FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_4_46.3410000000afcc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_4_46.3410000000afcc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences837652
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACG601.546141E-1037.49865718
CGTATGC803.3942342E-928.12399341
CCCACGA856.4792403E-926.46963919
CGGGGCA957.421477E-1026.05169717
TATGCCG901.1905286E-824.99910543
ATACCCG952.1145752E-823.6833615
GTATGCC1052.3646862E-923.57058342
TACCCGG802.9740804E-622.4991956
TCGCGCG903.285877E-722.4991959
CGCGCGC1009.0520007E-720.24927510
GGGGCAA908.249415E-619.99928518
CGTCGGG908.249415E-619.99928514
ACTGCGC807.561951E-519.6867948
TCGTATG1151.5946534E-719.56451640
ATGCCGT1151.5946534E-719.56451644
CAGCGGG706.982986E-419.28502544
CAACGCA7650.018.8228555
GCGCGGC600.006510418.74932911
AACGCAG8150.018.4962716
GTCGCGC1102.2552667E-618.4084328
Produced by FastQC (version 0.11.2)