FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_4_38.3410000000af46.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_4_38.3410000000af46.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACAAGTACCGCGAGGTAAAGATGAAAAGGACTTTGAAAAGAGAGTCAAAG21.639344262295082No Hit
AGCGACGTCTCCACAAGCGTATCAAAAGCCCGGGCTTAGGCCGCCACCGTA21.639344262295082No Hit
GCTCAAGGCAGGAAACGCGTACCACAAGTACCGTGTGAAGAGGAACTCATG10.819672131147541No Hit
ATGGAACAATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACTTCGGG10.819672131147541No Hit
CTTTGTTATCTCAATCAAGGCCAACTGTATCGACTCTACAGTTGCAGCAGA10.819672131147541No Hit
CACCAGAGGATATAAGCTAAGTTACTAAGAGAGCTGTCTCTTATACACATC10.819672131147541No Hit
CTGTTGCTGTTTGCCGCCGTCAAGAGCCATTTGCTTGGACATATCGGCAAG10.819672131147541No Hit
GAGGAGGAGGAGACCGATTCCGAGTGTTAAAAGGAAAACAGATTCGCCGCT10.819672131147541No Hit
GCCTGGCCCGGTCGCCCTACGCTACTGGCGCTTCAAAAGGCGAAAGTCTCG10.819672131147541No Hit
GGACCAAGGAGTCTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAA10.819672131147541No Hit
GTTCGGAACTGGGACCCCCGAGCCCAGCCCTCAGAGCCAATCCTTTTCCCG10.819672131147541No Hit
AGTAATTGCAGAACCTTTCATTTCTCCCTTGGGGTTGACAATGACTCCAGC10.819672131147541No Hit
CACTAGCTCTCTTCTTAGTGGTCTGGTACGTCAATTTACCTCCTGGAGTTT10.819672131147541No Hit
GTATAAAGATGAAGATGCAGCGTGGAGGGATAGAATAACCCACCCAGGGCC10.819672131147541No Hit
GAGTCAAAGAGTGCTTGAAATTGTCGGGAGGGAAGCGGATGGGGGCCGGCG10.819672131147541No Hit
GCAGAATGTAGTGTACGTTCATGAGGGAATTTGTAAGTTTATTTATTGTAG10.819672131147541No Hit
TGATAACCCAAAGGTTTAGCAATCTCGAGAGTAAAAGAAAGAAACCATAAA10.819672131147541No Hit
CCTCGGCATCAGCGTGCTCCGGGCGTCGGCCTGTGGGCTCCCCATTCGACC10.819672131147541No Hit
GTGCTGAGACGGAGTTTCTCAGATTGAGCTTTTGTTGAAGGAAGTGAAAGG10.819672131147541No Hit
GTTTCTCAATCTGTGGATCTGGACCATCCTCTTGCACTCAACTTTCTACGG10.819672131147541No Hit
CCCTCGCGGGTGCACCGCCGACCGACCTTGATCTTCTGAGAAGGGTTCGAG10.819672131147541No Hit
GACCAGGACGCATCGCCGGCCCCCATCCGCTTCCCTCCCGACAATTTCAAG10.819672131147541No Hit
CAATTGTTGGTCTTCAACGAGGAATTCCTAGTAAGCGCGAGTCATCAGCTC10.819672131147541No Hit
CCCATATAGGTGGGCTCTAAAGATCACTCCGGTTCTTCATATGTGAGCATA10.819672131147541No Hit
AGTTGAAGATTACTTTTCATGTGGATCTCATACATTATGTCTGTCTCTTAT10.819672131147541No Hit
GACGTGTCGGCTGCTTTGTTGAGCCGTCACACGGAATCGAGAGCCCCAAGT10.819672131147541No Hit
GTCCGGGCACGACGGGGCTCTCACCCTCTCTGGCGCCCCTTTCCAGGGAAC10.819672131147541No Hit
TCCAAAAAGTGATACAACAAGGTTTAGGATTTGTTGTTCCTAAAGTACATC10.819672131147541No Hit
