FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_4_54.3410000000b041.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_4_54.3410000000b041.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences760161
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTC13870.1824613470041215RNA PCR Primer, Index 20 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC2150.036.62683545
GCCCACG2400.034.6864818
CCCACGA2550.033.5284319
CGAGCCC2450.033.06025715
CCGAGCC2500.032.3990514
TATGCCG2600.031.15293543
ATGCCGT2550.030.8814544
CGTATGC2650.030.56514441
CCCCCAT300.00514691229.9991228
CCACGAG2900.029.48189520
AGCCCAC2750.029.45368417
GAGCCCA2850.028.42022116
TCCGAGC2900.027.93021813
CGAGACC3000.026.9992123
CCTGTGG3100.026.85405328
GTATGCC3050.026.55660242
CACGAGA3250.026.30692321
CTCGTAT3200.025.31176239
CTCCGAG3300.025.22653612
ACCTGTG3550.024.08380327
Produced by FastQC (version 0.11.2)