FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_4_58.3410000000b084.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_4_58.3410000000b084.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1109687
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTC43670.3935343930315485No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC5500.039.6803945
ATGCCGT6400.034.4518844
GCCCACG6700.032.57345218
CCCACGA6750.032.3321719
TATGCCG6850.031.86016543
CGAGCCC6950.031.40174915
CCGAGCC7100.031.05521814
AGCCCAC7050.030.95633117
GTATGCC7450.029.29424742
CTGGACC7550.029.20424530
CCACGAG7650.029.11659620
CGAGACT7750.028.740923
CGTATGC7600.028.71607241
TCCGAGC7750.028.45058613
CTCGTAT7600.028.42002939
ACTCTGG7900.027.62558727
TCGTATG7950.027.45184140
CATCTCG7900.027.34078836
TCTGGAC8300.026.29423329
GGACCAT8500.026.20493932
Produced by FastQC (version 0.11.2)