GTGTTGCTCACTATACTCCTTGGCTTATCTATTGGTGGAACACACAAAAAT10.819672131147541No Hit
GTCTTAATCGACCAACACCCTTTGTGGGTTCTAGGTTAGCGCGCAGTTGGG10.819672131147541No Hit
TTACTTGCCACCACCAACTTCCTTGACGGCACAGATCTGCTCCTCTCCCAT10.819672131147541No Hit
GTTAGATTTCGGGTTGGAGAGAGATCTCAGCTTAGAGTTTTCGAGCTACGA10.819672131147541No Hit
TCGTTATTACGGAGGTAACGAATTCATCGATGAGATCGAGAATCTTTGCCG10.819672131147541No Hit
GTATGGGCAAAGGCCAATGATTCCTCCTCCTGGTGGTATGATGAGAGGACC10.819672131147541No Hit
CCAAAGAAACTTCACAGTTAGATCATCATCAGATCAAGAAAACGAGACTTA10.819672131147541No Hit
GTATCATCTTGCCAGATCTCATGGAATTTGAACCAAAAGAAACTATAATTA10.819672131147541No Hit
CCCAAAGAGTGGAGTTATAATTGAGGAGATAGAAGATGAAGAGAAACCTGC10.819672131147541No Hit
CTTGTTCGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAATTTACC10.819672131147541No Hit
CTCTCCATCGATCCGATTCGCTTGTTGGCGATTTTTGAAGAGTGAAACGGA10.819672131147541No Hit
GGAGATATCAGAAGTGAGAATGCTGACATGAGTAACGATAAATCCCTGTCT10.819672131147541No Hit
GTTTTGAACAATCTATACACATGTTCAGGAAGAGTTTGGACCTGTCTCTAT10.819672131147541No Hit
GTTGTGCGGCTCTGCGAATCTTCGAAAATGGGTGCGTACAAGTATGTATCT10.819672131147541No Hit
AATGGGGAGCCCACAGGCCGACGCCCGGAGCACGCTGATGCCGAGGCACGC10.819672131147541No Hit
AATATGAACCTTGAGTCATCAAAGCCTTTCTGACTCGGCCTGGCCCGGGTC10.819672131147541No Hit
CCTAAATAGTTTTCGGAGAGAACCAACTATTTCCAAGTTTGTTTAGCCTTT10.819672131147541No Hit
CATCTATCCCTAAGCATATCACATGCACAGTTGTAGAAACACAACTTCCAA10.819672131147541No Hit
ATGCAAGAACATCGAGCTGGGATCCAAGTTTCTCCTCTGCAACCTTCTCGG10.819672131147541No Hit
ACGCAGAGTACGGGGGTGTAACCCTAGGCCTAGGATCGCACCACGCACACG10.819672131147541No Hit
GTCTTGGAGTTGGTGCCTTCCGTAGGATCTATGGAGGAAGCAAGAGGAACG10.819672131147541No Hit
GTATGGAGCCAGTTGAGAAGTGTCTTCGTGATGCTAAGATGGACAAGAGCA10.819672131147541No Hit
AACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA10.819672131147541No Hit
GGCATCGAGTGATCGACGAGCAGCCTCGGCTATACGGATGAACGGGACCTC10.819672131147541No Hit
CCATTAACAGTTGGACCTGTATCAGCTGTGCTTTCTGTGGATTCTGTCTCT10.819672131147541No Hit
ATTGAAACCACTACAGCAACGTAGTTCTTGTAATTTCCTTCAACAATGACA10.819672131147541No Hit
TCTTAAGAGTATGTACAATGCAGAGAAGAGAGGAAAGAGGCAGGTTATGAT10.819672131147541No Hit
CTCGTTTGTTGATATCTGAAAATTTCTCAATCGTCCAAGTATTTTTCCCAA10.819672131147541No Hit
AAACACGATCGCCTGCCAGTGGAAGGCTTTCTGCGTTCAGTCAATCTACGC10.819672131147541No Hit
GGAGAGGCGAGTGGAATTCCGAGTGTAGAGGTAAAATTCGTAGATATTCGG10.819672131147541No Hit
CCTCTGTGACGGTTGGCTCTCTTCCTTGGAAGGAAACCAGGAGAACCATGT10.819672131147541No Hit
CTAGTTGAAGATTACTTTTCATGTGGATCTCATCTGTCTCTTATACACATC10.819672131147541No Hit
ATTTTAACCGGATCCCCTTTCGAAGTTCGCGCTTAAGCGCTATCAGACGGG10.819672131147541No Hit
CCCAAAGCTTTTGGGATTCAAGAGCAGCAGCAAATCCATCAAACCCTGCGT10.819672131147541No Hit
CTGCAACTCCCATGCACAGAACCTTCTTCAGCTGGAACTTCACTGTTGCCT10.819672131147541No Hit
GGTTTGAGCATTGTATCAAGAGTGAAAAGTACAAGCCATCACATTCATCCT10.819672131147541No Hit
CTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGAC10.819672131147541No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC10.819672131147541No Hit
GTATGAAGAGATAAGTCCTCCTAAAGTGGATGATTTTTGTTACATTACGGA10.819672131147541No Hit
AAATATATGAATCGGAGGCTGAATTTACATTCTGTCTCTTATACACATCTA10.819672131147541No Hit
AGTCAATACACAAACGAACTAGATAATAGAAATCAAACCACCAGCAAGAGA10.819672131147541No Hit
GTTCTGGTCTGCCCAGATGGAAAGACGATTGAAGCTGAAGCGGCCCATGGA10.819672131147541No Hit
ATATACACTCAGTGCTGTACAAATCTTGGCCCTATTTGACAAAATCAACAT10.819672131147541No Hit
GCAAGTTTCAATGGCCTTAACAACAACACACTTACAAAATGACTTGTAAAT10.819672131147541No Hit
ACCAAAGACAGGAGGGTGCTTGCAAAGTATACTAGGTTGCCTGCGTCGCCT10.819672131147541No Hit
CTTTCATTATGTGCCTCCTCCTGGATTTTGATCTTCTTTCTCAAGTTAAAA10.819672131147541No Hit
CCAATAACATTAGCCGCCGTCATAACTTTCGTTTGCAACCTTCGTCGGAAA10.819672131147541No Hit
TCTCCGGACTCCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAAT10.819672131147541No Hit
ACTTGTGATATGTTATTATTTAACTGGTTATCATAAGGAAACTCGAAGTTG10.819672131147541No Hit
CAACTTAATTAACTCCCAAAATAACAATGGTTCTCAAAAGTAAAAATTTCA10.819672131147541No Hit
GTTCAAAAGGCGTTCCTTCTTTGCGGCTTTGTCCTCTGGCCTATACCTGTC10.819672131147541No Hit
GTACGGGAAAAAGCAAAATTAATAAATTATCGTCTTGTCGAAAAATCGTAG10.819672131147541No Hit
GTTATGAGTACGACCGGGCGTGAGCGGCACTCGGTCCTCCGGATTTTCAAG10.819672131147541No Hit
GTTGAGGATAACTCCTTCATACTTAACTTGGAACCACTTCATGGCATCATC10.819672131147541No Hit
GAACTATGCCTGAGCGGGGTAAAGCCAGAGGAAACTCTGGTGGAAGCCCGC10.819672131147541No Hit
TCGCAGACTCATTGAGATTGAACCAGAGAATGCAGGGAACTATGTCATCCT10.819672131147541No Hit
CTCTCTGCGTGCCCTAGTTATTCAGATCGCCGAATTTGCAGTAAGGAGACG10.819672131147541No Hit
GACATATGCCGACGAGAGAGAAGAAGAAGTGAAGCTATTAGAAGGATCAGT10.819672131147541No Hit
GCATTGCAAAGGTTGTCAAAAGCATGCTTTAGATGAGGAGTTACCACTTTG10.819672131147541No Hit
GTGAATTTCAGCATTCGCTGTCAATTTGCTAGTCTGCCCGTACTCTGCGTT10.819672131147541No Hit
TGCTAACGCAATGTGATTTCTGCCCGTACTCTGCGTTGACTGTCTCTTATA10.819672131147541No Hit
TCATTGTCTTGTGTTGAAGACAAGATGAAAACAAGTTGTTTGGTTAATGGT10.819672131147541No Hit
GTACCTTGACTGAAGATTCCTAAAGGGCGCTGTGAACTGTAATTGTGAAAA10.819672131147541No Hit
GATCTTGGGACCAGCACCACCTGATGCAGGAGGAGCTTCGTCCTCGTCCAT10.819672131147541No Hit
GCCCATGACGGCGTCGACAAACGCGGAAGAATATAGGAAGCTGTCTCTTAT10.819672131147541No Hit
GCCCCCGGTTTACCCTCCTACCAAAGCTCCGGTTAAGCCTCCGGTTTCTCC10.819672131147541No Hit
GGCTTTCGGTGGTATGATCTTCGTCCTCGTCTACTGCACCGCCGGTATCTC10.819672131147541No Hit
GAGTAAAGAGGCCAAAGTCATCTCCTCCACCAGTTAAATCTGACCTGGTTT10.819672131147541No Hit
GTTTAGTGGAATTATACTTTGTCTGGTCCATACGAAATAACTTATTCCTGT10.819672131147541No Hit
ACATTAATCTGCCTTTGTCTGTAGGAAGAAGCTCTCCTTCTTGGAATTTGG10.819672131147541No Hit
GAAGAGGTCACTCTTGGGTTGTACTTCATCTTCGCCGCCGGGATTGAGAGC10.819672131147541No Hit
GCTCCGTTCCGCATCCGACCAGGACGCATCGCCGGCCCCCATCCGCTTCCC10.819672131147541No Hit
GCATTGTGTGCTGGGTCAACCAAGATGATCTCGTAGTACTTGTAGGTCGAA10.819672131147541No Hit
CCCTATAGGTAACGGAGCCAAAACCGGGACGTGAGAGTCACGGGCACTTCT10.819672131147541No Hit
ATCCACTAGTGACAACAACCTCATCTTCGTAACACAATCTTCATTGGAAAG10.819672131147541No Hit
CATTTGTTCATGAGACGAGTCCATATAAGAGTCTTGAAGAACTCTGGGACC10.819672131147541No Hit
GACTTTTAAGTAAGCCACATATCGAACGAAAGTGGTAACATCACAAAGATG10.819672131147541No Hit
ACCACGAACAGCCTCTTCCAGTTTCTTCATGTCGGTTTCATCATCCCATGG10.819672131147541No Hit
TACACACGCAGATTAGAGCTCTCTCTCTCCCTTTCCAAGCTTGTTACCGAC10.819672131147541No Hit
ACGTTTTGGCCCCTGTAGTATGTTTTTGTGTGAACTACCTGAACATCTGTC10.819672131147541No Hit
GTTCCACAGATTGCAGTTGAAAAGCCAAATGGTAGTTTAGAAGCTGTCTCT10.819672131147541No Hit
CAACATAATCTTCCAGTTACAACGAATGTTCAGGTAGTTCACACAAAAACA10.819672131147541No Hit
CTCCGATGAAGTAACATCGAGACGATTCACAAGCTGTCTCTTATACAATCT10.819672131147541No Hit
GTCTAAAAAAGATGATCGAATACAAACTAAACAACCTGTCTCTTATAACAT10.819672131147541No Hit
GTCTAAACCCAGCTCACGTTCCCTATTGGTGGGTGAACAATCCAACACCTG10.819672131147541No Hit
CTTCTGATATCTCCAGGTCTTGTCACCAAAAACCTTCCCCGATTGACAGAA10.819672131147541No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.819672131147541No Hit
ATCCCAGGTCGAGGCGCTTTCCTCAGAGAAAGCAACCATATTGTTTAGAAT10.819672131147541No Hit
GAAGAAAGAAGAATCAGACGAAGAAGACTATGAGCTGTCTCTTATCACACT10.819672131147541No Hit
ATACTGACGTGCAAATCGTTCGTCTGACTTGGGTATAGGGGCGAAAGACTA10.819672131147541No Hit
GTCTTGTTGAAATCAACAGGGCCTACCATCTCCGCAAGACCAACAAATTGC10.819672131147541No Hit
CTTGTTGCTGTCTCGAGTTTCTGCTGAATAAACCGGCTTCCATACTGATCC10.819672131147541No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers