FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_4_60.3410000000b0ae.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_4_60.3410000000b0ae.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences985001
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTC20880.21197948022387791No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACG2900.033.36086718
CTCGACG3100.031.20855128
ACGGCAT3150.030.71317732
ATGCCGT3150.030.71317744
TCGACGG3150.030.71317729
TATGCCG3200.030.23328443
CCCACGA3300.029.99891719
TGCCGTC3450.028.69461845
CTCGTAT3300.028.6353339
CGTATGC3450.028.04246741
GTATGCC3450.028.04246742
GCCTCGA1750.026.99902732
GGCGCGT755.639413E-826.9990237
CGGGGCA1850.026.7557917
TCGTATG3650.026.50589240
CGTCGGG1700.026.46963314
CCGAGCC3700.026.14770514
GACGGCA3700.026.14770531
GGCATCT3700.026.14770534
TACCCGG1650.025.9081556
Produced by FastQC (version 0.11.2)