FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_4_5.3410000000aca6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_4_5.3410000000aca6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences860668
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGTCG207.0339075E-444.9979122
TGCCGTC850.037.05710245
TAATTCG300.005147719329.99860435
GCCCACG1100.028.63503318
TATGCCG1150.027.39003243
CCGAGCC1150.027.39003214
CGAGCCC1150.027.39003215
CCCACGA1200.026.24877719
ATGCCGT1251.8189894E-1225.19883244
CGCTCAT450.001226636724.99883730
CTGTGCG551.368244E-424.5443159
TACGGGC751.6975082E-623.99888415
CATCTCC9350.023.1005345
CCCCTGA500.00226476322.49895543
TAGACTA802.9745133E-622.4989555
TAAGCGG802.9745133E-622.49895516
CCACGAG1459.094947E-1221.7231320
CTCCGAG1501.4551915E-1120.99902312
CGAGACA1501.4551915E-1120.99902323
GAAGCGG654.2383684E-420.76826718
Produced by FastQC (version 0.11.2)