5 2206 16 4.25 2206 17 1.5 2206 18 1.5 2206 19 1.5 2206 20 7.75 2206 21 -4.0 2206 22 9.0 2206 23 -4.5 2206 24 1.5 2206 25 3.25 2206 26 9.0 2206 27 -0.25 2206 28 3.25 2206 29 -4.5 2206 30 -8.25 2206 31 4.5 2206 32 6.25 2206 33 4.25 2206 34 -5.5 2206 35 -13.0 2206 36 3.0 2206 37 -3.0 2206 38 -4.5 2206 39 2.75 2206 40 2.75 2206 41 1.5 2206 42 6.25 2206 43 11.75 2206 44 10.5 2206 45 5.75 2206 46 4.5 2206 47 3.0 2206 48 4.5 2206 49 11.75 2206 50 -3.0 2206 51 1.5 1115 1 7.75 1115 2 -1.75 1115 3 -4.0 1115 4 -4.0 1115 5 4.25 1115 6 0.0 1115 7 1.5 1115 8 3.0 1115 9 1.5 1115 10 -0.5 1115 11 -4.5 1115 12 1.25 1115 13 4.25 1115 14 2.75 1115 15 -1.5 1115 16 -6.75 1115 17 1.5 1115 18 -4.5 1115 19 -4.5 1115 20 -17.25 1115 21 -4.0 1115 22 -2.0 1115 23 1.5 1115 24 -4.5 1115 25 3.25 1115 26 -16.0 1115 27 -5.25 1115 28 -15.75 1115 29 1.5 1115 30 2.75 1115 31 -1.5 1115 32 -18.75 1115 33 -6.75 1115 34 -5.5 1115 35 6.0 1115 36 3.0 1115 37 3.0 1115 38 1.5 1115 39 -16.25 1115 40 -8.25 1115 41 1.5 1115 42 -18.75 1115 43 0.75 1115 44 8.5 1115 45 3.75 1115 46 -3.5 1115 47 1.0 1115 48 -1.5 1115 49 0.75 1115 50 3.0 1115 51 1.5 1210 1 1.75 1210 2 4.25 1210 3 7.0 1210 4 1.0 1210 5 -1.75 1210 6 0.0 1210 7 -0.5 1210 8 1.0 1210 9 -0.5 1210 10 1.5 1210 11 1.5 1210 12 -17.75 1210 13 -6.75 1210 14 -8.25 1210 15 -1.5 1210 16 4.25 1210 17 1.5 1210 18 1.5 1210 19 1.5 1210 20 7.75 1210 21 7.0 1210 22 9.0 1210 23 1.5 1210 24 1.5 1210 25 9.25 1210 26 9.0 1210 27 5.75 1210 28 9.25 1210 29 1.5 1210 30 2.75 1210 31 -1.5 1210 32 6.25 1210 33 -1.75 1210 34 5.5 1210 35 1.0 1210 36 -3.0 1210 37 3.0 1210 38 1.5 1210 39 10.75 1210 40 2.75 1210 41 1.5 1210 42 6.25 1210 43 0.75 1210 44 -2.5 1210 45 -2.25 1210 46 2.5 1210 47 1.0 1210 48 -1.5 1210 49 -13.25 1210 50 -3.0 1210 51 1.5 2109 1 -17.25 2109 2 -6.75 2109 3 -4.0 2109 4 -4.0 2109 5 -6.75 2109 6 0.0 2109 7 -0.5 2109 8 1.0 2109 9 -0.5 2109 10 -0.5 2109 11 1.5 2109 12 7.25 2109 13 4.25 2109 14 2.75 2109 15 -1.5 2109 16 -1.75 2109 17 -4.5 2109 18 1.5 2109 19 1.5 2109 20 1.75 2109 21 1.0 2109 22 -16.0 2109 23 1.5 2109 24 1.5 2109 25 -15.75 2109 26 -2.0 2109 27 -0.25 2109 28 3.25 2109 29 1.5 2109 30 2.75 2109 31 -1.5 2109 32 6.25 2109 33 4.25 2109 34 5.5 2109 35 6.0 2109 36 -3.0 2109 37 -3.0 2109 38 1.5 2109 39 2.75 2109 40 2.75 2109 41 -4.5 2109 42 6.25 2109 43 -13.25 2109 44 -16.5 2109 45 -7.25 2109 46 -3.5 2109 47 -5.0 2109 48 -1.5 2109 49 0.75 2109 50 3.0 2109 51 -4.5 >>END_MODULE >>Per sequence quality scores pass #Quality Count 28 1.0 29 1.0 30 2.0 31 3.0 32 2.0 33 1.0 34 5.0 35 7.0 36 3.0 37 5.0 38 3.0 39 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.130434782608695 19.565217391304348 8.695652173913043 32.608695652173914 2 6.521739130434782 28.26086956521739 58.69565217391305 6.521739130434782 3 10.869565217391305 32.608695652173914 32.608695652173914 23.91304347826087 