FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_4_62.3410000000b0d4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_4_62.3410000000b0d4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46
Sequences flagged as poor quality0
Sequence length51
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGAATATAGGACCTCTTAAATCTTACAAAACATTTGAAACCAATCAGTC12.1739130434782608No Hit
GTGCTACAACAGGGAAATGAGCCAAATGCAATCCTACTGTGACGAGTCCTG12.1739130434782608No Hit
ATATTGAAGAGGCTTTTCATGAGCAAAAGTCAACAAAGCTGCTCTTTCTCT12.1739130434782608No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTC12.1739130434782608No Hit
GGATTCTTCCATGGTTACTCCTTCATCACATTACTCATGATTCTTAACCAT12.1739130434782608No Hit
GTATCGTCTACGATTAACATCGAATCTTGAAGATTTGTGTTCTGCTGATAT12.1739130434782608No Hit
GTCAGATTTCACGAGAGTCATAGCAGCTCCAAATTTATCTATAACAGGCAA12.1739130434782608No Hit
TTACAGAGATTTGGCAGTTGCTTCTCCTCACTCTAACTTAGAGCAAATAAA12.1739130434782608No Hit
TTCCGATACTTCTTGAAATCTATCCTCCTCATATGCTGTCTCTTATACACA12.1739130434782608No Hit
ACACTACAGAGAGGTGACAAAGAGGGATTATGCTTCAGAAAGCGAATGGAA12.1739130434782608No Hit
GGGGAAGTTTTATCACTCATTAATCATGGAGATGAAACTGGTGAAGAAATA12.1739130434782608No Hit
TTGTAGGCATATACTACGGAATATATACGATAGAGACCTGTCTCTTATACA12.1739130434782608No Hit
GGACTTCTTGAACTTCTCTACCTCTAAGTAGACAGTTGAGTAACTGTCTCT12.1739130434782608No Hit
CTCTAAAACAAATCCAAAATTTTGAACACAAGCATACATGCATGCAGCTGT12.1739130434782608No Hit
CATGTATATAACCTATTGTAACGACTCTGTGTAGAACTTTATATTCAGACT12.1739130434782608No Hit
CTCCATTGAAGCTCTCTCTTTGCCTTTTTTTTTTTTTTTTTCCCCAAATAA12.1739130434782608No Hit
TTCTTTTATCAAATCTCAGGAACATGGTCTAATCATGAGGGTCTGTCTCTT12.1739130434782608No Hit
GATTAAGCCATGCATGTGTAAGTATGAACGAATTCAGACTGTGAACTGTCT12.1739130434782608No Hit
CATCTACACCATTCTCTTTGTTCAAAGAGATATGTTTATCCTCACTAGCAT12.1739130434782608No Hit
CATTCATAGAGCTTAATATCCATCTTAACTCGGAAATACTTAGCCCCTGTC12.1739130434782608No Hit
CAGTAGGGTAGTAGCAAAGTTTCTCAATAAACACCTTCTTAAATAACATCC12.1739130434782608No Hit
AATAGGGTCTAGATCACTTGGGAGCAACCTTTTCCCCCCGTACTCTGCGTT12.1739130434782608No Hit
AATGAAGGCTGTTTTCTTTTTCTTTTTTTTTTGTTGTTGTTGGCTATGATG12.1739130434782608No Hit
CTACCGCAGATCTGGACGAATCCTTTTCTTCATACAAATGTGTAAAAGATG12.1739130434782608No Hit
CCCGAGAAGTTAAAGAAACTGGACCTGATGGTGTGGAGGTGACCAAAGTTG12.1739130434782608No Hit
ATCAAAGAAGATGTATTGACCTCCTCCACAAATCCAAGGAGGAGAAAACCT12.1739130434782608No Hit
GTTCAACATAAGATCCACAACATCCAAAAACAACTTCCACTGTCTCTTATA12.1739130434782608No Hit
ATAATGAATCATCATCTCTCTCACAAAACTCTAATACTGTCTCTTATACAC12.1739130434782608No Hit
GTTACAAGATATGATAAACGAAGTAGATGCTGATGGTACTGTCTCTTATAC12.1739130434782608No Hit
ACTTTTGTCTGAACTTGTTTCAGTTAAGTTATTCCTTGTTGTTGGATTGTT12.1739130434782608No Hit
GAAAGAACCTGCAATGGATGTAACACCAACGACGACCGCAGAGACGCCAAG12.1739130434782608No Hit
GAAATGGAATGAGAAGCTAGAGAGGTGATTAAGTGGGACTTTTATACCTGT12.1739130434782608No Hit
CTACTTAGATGTTTCAGTTCGCTAAGTTTTCAAAGTCCAAAGAGCGCCTGT12.1739130434782608No Hit
CTCCGATTGATAGTTGTTTTTGGAGCCAAAGCTTCAAGTACCATTATTCTT12.1739130434782608No Hit
CTTCTCCAGACTACAATTCGAACGCCGAAGACGTCCGATTTTCAAGCTGGG12.1739130434782608No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC12.1739130434782608No Hit
GAATTTGATATAGTCCTCCCTCATCATTATGTTTGTTTAAGATACCAAGAA12.1739130434782608No Hit
GAGTTTATTCAGCTTCCTATTGATTTGCAATGGACCTGTCTCTTATAAACA12.1739130434782608No Hit
CTAGCAATTTAGCAAAATACAATCAATCACTCAACTCAAAGATTATCCCCG12.1739130434782608No Hit
ATCCATAACAGAGACGAATTTACTTGTACGCAACAACATAGCTGTCTCTTA12.1739130434782608No Hit
GTATCAACGCAGAGTACGGGGTGGTATCACTGTCTCTTATACACATCTCCG12.1739130434782608No Hit
GTATAGCATGCAAGCAACCTAACCGAGTGACTTGATGACCTGCCCCCCTGT12.1739130434782608No Hit
AATCAAAGAAGAATCATTTTGAGATACAAAGAACAACAACTGTCTCTTATA12.1739130434782608No Hit
GTTTTGAGTTCTAGTTGATTTCATGTTGGTTATGTTCTGTCTCTTATACAC12.1739130434782608No Hit
CTCAAGGACAATGATATCATTTCAAAGAGTTAGAACAGAGTATACACCTGT12.1739130434782608No Hit
GTTATTTGAGAGGCTTACCAAGAAACCACCTTCGCTTGTTGATCTTTGCGT12.1739130434782608No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers