FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_4_3.3410000000ac62.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_4_3.3410000000ac62.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC31.9867549668874174No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGT21.3245033112582782No Hit
TTACTACAGGTTAACTATGAGGCACTTGTATTTTTGACGGATCTTTTGCTT10.6622516556291391No Hit
CATTTAAAGAGATAGCCCACTGGTGCATACGTGTACAACTATCTCTTATAC10.6622516556291391No Hit
ATATTGTTATCCAAAGAACCAAACACTCCTCTAAATCTCCTCAAACTAGTT10.6622516556291391No Hit
ATATTAGACATCCACACCATTAACATTACAAACGACAAAACCTTAAGAATG10.6622516556291391No Hit
CTCCTTCTTTGAAGCCATGTTTGCTATTAGACATCTTCTAATTATTTCTCG10.6622516556291391No Hit
GAATCATCCCATCCTTCTTCATCAAGTTGTGACCATATTAAGTACATTCAC10.6622516556291391No Hit
CAACAAAACCATTATCATATGTTTCATAGCAGAGAACATAAGGTCTGTCTC10.6622516556291391No Hit
GTACTATGTAGACTCACAAACTGTTTTGAGATGTCATACGAGCTGTCTTTT10.6622516556291391No Hit
TCATTAGATAATTAACAAGTCAACCGAAAGAAAACAGATTACAAATCACTT10.6622516556291391No Hit
CCGTTTTAGCTATGAATCAATCTGCTTTTTGATATTTTGTAGCTGTCTCTT10.6622516556291391No Hit
GTTTTGATATCATGTGCTAGTCTTGCTTTAGCCTGTCTCTTATACACATCT10.6622516556291391No Hit
CTACAAAATATCAAAAAGCAGATTGATTCATAGCTAAAACGGCTGTCTCTT10.6622516556291391No Hit
ATGTTCTCTCTTGGTATGAGAGATCTTGTACTTGTACAGAGATCTTTAACC10.6622516556291391No Hit
CTTCTTATCTGCCTTGAGCTTTTGTCTTACAACATGTTTTGAGTTTTCAGT10.6622516556291391No Hit
CCAGAAGAGTTACCGTTAAACAAAGCTGGTTTGTGTTTTGTTTTTCAATTT10.6622516556291391No Hit
ACTTTGTATTGAACTAAAAACATGGTCTGTAATGCAGTTGACCTGTCTCTT10.6622516556291391No Hit
CGTTGATTCACAGCATTTAATTTCTTCCATACAGGATCTTTTTCTAGTTCT10.6622516556291391No Hit
GTTACATACAGATTAGAACAAGGATGTTGTTTCTGTCTCTTTACACTCTCC10.6622516556291391No Hit
CTCTTTTAGGACTAACAGAAAAAAAAACGTTTGGTTTGGTATGATGGATTT10.6622516556291391No Hit
GTTATAACACGTACAGATACCAATCACACAGTATAGATACTGTCTCTTATA10.6622516556291391No Hit
TATATGACTTGCATGAGTAAAACTACCAGATAACGTTCGAACTTTGCTAAG10.6622516556291391No Hit
CCTCGTGAGTGCGGCACGCACCTTTTTGTTTGTTTCGGAGCCGTTTTTGGC10.6622516556291391No Hit
CTTATCTGCAATGTGCTCTGTATATACTCTTCTTGATACCTTGTCTCTGCT10.6622516556291391No Hit
GGTATCAACGCAGAGTACGGGGAAAATCAAAGTAAGATAATAAGGTGACAA10.6622516556291391No Hit
CTCTGAAGAAGAAAAAGAAACTAGTTGTTTGAGTTGTCATTATTGTGTTCT10.6622516556291391No Hit
TTGCAAGAGCTGTCGTTGGTAATACCCCGTACTCCTGTCTCTTATAAACAT10.6622516556291391No Hit
ATATAGACATGGTCTCAAAAGAAACAGAAAAAAGGGACAAATTGGACAATA10.6622516556291391No Hit
CTCTTCTGAGATTGCACTATGTTATGTAACTATTGAATGTGAGGATCTTTG10.6622516556291391No Hit
CTAGTATTCCCACTCAAAGTTCCAAACAGTGTCCCATTACCATCCATAACA10.6622516556291391No Hit
TGTCGAAATAAAGAGAACTGTGAAGAAGACGTGAATTGTTTTTGTACACAA10.6622516556291391No Hit
AATGAGGATGGTAAGGAGAAGAAGGTTACTATTGACCTGTCTCTTATAACA10.6622516556291391No Hit
AATCAAGAGTGAAACAAAAAGAAAGCAAGTAGAGATGCTCACTATATGAAC10.6622516556291391No Hit
CTTCATGTATTGCCTCGATAACTTCAGTTTACGCGGTATTTTCTCTGGTTG10.6622516556291391No Hit
TCTGTCCTTCTTCTTGATACGATCCATCCTCGTTAACCCAAAAATGTGGGT10.6622516556291391No Hit
TTGCAGAACTAAGCGGAAAGGGAGTAACCACAATCATTGGAGGCTGTCTCT10.6622516556291391No Hit
GTGGTATCAACGCAGAGTACGGGGGTACCTTCGGTGCCCGAGTTGTAATTT10.6622516556291391No Hit
TTATAATTCTTCTTCACCGGAATGAGAAGGATACTTCAGTTCTGATACTCC10.6622516556291391No Hit
ACCTTAAACATTACTCCTTTCCTTCAAATGTATCACACCTGTCTCTTATCA10.6622516556291391No Hit
GGTTAACTAATCGATTATGAAAAGGAAAAAAGGGTAAAGTAACTTGACTGT10.6622516556291391No Hit
GTAAGATGTTGTACTGTCTTGTGCTTTACTGAGATAGATCTGTCTCTTATA10.6622516556291391No Hit
GATGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAGGGTGACTA10.6622516556291391Illumina PCR Primer Index 5 (96% over 28bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTT10.6622516556291391No Hit
CATATCCACAAAGCTCTGATGGACATTAATGAATCTCCTGTGTATGTTCTT10.6622516556291391No Hit
GTTTCATCCTAATATTTATGCAGATGGGAGTATCTGCTTGGACATTCTACA10.6622516556291391No Hit
CTCTTATATCTAGGCAAAGATTTTGGTTTTCTGATTTATGTTCTTCGTTTG10.6622516556291391No Hit
CCCTTATTGAATACGCTCACATTGAGCAATGGATCGATTTCTCCTCATTGG10.6622516556291391No Hit
ACATTCATGTTGCAGTTTATTGTTGAATTCAAGTTCTATGTTTTTGACCTG10.6622516556291391No Hit
AATCTGAAAAGGTCATTGGTTTTATTCAATTCTTGAACCATCTCGTTGATG10.6622516556291391No Hit
AAAAAGTACTCTGCGTTGATACCACGTACTCTGCGTTGATACCTGTCTCTT10.6622516556291391No Hit
GTAGAGTTAAGAAGATGCATCAAGTAACATATTCTGATGGCCTGTCTCTTA10.6622516556291391No Hit
CCTTAGTTTTGGGGTTCATATCGTTCATTTCTTTTTTCACGCTTGTTTACA10.6622516556291391No Hit
ACAGAATGGTTAACCACTTTGTCCAAGAGTTCAAGAGGAAGAGTAAGAAGG10.6622516556291391No Hit
GAGCATGTTGGTGTGAATGCGCCGGGAACGGCGAATAGGATTGTTTTCTTC10.6622516556291391No Hit
AAGGGAAACAGATTGAAAAGAGTTTGAAAAACACCATCATAGTTCTTAAAA10.6622516556291391No Hit
GATGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAATAGAAAAGAA10.6622516556291391Illumina PCR Primer Index 5 (96% over 28bp)
CTCTTCTTCAAAGTTCGATTTATCGATACAATTCTTTTTTCCTACTAGCAT10.6622516556291391No Hit
TTACAGTACAGAAAAATCAAACAAAGGGGCAAATAGGAATGCTGTCTCTTA10.6622516556291391No Hit
GTTATACTCTTCCCTTTAGAGAGCTGAAAGTTTTTTTGTTTTGCGAACCCA10.6622516556291391No Hit
GATGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAGAATAT10.6622516556291391Illumina PCR Primer Index 5 (96% over 28bp)
TCACAGTAGTCCTCTTGTATGTTCCAGAGGCTTTGGGTAACTGGGACATCT10.6622516556291391No Hit
ACCCTGGAGCAGGTCGTCCTTGCCGTAGGAGCACACGTGGCGCTTGCCACC10.6622516556291391No Hit
GTATTGTTTTGGCCATTCAAGCTCCAAATCTGAGTAAGAAACTAAAGAAGA10.6622516556291391No Hit
ATACACACCACAAAAACGTAGCTAAGAAGAACACAAGCAAATCATAAACCT10.6622516556291391No Hit
GAACATGTTAGCTAGAAATGTTTGTCAATCTACTTTTGTGATCAACAACTA10.6622516556291391No Hit
GATGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGTAACATATA10.6622516556291391Illumina PCR Primer Index 5 (96% over 28bp)
GCGTTGAACTTGTAATCGATTTATCGACATTGAACTAATTCTGTCTCTTAT10.6622516556291391No Hit
GAAGAGTATCGTGTCTTATGTTAATGCCGTTTTCTACTACATGAATGCTTC10.6622516556291391No Hit
GGTTTAAAAGATGATTTCACTTAGAGTCGTGGTGTAATTCCTGTCTCTTAT10.6622516556291391No Hit
TCTATATAATCCTTCTTCCTTTGCTCCCATCCTAAAGATCTTTCCTTCTGT10.6622516556291391No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTCTT10.6622516556291391No Hit
GCGTTTGCACTGATTGAACACGATGATGCCATGCCGGAGGTGGATAACAAG10.6622516556291391No Hit
GTTTCTGTCTTTTCGCTTAAATCCTAAGTACTTTTGTCTGAACTTGTTTTC10.6622516556291391No Hit
GTCTCTGATTGACTCTTTTTTGGGGTGGAATCGATGTTGAAGCCTGTCTCT10.6622516556291391No Hit
CATTGGAAAAGGAAGAGTGGCAATAAAACACATGCTAGGATCCGATGGCTG10.6622516556291391No Hit
GGATGAAGCTTTGGGGTAGAAATGAAGTATGCGCCAAGTCCAAGTTCTGGT10.6622516556291391No Hit
TCGCGCCACTGGCTTTTCAACCAAGCGCGATGACCAATTGTGCGAATCCTG10.6622516556291391No Hit
GTATCAACGCAGAGTACGGGGCGCAGCTAAACTTTATTGGAGAAACCCTGT10.6622516556291391No Hit
GCCCTCACTGCCGCAGAACATTTATTAGCATTCGGAGTTTGTCAGGAATTG10.6622516556291391No Hit
GGTTTAGGTGAGTCATTTTTGATTGAGGTCATTTAAATATCTGTCTCTTAT10.6622516556291391No Hit
CGCCAGTGGTGCTATGCCCACAATGGCCAGCTTGGCGGCTGTCTCTTATAC10.6622516556291391No Hit
CTTCTTTGTGGTTTGATACACCAATTTACCTCCTGTCTCTTATACACATCT10.6622516556291391No Hit
CAAAAGAAATAGTGTTTTAGCCACACAAAAACAAGCAAAAAAAAAAGCAAA10.6622516556291391No Hit
AAGAGTCTTCGTCTCAAGAGGAAACTAAAGACAAAGAGAATGAGAAGATTG10.6622516556291391No Hit
GCTCTAAAGATCGACTCATATCTCTCATAGAGAGAAAAGCGAGGGAAGAAA10.6622516556291391No Hit
CTTATATAATGGTCATATTGCAAAACACTTGAAGTCATCTTCTTGAGTTCT10.6622516556291391No Hit
CCCGAAAGGGGTAGTGGATGGACAATGGGTTAATATTCCCACTGTCTCTTA10.6622516556291391No Hit
ACAATATTCTGTCAAAATAACATTGATAAGCAACTAGTTCTTAAACCCGAG10.6622516556291391No Hit
GAAAAAGAGAATGGAAGCAGAAAAGATATGTTAGTGAAGGAAATTTCAGAG10.6622516556291391No Hit
CATTGTCTGCAAATAAGAAGACCAATTAAAGGGTTCTCTCCAAACCAAGAA10.6622516556291391No Hit
AGCAAGATCACTGTCACTGCAGATGGCCAATTCTCCAAGAGGTATTTGAAG10.6622516556291391No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTCCACGGTGTTAACTA10.6622516556291391No Hit
ATAGTGAGCTTTATTGTAAGATCAGTTAATGAAACAAGCTTTGTTCTTTTC10.6622516556291391No Hit
GTCCTCTACTGTTCTCTTCTTGGATGATAAGATTACGGGATCATCATAATC10.6622516556291391No Hit
AATGAGTACTTGAGCAGATCTTACATGAAACTTAGAATTAACACTGTCTCT10.6622516556291391No Hit
GAGAGAACAGATGATATTACTACACAATACCAGAAATAAAAGAGAAAAACT10.6622516556291391No Hit
ATAGTTGATACAAGACAAACATAAACACACATAATCCTGTCTCTTATACAC10.6622516556291391No Hit
GGCTATTGCCGATCGGGTGACAAACTGGTCAAATGTTGTCATAGCCTATGA10.6622516556291391No Hit
TCATGAACTAAAACAACACAACTACCATACTTAAGTAAGCCAAATCCAACA10.6622516556291391No Hit
CATAATGGATGATGAACGAGACATGATTTATCTTCCTAAAGGAATGCCAAC10.6622516556291391No Hit
CAAGCCCACCTCCTGGTTGTCATCGCTCGATCACTTCCTTTTCCACCTGTC10.6622516556291391No Hit
GGATAGGGTTACAAGTTACAACAGGTACATACCTGTCTCTTATACACATCT10.6622516556291391No Hit
ACCCCTAGTGATGTGGTGCCCATACCTCTTGTTATTGATGCTGTCTCTTAT10.6622516556291391No Hit
ATCAACGCAGAGTACGGGGGATCTGGGCGAGATGCTTCTTGTGGTCCGTCA10.6622516556291391No Hit
ATTTAAATATTCCCACAGTTCTCAATCAAATATGCTTTTTCAGTTTTCCAA10.6622516556291391No Hit
GCATAAGTAAATTCAAGATGCACTACAATGAAGAAAGTGAAAGGTCAAGAC10.6622516556291391No Hit
CAATAGTGAAGGAAGCAATACACAAGTTATGGAGCAAAATAAAAGCTGTCT10.6622516556291391No Hit
GATGTGATCTCGTATGCCGACTTCTGCTTGAAAAAAAAAATTTTACATTAG10.6622516556291391Illumina PCR Primer Index 9 (96% over 25bp)
CCTTTTTTACTATTACTCCATGGGATTTTGTTCCTGTCTCTTATACACATT10.6622516556291391No Hit
GAGTTAATGGTTCTGTTTAGTTGGTTTCTTCTATTGAATCTTATGTTAGTA10.6622516556291391No Hit
GATGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAATTGTATACTT10.6622516556291391Illumina PCR Primer Index 5 (96% over 28bp)
TACTAGTCCCTCCTGTTCAGAAAGCAGTAATTACTATCTGTCTCTTATACA10.6622516556291391No Hit
GATAATAATCGGGCATAAGCATATTACCGAAGCTATGGATTTATAACTGTC10.6622516556291391No Hit
GCAATAAGGAATTTACATTTCTTGTGAAACTTGTGTTGCTGTCTCTTATAA10.6622516556291391No Hit
GCTTAGATGCCTTCAGCGGTTATCTCTTCCGTACTTAGCTACTCGGCAATG10.6622516556291391No Hit
GTAAGAAGAGGAAGCTTGTGAATGTTGATGTAGTTGAGAGCTGTCTCTTAT10.6622516556291391No Hit
GATGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACATAATGAGA10.6622516556291391Illumina PCR Primer Index 5 (96% over 28bp)
TCTTAAACGTCTTCATCCAATTTAGAGAGTCTCGTGTCCTAGAATCATCGT10.6622516556291391No Hit
AACCTGAGCTTCAGCGAATGGGCCAGCGTCTTCGGCGACGCCAAGATGACC10.6622516556291391No Hit
GAAATAAAACTTCTCTTGATAACCAGGTCCATCAGGAATATGAGACTTCCT10.6622516556291391No Hit
ATCATAGTGTAGGTTTTGATAAAAAGTTGGAGCGTTATGACGTTTTACCTG10.6622516556291391No Hit
GAGTTTAAGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGA10.6622516556291391Illumina PCR Primer Index 5 (96% over 27bp)
CGTGAAAACTACCCCTAAAAGCCAAAATGGGAAAGGAAAAGACTCATATCA10.6622516556291391No Hit
AACATATACATCCTTGATTAGGTTAACAACTGCTTAATCTCTGTCTCTTAT10.6622516556291391No Hit
GAGATGGAGGATTCTGCGTCAGCAGCGAAGCATCTGTCTCTTATACACATC10.6622516556291391No Hit
GTACACACGAATACATAACTGCAAAAGAGAGGTTCAATAATTCAAGAACTT10.6622516556291391No Hit
CCTATGTGACTGTGTAATGAAATGAAATTTGTCTCTATGTATGCTGACTCT10.6622516556291391No Hit
AGAACAAGGAACCTATCAGATGGGTAAGTTACTTAGGTACGGTAGAGAGCT10.6622516556291391No Hit
CTCCAACAAACTTCATATTTCTGCTCCTAGAACCAAAAGGATCCTAACCAT10.6622516556291391No Hit
TAGCAGAGAACATAAGGTATGATGCCTAGTTCATTCTCCTGTCTCTTATAC10.6622516556291391No Hit
AAATATAAAAGCCTTAAATTATCGTACAATGATGTCTCTGTCTCTTATACA10.6622516556291391No Hit
GTCTGAAATTATAAAGCTTTAATGCCTAGAATTTCTGGAATGATACCTTCA10.6622516556291391No Hit
AGAGTACTCATCATTTTGCTTGATCATGAGCAAATTGTCCCAATAATCTTT10.6622516556291391No Hit
CTCGGTTACAGATCCGATTTGGTTAACCTTCAAAAGAAGAGCATTGCAAGA10.6622516556291391No Hit
CTTTTTTTCATTGTTCGCATACAAAAAGTGAAACAGAATCTGTCTCTTATA10.6622516556291391No Hit
GGTTCATATCGTTCATTTCTTTTTTCACGCTTGTTTACAGTAATAGCTGTC10.6622516556291391No Hit
GATTTAGCATAAGTATGTCCCCTACAGAAGCATAGTTGGCATGCCCGTACT10.6622516556291391No Hit
GAAAAAACCAAATAAGCAAATATCGATAACAAAGCGGAAATTGCAGAATTC10.6622516556291391No Hit
CTCCAGTAATGCCCTTCGATTGCAGTCTCGTAACTCAATCCAACTTGAGAG10.6622516556291391No Hit
GAATTGAGTAGAGAGAACTCATGGGAGAAGAAAAGGTCTGTATCTGTCTCT10.6622516556291391No Hit
GCTTACTAGAAGACTGTGACCGCGGAATTCTGTCGGAATGTTTCCTTGTTT10.6622516556291391No Hit
AAGTTGTAGTAGCAATAAGATTTGTTTTATGTTGTGCTTTGTCATACAACA10.6622516556291391No Hit
GTAGCGCAATCTCCATTTAAGAATTTGATGATGATGGGTTTCATGATGTGG10.6622516556291391No Hit
CGCTTTCACATTGTCGATTGTGTCTGATGATTCTACCTCTAAGGTCTGTCT10.6622516556291391No Hit
ACATCGAGGATACCCCAGTACGCGGTTTGCCCTGGAGCCCCCAGAATGGCC10.6622516556291391No Hit
ACTTTCATTTGCTTAGAGAGATTCATGAACAAATTAGTTTCTGTCTCTTAT10.6622516556291391No Hit
CTCTAACTAAAGTAAAGAGAACTACTTTCTTGGTCATGTTACTTCCACACT10.6622516556291391No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers