FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_4_49.3410000000aff3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_4_49.3410000000aff3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences251866
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGCGT300.00514060529.9989416
GTATTAG450.001212851825.0388871
CAGCGGC450.001224147924.99911932
AGCGGCT450.001224147924.99911933
GCGGCTG450.001224147924.99911934
CGGCTGG450.001224147924.99911935
GTGTAAT602.4343374E-422.5349981
GGTATCA1405.456968E-1222.5349961
AGCCGGA500.002260203722.49920516
AACGGCT702.6929174E-522.4992058
ACTACAC500.002260203722.4992053
GTATCAA3500.020.6034261
CAGCCGG550.00392894220.45382315
GGTAGGG550.00392894220.45382323
GTGCTAA600.006438656218.7791651
GTAGGGT600.006498038318.74933824
TAGGGTC600.006498038318.74933825
GCGCTTA600.006498038318.74933813
CTACACC851.2000324E-418.5287574
GTCTTAT800.001688183616.9012471
Produced by FastQC (version 0.11.2)