4 13.043478260869565 23.91304347826087 32.608695652173914 30.434782608695656 5 10.869565217391305 34.78260869565217 41.30434782608695 13.043478260869565 6 28.26086956521739 47.82608695652174 19.565217391304348 4.3478260869565215 7 23.91304347826087 30.434782608695656 23.91304347826087 21.73913043478261 8 19.565217391304348 50.0 19.565217391304348 10.869565217391305 9 17.391304347826086 21.73913043478261 32.608695652173914 28.26086956521739 10 8.695652173913043 43.47826086956522 30.434782608695656 17.391304347826086 11 28.26086956521739 41.30434782608695 21.73913043478261 8.695652173913043 12 23.91304347826087 28.26086956521739 28.26086956521739 19.565217391304348 13 28.26086956521739 32.608695652173914 23.91304347826087 15.217391304347828 14 19.565217391304348 21.73913043478261 34.78260869565217 23.91304347826087 15 8.695652173913043 17.391304347826086 43.47826086956522 30.434782608695656 16 17.391304347826086 43.47826086956522 19.565217391304348 19.565217391304348 17 13.043478260869565 34.78260869565217 34.78260869565217 17.391304347826086 18 17.391304347826086 21.73913043478261 32.608695652173914 28.26086956521739 19 10.869565217391305 26.08695652173913 43.47826086956522 19.565217391304348 20 19.565217391304348 23.91304347826087 34.78260869565217 21.73913043478261 21 15.217391304347828 26.08695652173913 36.95652173913043 21.73913043478261 22 21.73913043478261 39.130434782608695 19.565217391304348 19.565217391304348 23 17.391304347826086 28.26086956521739 26.08695652173913 28.26086956521739 24 17.391304347826086 23.91304347826087 23.91304347826087 34.78260869565217 25 10.869565217391305 34.78260869565217 34.78260869565217 19.565217391304348 26 19.565217391304348 36.95652173913043 28.26086956521739 15.217391304347828 27 17.391304347826086 32.608695652173914 26.08695652173913 23.91304347826087 28 10.869565217391305 39.130434782608695 28.26086956521739 21.73913043478261 29 21.73913043478261 26.08695652173913 23.91304347826087 28.26086956521739 30 10.869565217391305 39.130434782608695 32.608695652173914 17.391304347826086 31 21.73913043478261 28.26086956521739 30.434782608695656 19.565217391304348 32 15.217391304347828 43.47826086956522 30.434782608695656 10.869565217391305 33 17.391304347826086 30.434782608695656 34.78260869565217 17.391304347826086 34 17.391304347826086 26.08695652173913 30.434782608695656 26.08695652173913 35 17.391304347826086 23.91304347826087 34.78260869565217 23.91304347826087 36 13.043478260869565 32.608695652173914 34.78260869565217 19.565217391304348 37 13.043478260869565 26.08695652173913 30.434782608695656 30.434782608695656 38 28.26086956521739 28.26086956521739 30.434782608695656 13.043478260869565 39 19.565217391304348 21.73913043478261 34.78260869565217 23.91304347826087 40 17.391304347826086 23.91304347826087 45.65217391304348 13.043478260869565 41 32.608695652173914 15.217391304347828 36.95652173913043 15.217391304347828 42 21.73913043478261 28.26086956521739 28.26086956521739 21.73913043478261 43 13.043478260869565 17.391304347826086 39.130434782608695 30.434782608695656 44 17.391304347826086 28.26086956521739 26.08695652173913 28.26086956521739 45 4.3478260869565215 32.608695652173914 34.78260869565217 28.26086956521739 46 17.391304347826086 36.95652173913043 28.26086956521739 17.391304347826086 47 8.695652173913043 28.26086956521739 32.608695652173914 30.434782608695656 48 19.565217391304348 23.91304347826087 21.73913043478261 34.78260869565217 49 19.565217391304348 23.91304347826087 30.434782608695656 26.08695652173913 50 15.217391304347828 26.08695652173913 34.78260869565217 23.91304347826087 51 17.391304347826086 26.08695652173913 39.130434782608695 17.391304347826086 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.5 27 1.0 28 1.0 29 1.0 30 3.0 31 5.0 32 3.5 33 2.0 34 6.5 35 11.0 36 9.0 37 7.0 38 6.0 39 5.0 40 4.0 41 3.0 42 2.5 43 2.0 44 1.0 45 0.0 46 0.0 47 0.0 48 1.5 49 3.0 50 2.5 51 2.0 52 3.0 53 4.0 54 2.0 55 0.0 56 0.0 57 0.0 58 0.0 59 0.0 60 0.0 61 0.0 62 0.0 63 0.0 64 0.0 65 0.0 66 0.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 46.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 100.0 #Duplication Level Percentage of deduplicated Percentage of total 1 100.0 100.0 2 0.0 0.0 3 0.0 0.0 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GACGAATATAGGACCTCTTAAATCTTACAAAACATTTGAAACCAATCAGTC 1 2.1739130434782608 No Hit GTGCTACAACAGGGAAATGAGCCAAATGCAATCCTACTGTGACGAGTCCTG 1 2.1739130434782608 No Hit ATATTGAAGAGGCTTTTCATGAGCAAAAGTCAACAAAGCTGCTCTTTCTCT 1 2.1739130434782608 No Hit CTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTC 1 2.1739130434782608 No Hit GGATTCTTCCATGGTTACTCCTTCATCACATTACTCATGATTCTTAACCAT 1 2.1739130434782608 No Hit GTATCGTCTACGATTAACATCGAATCTTGAAGATTTGTGTTCTGCTGATAT 1 2.1739130434782608 No Hit GTCAGATTTCACGAGAGTCATAGCAGCTCCAAATTTATCTATAACAGGCAA 1 2.1739130434782608 No Hit TTACAGAGATTTGGCAGTTGCTTCTCCTCACTCTAACTTAGAGCAAATAAA 1 2.1739130434782608 No Hit TTCCGATACTTCTTGAAATCTATCCTCCTCATATGCTGTCTCTTATACACA 1 2.1739130434782608 No Hit ACACTACAGAGAGGTGACAAAGAGGGATTATGCTTCAGAAAGCGAATGGAA 1 2.1739130434782608 No Hit GGGGAAGTTTTATCACTCATTAATCATGGAGATGAAACTGGTGAAGAAATA 1 2.1739130434782608 No Hit TTGTAGGCATATACTACGGAATATATACGATAGAGACCTGTCTCTTATACA 1 2.1739130434782608 No Hit GGACTTCTTGAACTTCTCTACCTCTAAGTAGACAGTTGAGTAACTGTCTCT 1 2.1739130434782608 No Hit CTCTAAAACAAATCCAAAATTTTGAACACAAGCATACATGCATGCAGCTGT 1 2.1739130434782608 No Hit CATGTATATAACCTATTGTAACGACTCTGTGTAGAACTTTATATTCAGACT 1 2.1739130434782608 No Hit CTCCATTGAAGCTCTCTCTTTGCCTTTTTTTTTTTTTTTTTCCCCAAATAA 1 2.1739130434782608 No Hit TTCTTTTATCAAATCTCAGGAACATGGTCTAATCATGAGGGTCTGTCTCTT 1 2.1739130434782608 No Hit GATTAAGCCATGCATGTGTAAGTATGAACGAATTCAGACTGTGAACTGTCT 1 2.1739130434782608 No Hit CATCTACACCATTCTCTTTGTTCAAAGAGATATGTTTATCCTCACTAGCAT 1 2.1739130434782608 No Hit CATTCATAGAGCTTAATATCCATCTTAACTCGGAAATACTTAGCCCCTGTC 1 2.1739130434782608 No Hit CAGTAGGGTAGTAGCAAAGTTTCTCAATAAACACCTTCTTAAATAACATCC 1 2.1739130434782608 No Hit AATAGGGTCTAGATCACTTGGGAGCAACCTTTTCCCCCCGTACTCTGCGTT 1 2.1739130434782608 No Hit AATGAAGGCTGTTTTCTTTTTCTTTTTTTTTTGTTGTTGTTGGCTATGATG 1 2.1739130434782608 No Hit CTACCGCAGATCTGGACGAATCCTTTTCTTCATACAAATGTGTAAAAGATG 1 2.1739130434782608 No Hit CCCGAGAAGTTAAAGAAACTGGACCTGATGGTGTGGAGGTGACCAAAGTTG 1 2.1739130434782608 No Hit ATCAAAGAAGATGTATTGACCTCCTCCACAAATCCAAGGAGGAGAAAACCT 1 2.1739130434782608 No Hit GTTCAACATAAGATCCACAACATCCAAAAACAACTTCCACTGTCTCTTATA 1 2.1739130434782608 No Hit ATAATGAATCATCATCTCTCTCACAAAACTCTAATACTGTCTCTTATACAC 1 2.1739130434782608 No Hit GTTACAAGATATGATAAACGAAGTAGATGCTGATGGTACTGTCTCTTATAC 1 2.1739130434782608 No Hit ACTTTTGTCTGAACTTGTTTCAGTTAAGTTATTCCTTGTTGTTGGATTGTT 1 2.1739130434782608 No Hit GAAAGAACCTGCAATGGATGTAACACCAACGACGACCGCAGAGACGCCAAG 1 2.1739130434782608 No Hit GAAATGGAATGAGAAGCTAGAGAGGTGATTAAGTGGGACTTTTATACCTGT 1 2.1739130434782608 No Hit CTACTTAGATGTTTCAGTTCGCTAAGTTTTCAAAGTCCAAAGAGCGCCTGT 1 2.1739130434782608 No Hit CTCCGATTGATAGTTGTTTTTGGAGCCAAAGCTTCAAGTACCATTATTCTT 1 2.1739130434782608 No Hit CTTCTCCAGACTACAATTCGAACGCCGAAGACGTCCGATTTTCAAGCTGGG 1 2.1739130434782608 No Hit CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC 1 2.1739130434782608 No Hit GAATTTGATATAGTCCTCCCTCATCATTATGTTTGTTTAAGATACCAAGAA 1 2.1739130434782608 No Hit GAGTTTATTCAGCTTCCTATTGATTTGCAATGGACCTGTCTCTTATAAACA 1 2.1739130434782608 No Hit CTAGCAATTTAGCAAAATACAATCAATCACTCAACTCAAAGATTATCCCCG 1 2.1739130434782608 No Hit ATCCATAACAGAGACGAATTTACTTGTACGCAACAACATAGCTGTCTCTTA 1 2.1739130434782608 No Hit GTATCAACGCAGAGTACGGGGTGGTATCACTGTCTCTTATACACATCTCCG 1 2.1739130434782608 No Hit GTATAGCATGCAAGCAACCTAACCGAGTGACTTGATGACCTGCCCCCCTGT 1 2.1739130434782608 No Hit AATCAAAGAAGAATCATTTTGAGATACAAAGAACAACAACTGTCTCTTATA 1 2.1739130434782608 No Hit GTTTTGAGTTCTAGTTGATTTCATGTTGGTTATGTTCTGTCTCTTATACAC 1 2.1739130434782608 No Hit CTCAAGGACAATGATATCATTTCAAAGAGTTAGAACAGAGTATACACCTGT 1 2.1739130434782608 No Hit GTTATTTGAGAGGCTTACCAAGAAACCACCTTCGCTTGTTGATCTTTGCGT 1 2.1739130434782608 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.0 22 0.0 0.0 0.0 23 0.0 0.0 0.0 24 0.0 0.0 0.0 25 0.0 0.0 0.0 26 0.0 0.0 0.0 27 0.0 0.0 0.0 28 0.0 0.0 0.0 29 0.0 0.0 0.0 30 0.0 0.0 2.1739130434782608 31 0.0 0.0 2.1739130434782608 32 0.0 0.0 2.1739130434782608 33 0.0 0.0 2.1739130434782608 34 0.0 0.0 2.1739130434782608 35 0.0 0.0 2.1739130434782608 36 0.0 0.0 6.521739130434782 37 0.0 0.0 10.869565217391305 38 0.0 0.0 13.043478260869565 39 0.0 0.0 15.217391304347826 >>END_MODULE >>Kmer Content pass >>END_MODULE