FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_4_30.3410000000aecd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_4_30.3410000000aecd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences926192
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTC18310.19769119145922226RNA PCR Primer, Index 20 (95% over 21bp)
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA13920.1502928118575846No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT10960.11833399554304076No Hit
TACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTA10860.11725430580268453No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCA552.750312E-936.8162445
CCCACGA2300.036.1937419
TGCCGTC2300.036.1937445
GGGACCG250.00210744435.99809631
TATGCCG2350.035.42366443
GCCCACG2400.034.6856718
CGTATGC2450.033.97779541
ATGCCGT2450.033.97779544
GGACATC2400.033.7482233
AGCCCAC2600.032.0175417
GTATGCC2600.032.0175442
CCTGTGG2550.031.7630328
CCGAGCC2700.031.66499914
TCCGAGC2700.030.83170913
AATCGAC300.005148076529.99841744
CCACGAG2850.029.20898420
CGAGACC2800.028.92704423
GAGCCCA2900.028.7053816
CGAGCCC2900.028.7053815
ACCTGTG2850.028.41955227
Produced by FastQC (version 0.11.2)