FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_4_41.3410000000af7c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_4_41.3410000000af7c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences732104
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA31760.43381814605575164No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT27210.37166850611388547No Hit
TACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTA23860.3259099800028411No Hit
CCGTTACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATG16080.2196409253330128No Hit
TTACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTT14250.1946444767410095No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC9340.12757750264989673No Hit
GGATTACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAA8090.11050342574279064No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCGCA207.0328166E-444.998439
GCCCCAA653.6743586E-1034.61417830
GTCTACG855.456968E-1231.8135721
ATGCCGT1150.031.30325544
GGGGCAT651.4026227E-831.15275826
CAGCCCC751.7025741E-929.99895328
CCGTTAC3550.029.199781
GGGGGCA702.8841896E-828.9275625
AGCCCCA803.3924152E-928.1240229
TAGGCCG406.165248E-428.1240229
GCGTATC406.165248E-428.124029
GCCCACG1300.027.69134118
CGAGTCG903.947207E-1027.499046
CTACGAG1004.5474735E-1126.9990563
CCCACGA1350.026.66573519
TATGCCG1400.025.71338843
AGGCCGC450.001226378724.99912830
CGTATGC1500.023.99916341
GCGGTAA851.892331E-723.82269941
TGCAGCC952.1134838E-823.68338426
Produced by FastQC (version 0.11.2)