FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_4_52.3410000000b027.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_4_52.3410000000b027.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1182143
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGTC162311.3730149398169256No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGT20610.1743443898073245No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC18450.043.1691545
ATGCCGT20900.038.216344
GCCCACG21150.037.87095318
CCGAGCG301.1401877E-437.49862316
CCGAGCC21600.037.29030214
CGAGCCC21500.037.2544515
CGTATGC21550.037.06360641
TATGCCG21550.036.959243
AGCCCAC21700.036.9110917
CCCACGA21700.036.9110919
CGAGACC21800.036.8449823
GTATGCC21750.036.61934742
CCACGAG21950.036.59319320
GAGCCCA22200.036.18110716
CATCTCG22150.036.161236
ACGAGAC22350.036.03894822
GGCGGGT250.002107511735.9986822
TCCGAGC22700.035.58239413
TCGTATG22550.035.5197640
CACGAGA23100.035.0636521
Produced by FastQC (version 0.11.2)