FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_4_50.3410000000b00d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_4_50.3410000000b00d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCCAGGAACTGTGTTGATCATTCTTGCTGGTAGATTCAAGGGTAAGAGA21.5384615384615385No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21.5384615384615385No Hit
GGTGAAAAGTACCCCGGGAGGGGAGTGAAATAGTTCCTGAAACCATGTGCT10.7692307692307693No Hit
ACATTCCATCAGGCCAAATTGTACATCCTGTAATAACAGCTGTCTCTTATA10.7692307692307693No Hit
GCTTTTTGCATGTACCTTATGAATAAGGACCGGCTAATTCCGTGCCAGCAG10.7692307692307693No Hit
GTTATATAAGATGAGAACCAGATTATAGCATCGTGACTTAAACTCAGAAGA10.7692307692307693No Hit
CCTCAATCGCCATATCTCAAAATGCAAGTATCCTCAGCATGGCAGCTCTTA10.7692307692307693No Hit
ATCCTTAGCTGGTCTGAGAGGATGATCAGCCTGTCTCTTATACACATCTCC10.7692307692307693No Hit
CTTTGACGGCGTTGGCCAGGATGTTCACACCCTCGACCATGCGATGGCGCG10.7692307692307693No Hit
CATCTACAAGGTCTGGAATTACTTTCAGTCTTTCACGAAACATCACCGATA10.7692307692307693No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA10.7692307692307693No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA10.7692307692307693No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.7692307692307693No Hit
GGTGAGCGAGAGAACTCTCGTTAAGGAACTCGGCAAAATGACCCCGTAACT10.7692307692307693No Hit
GTATTGGTCTTATAGAGAGAAGCTAACAAAAAGTATTAAGCTGTCTCTTAT10.7692307692307693No Hit
GTGGAGCCGTTCCTCAGCTGGTACTCCATTGAACCTTTCGATTAAAACATT10.7692307692307693No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAATACTT10.7692307692307693No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA10.7692307692307693No Hit
GTATCAACGCAGAGTACGGGCCGTATAGACCGTACCCGAAACCGACACAGG10.7692307692307693No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC10.7692307692307693No Hit
GTCATGCGTATCTAAGCGTTGTCTATTCTACTTTTCATCCTTGTTACTGTC10.7692307692307693No Hit
CCCTGAAGGCTTCCTTAAGCTCTTCCTCTGAATCAGTGTCTTTCATCTTAC10.7692307692307693No Hit
GCTGTAACAGGAACTAAAGCTTTAGAAGCTGTTCAAAAAGCTGACAGCGAT10.7692307692307693No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTATACCC10.7692307692307693No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACCC10.7692307692307693No Hit
GTTTGAAAGAGGTTTTTGCTGACGTGTCGTTAACGGTTTCGAACGACGTTA10.7692307692307693No Hit
GAAGGTGAAGTCTTCTAAGAAGAAGAAGAAGAAGGATAAGGACTGTCTCTT10.7692307692307693No Hit
ACCAACTGGAGGACCGCACCGGTAAGCGTTGAAAAGCTTTCGGATGAACTG10.7692307692307693No Hit
GCTGTACGGATTTACCAATACAGCGGGCCACACGCTTACACCACAATCCAA10.7692307692307693No Hit
GTCTTTGATTATCGGTTTCATACGACAAAAAAAAAAAAGAATTATCGGCAT10.7692307692307693No Hit
GTCGAAGGAACTCGGCAAAATGACCCCGTAACTTCGGGAGAAGGGGTGCTC10.7692307692307693No Hit
