FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_4_40.3410000000af6f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_4_40.3410000000af6f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1541
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGTC40.25957170668397145No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC30.1946787800129786No Hit
CCATATTGCCAAGGATTAAACTCGATCGATTTGAGGTCCGTTTCGTTGGAG20.12978585334198572No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC20.12978585334198572No Hit
CCATTTCGTGAACCCACTTTGAGTTTCCGAGCCAATTGCAGATGAGATCGT20.12978585334198572No Hit
ATCGTACCCTTCAAGCTCATCTTCTTGAGAAGATCAAATCTGATGATTGAT20.12978585334198572No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGT20.12978585334198572No Hit
CATGGATCCAGAGGTGGTGCAAGAAGAAAACTACATCAACAGATCAACCAA20.12978585334198572No Hit
CTTCTCGCCATGGATTGACCGTTGCTCGAACCTGGGCTTTCAGCGATGGCG20.12978585334198572No Hit
ATATTAAGTGTCTCCAACTACTGAGATCTCTATCCTCTCCGTCCTCTGTTC20.12978585334198572No Hit
GAGTTTCTTGATAAATTCAGGATTCGGAGATACAAGAGGCCTAAGATTTCT20.12978585334198572No Hit
CTCTGCTGCTAGCTCTGCTTGTGACCACATCCGTGACTGGGTCCTTGGAAC20.12978585334198572No Hit
CTCCAAGGGCGTTTGGTGGTTCAAATCTCTCTTCGGCAAATAGATTACACG20.12978585334198572No Hit
GTCGAGGATGAGGCCTATATTGGTACAAGTGCAACTGTCTTGGATCTGTCT20.12978585334198572No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)