GTATCAACGCAGAGTACGGGGGAAAAGATTGATGATGAATACAGGGCTAAT10.7692307692307693No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAACTGTCTCTTA10.7692307692307693No Hit
CTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTATTAAACAGTC10.7692307692307693No Hit
GCGAAAGGCCATGGGGAGATACCGTCTGTGATCCATGGATCTCCGACTGTC10.7692307692307693No Hit
GTACCTCAGGAACGTCAATGGACGTTCATGTCCCTTCATCAAAATCGGCCT10.7692307692307693No Hit
ATTCCGGCCAGTGCCAAAGACGTGCTGGAAAAAATCAAGGAAGCGCTGGCG10.7692307692307693No Hit
CAACGCAGAGTACGGGGAAATAGCTCGTACTCCCCGAAATGCCTTTAGGGG10.7692307692307693No Hit
ACGTGAACTAGCGTCCACGCTTCAAAGCCTCCCACCTATCCTACACCTGTC10.7692307692307693No Hit
GAATACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAATGGTTTATCCACCG10.7692307692307693No Hit
GTTAAAGGCTTGGACGTCGTGAAGGCGATTGAGAAAGTTGGATCTGATTCT10.7692307692307693No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.7692307692307693No Hit
CTCTACCCGTACCGCAAACCGACACAGGTAGTCGAGGCGAGTAGCCTCAGG10.7692307692307693No Hit
ATTCAGGGCACCGGACAGGTCGGTCTTGACACTGTCTCTTATAACATCTCC10.7692307692307693No Hit
GTATCAACGCAGAGTAAGGGTGGGTGCGGTGTCAACTGCTGGGTCACGAGG10.7692307692307693No Hit
TATTTAATGAAGGCAAGCGTTCAATTTACATTTATATACATTAGTATTTAA10.7692307692307693No Hit
GGTATCAACGCAGAGTACTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.7692307692307693No Hit
CTGTTGGTGCATGCCCTGCACGTAGCCCAGCACGGTCTGTGCGCGCTGGGG10.7692307692307693No Hit
TTTTTTCCCAGGGCTACTCGGTATTTTGCTTGCACGGGTCGCCAAGGCTAT10.7692307692307693No Hit
TCTCTGTTGGTTTCTTATTTCCTTTTATCCCCAAGCTCCTGTCTCTTATAC10.7692307692307693No Hit
CAACTAAAAACTTAAAACAACACCAGAGATAACGATCTGTCTCTTATACAC10.7692307692307693No Hit
CAGGTACACCAGCGGTACGTCCACTCCGGTCCTCTCGTACTCTGTCTCTTA10.7692307692307693No Hit
CGGGTGTCCAATGCCATGTGGTGTTCAGATAACAAGAATGGAACATATCAA10.7692307692307693No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTGTGTTGTAACGCCCCTA10.7692307692307693No Hit
GATCGAGACTTCGTCGAGCTATGATCCGATTTCGTTAGTTTAGCATCTGTC10.7692307692307693No Hit
GGTTTCATATATCTCACTGATGACGATGTTATAAGGCATCCGCTGTCTCTT10.7692307692307693No Hit
AAGCTCAGTCTCAGGAGGAAGCGGAGCAGTCCAAATGTTTTTGAGATGGCA10.7692307692307693No Hit
GACATCGGGTGCTAACGTCCGGTGTCAAGAGGGAAACAACCCAGACCGCCA10.7692307692307693No Hit
GCGTTGGCCGGCGCATTGATGGCAATGCGCAGCACGATTGGCCCCTTTGAT10.7692307692307693No Hit
GTTGTGATGTGATCTTGGATACTCCACAAAACAACTGACTTGTCCTTGCCC10.7692307692307693No Hit
GTGTAATGATGGGGCCAACCGAAGTTCCTGCTCAGTCACCTGAAACTACTA10.7692307692307693No Hit
GTATCAACGCAGAGTACGGGTCAGCCTGCGACTCCCCGTCTTTTTTCCCGA10.7692307692307693No Hit
GTACCAGACTGTGCCTGCTGGATCACATCAAGACCCTTGCAAAAGGGTACA10.7692307692307693No Hit
CTATTAAATTTTAACACCCATGTTTTGATTCAAAAGAAGAGAGGGAGATCT10.7692307692307693No Hit
CACCCAAAGCACTCAAAATTGAGCTTAACTTAACATTCAGACAAAAATTTA10.7692307692307693No Hit
GTACAATGTTTAATTTCCTGGAAATTCCTATATAGTCATTATGTGCTATTG10.7692307692307693No Hit
TTCGAACCTTTTCATCAAACACTGCCTTGGCCAAAGTCTTGAATCCTGTCA10.7692307692307693No Hit
CACTATTGGTGTTGAATTCGCCACCAGAACTACTCAGGTTGAAGGAAAGAC10.7692307692307693No Hit
CCCGAAGGCCTTCATCCTGCACGCGGCATTGCTGGATCAGGCTTTCGCCCA10.7692307692307693No Hit
ATAGGGCGCCGAGCATCACGAGCCGATAGGCACCTCTTTCCGTACACACTA10.7692307692307693No Hit
CTCTCTGTATTCACTCAAGCTCTTCTCTACACTGTAAATTGTTGTATCAAC10.7692307692307693No Hit
TTGTTGTGGTCAATTAGTACCAGTAGCCTTCACACCTTACAGTGCTTCCAG10.7692307692307693No Hit
TCTCATTAGTGTTCTTTACGTTGAATATCGAACTCGTAAAGAACTGTCTCT10.7692307692307693No Hit
CTCTGGTTCACTACCCGTTATCTGTTATTTAAGAGGAACAGAGAAGAACTC10.7692307692307693No Hit
CCGTTCTTTAATCCAGTAGCTCCCGAATTTCGGCTTTGGCGATTCCGGGTC10.7692307692307693No Hit
ACATTGGTCATTCTTGTCTCATTCACCCACCTTTTGAAGGAAAATGATTGA10.7692307692307693No Hit
GGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAATACTCTGGGT10.7692307692307693No Hit
GGTATCAACGCAGAGTACGGGGGAGAGAAGACGAAACACAAACTGTCTCTT10.7692307692307693No Hit
ATCCTTAATGAATCAAGCGAGTTCGTCGGAGACAAGGTTGCCTATGCACCT10.7692307692307693No Hit
CCTTCTTCCTTTGCTAAAGATACAAAGACAAGGAGTTATTTGCCATAAACT10.7692307692307693No Hit
GAAGAAGGAGATGTATTTCCCAGTGAGGAAGTACGCTATCAAAGTGTAGTT10.7692307692307693No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTTCCCCCCCCTTC10.7692307692307693No Hit
ACTCGAGTTGTTATGTTTTTTCACTGATTCCATGCGCAGTCATGAGAGTTT10.7692307692307693No Hit
GTTTTGTTGTATGCGATTTTGACTTAGTTTATTTCTTGAATTCGACTTTAC10.7692307692307693No Hit
TCAGAGGCTAAGCAAGTACTCTTATGTCTACTGTGTTGATCTTGTCTGTTT10.7692307692307693No Hit
CTTTTGACCCTTCACCTTTGCCGGCCGTGCCACAATCAAGGTGAAGAGTAC10.7692307692307693No Hit
GTCTTTAAGAAAACCAATTCAACATTCCCTGAAAAAGAACTGTCTCTTATA10.7692307692307693No Hit
GTGGTATGTGGATGACGGATGATTGCAAGAAATCGTTCATGGAGATGAAAT10.7692307692307693No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA10.7692307692307693No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA10.7692307692307693No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC10.7692307692307693No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG10.7692307692307693No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTGTTTTTACCCCCCCCTC10.7692307692307693No Hit
TTGATAGGCTGGGTGTGGAAGAGCAGTAATGCGTTAAGCTGACCAGTACTA10.7692307692307693No Hit
ATGTGGTACAAATATCGGGTGAGAGTTATCGTTTGAAGGATAAAAAAAGAA10.7692307692307693No Hit
GTCTCAGCTGATGACAGTGTGTATGCAAATACTGAAGATGTACCTGAAATG10.7692307692307693No Hit
GATCTGTGTAGGATAGGTGGGAGGCTTTGAAGCGTGGACGCTAGTTCACGT10.7692307692307693No Hit
TATTATGATGCTACATTAATGGATTTAGGTTTTTTGTTTTTTGTTTTTTTT10.7692307692307693No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTCAGTT10.7692307692307693No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA10.7692307692307693No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTGCTTTTATACC10.7692307692307693No Hit
TTGGTAAGTAGTAAGAACCCTAGCTTTAGATTCGTCAGATTCTGATTACTC10.7692307692307693No Hit
CTTACAAGATGCGAGCGGTCAGAGCGCGGGGTTGTTGTAGCAGTGCCATGT10.7692307692307693No Hit
GGTGACGGCGGTTGTGGAGCCTTCACTGGCTGCAGCGAAGCCAGACCAGAA10.7692307692307693No Hit
GATCGAGAAGGGCGTTGCCTTCGAGACCATCCCCGTCGATCTCATGAAAGG10.7692307692307693No Hit
TATCCAAACTTTAAAAAAAATCAATGTTATCTTTTTGTTTTTTGTCATTGT10.7692307692307693No Hit
GTACGGGCAATTAGTACTGGTTAGCTTAACGCATTACTGCGCTTCCACACC10.7692307692307693No Hit
CATACAAACAGTCGGAGCCTGGAAACGGGTGACGGCGTACCTTTTGTATAA10.7692307692307693No Hit
GTACTGTGTTCGCTATCGGTCATGTACGAGTATTTAGGCTTGGAGGGTGGT10.7692307692307693No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCCC10.7692307692307693No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATG10.7692307692307693No Hit
CTTTTGTATAATGGGTCAGCGACTTACATTCAGTGGCAAGCCTGTCTCTTA10.7692307692307693No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTGCGTTTATACCCCTCCCCC10.7692307692307693No Hit
GTTCTTGAGTTTCATCTCTCTGGAGACTCTGTCCCACACAACTGCTTCTCC10.7692307692307693No Hit
ATCACGAATCGCGTTACAAACATCAAGAGCTTTAACACTTCTCTCGAAGTA10.7692307692307693No Hit
GATAAAACTACAGCTCCTGAGAAATGATAATGGCGATTCCTGTCTCTTATA10.7692307692307693No Hit
ATTCACTTCCGTGCTCCCTCCAAGATCTTCTGGCGTACCGTTCGCGGTATG10.7692307692307693No Hit
GACCGAGGGTGATGCCGGGGCCGGGGTAGGTGCCGTCCATGATCGAATTCA10.7692307692307693No Hit
CTTCTGATATCTCCAGGTCTTGTCACCAAAAACCTTCCCCGATTGACAGAA10.7692307692307693No Hit
GCTGACCGACCGCATGCTGCTCGGCTCGGGCGACACGGTGCACGAGCGCCT10.7692307692307693No Hit
GTGAAAAGCACCCCGGGAGGGGAGTGAAAGAGTACCTGAAACCGTGTGCCT10.7692307692307693No Hit
GTGCAGGATCGACACCCAGCCCGCGGCCGGGAGTCGGTAGGTCGTGAGGTC10.7692307692307693No Hit
ATAAAGTATATAGCACATTTTCACTTTTCCCATTATTTATTTTTATTCCAC10.7692307692307693No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGTTAATCCCCCCCTTCC10.7692307692307693No Hit
ATTAATGGATTTAGGTTTTTTGTTTTTTGTTTTTTTTTCACCTAGGTTTTT10.7692307692307693No Hit
CTTCATACCTGAAGAAGGTCGGATACAACCCTGACAAAATCCCATTTGTGC10.7692307692307693No Hit
GAGGAGGAGGAGTTAGGAGGAGCAAGATGGCCTTTCTCTTGTAAGCTCTTG10.7692307692307693No Hit
CTTCTGCTTCTGAGAAGACACTGTCAAAACCTGAGCTATGGATTTACGCAC10.7692307692307693No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers