##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23V3BCXX l02n01 kb2_4_29.3410000000aeb0.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3753 Sequences flagged as poor quality 0 Sequence length 51 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.61017852384759 38.0 38.0 38.0 32.0 38.0 2 36.5888622435385 38.0 38.0 38.0 32.0 38.0 3 36.59099387156941 38.0 38.0 38.0 32.0 38.0 4 36.735145217159605 38.0 38.0 38.0 32.0 38.0 5 36.78763655742073 38.0 38.0 38.0 32.0 38.0 6 38.7095656807887 40.0 38.0 40.0 38.0 40.0 7 38.41939781508127 40.0 38.0 40.0 38.0 40.0 8 38.429523048228084 40.0 38.0 40.0 38.0 40.0 9 38.541699973354646 40.0 38.0 40.0 38.0 40.0 10 38.41140420996536 40.0 38.0 40.0 38.0 40.0 11 38.48094857447376 40.0 38.0 40.0 38.0 40.0 12 38.40447641886491 40.0 38.0 40.0 38.0 40.0 13 38.42099653610445 40.0 38.0 40.0 38.0 40.0 14 38.507061017852386 40.0 38.0 40.0 38.0 40.0 15 38.52837729816147 40.0 38.0 40.0 38.0 40.0 16 38.40367705835332 40.0 38.0 40.0 38.0 40.0 17 38.38422595257128 40.0 38.0 40.0 38.0 40.0 18 38.31068478550493 40.0 38.0 40.0 38.0 40.0 19 38.48387956301625 40.0 38.0 40.0 38.0 40.0 20 38.39381827871036 40.0 38.0 40.0 38.0 40.0 21 38.49240607513989 40.0 38.0 40.0 38.0 40.0 22 38.34532374100719 40.0 38.0 40.0 38.0 40.0 23 38.39968025579537 40.0 38.0 40.0 38.0 40.0 24 38.35758060218492 40.0 38.0 40.0 38.0 40.0 25 38.32134292565947 40.0 38.0 40.0 38.0 40.0 26 38.20863309352518 40.0 38.0 40.0 38.0 40.0 27 38.117505995203835 40.0 38.0 40.0 38.0 40.0 28 38.082334132693845 40.0 38.0 40.0 32.0 40.0 29 37.92912336797229 40.0 38.0 40.0 32.0 40.0 30 37.87903010924594 40.0 38.0 40.0 32.0 40.0 31 37.807354116706634 40.0 38.0 40.0 32.0 40.0 32 37.58513189448441 40.0 38.0 40.0 32.0 40.0 33 37.383959499067416 40.0 38.0 40.0 32.0 40.0 34 37.21769251265654 40.0 38.0 40.0 32.0 40.0 35 37.03916866506795 40.0 38.0 40.0 32.0 40.0 36 36.80389022115641 40.0 38.0 40.0 27.0 40.0 37 36.615241140421 40.0 38.0 40.0 27.0 40.0 38 36.50786037836397 40.0 38.0 40.0 27.0 40.0 39 36.36237676525446 40.0 38.0 40.0 27.0 40.0 40 36.249933386624036 40.0 38.0 40.0 27.0 40.0 41 35.98721023181455 40.0 38.0 40.0 27.0 40.0 42 35.904876099120706 40.0 38.0 40.0 13.0 40.0 43 35.737276845190515 40.0 38.0 40.0 13.0 40.0 44 35.57100985877964 40.0 38.0 40.0 13.0 40.0 45 35.34132693844924 40.0 38.0 40.0 13.0 40.0 46 35.110844657607245 40.0 38.0 40.0 13.0 40.0 47 34.820676791899814 40.0 38.0 40.0 13.0 40.0 48 34.609912070343725 40.0 38.0 40.0 13.0 40.0 49 34.43991473487876 40.0 32.0 40.0 13.0 40.0 50 34.05462296829203 40.0 32.0 40.0 13.0 40.0 51 33.473754329869436 38.0 32.0 40.0 13.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2201 1 1.3215959821428598 2201 2 1.2646639384920704 2201 3 1.6722470238095255 2201 4 1.0414434523809462 2201 5 1.1233630952380977 2201 6 0.861991567460322 2201 7 1.1570498511904788 2201 8 1.3174417162698475 2201 9 0.9189422123015873 2201 10 1.4648313492063423 2201 11 0.25610119047618696 2201 12 -0.09372519841270588 2201 13 1.0875992063492106 2201 14 0.5733072916666586 2201 15 0.6096230158730265 2201 16 0.7208147321428768 2201 17 1.3924913194444457 2201 18 1.216214037698414 2201 19 0.9640004960317512 2201 20 1.1392733134920476 2201 21 0.940618799603179 2201 22 1.4701078869047635 2201 23 1.329935515873018 2201 24 1.429408482142847 2201 25 1.537090773809524 2201 26 1.051122271825406 2201 27 1.4841951884920732 2201 28 1.860584077380956 2201 29 1.5368675595237988 2201 30 1.6359437003968296 2201 31 1.991945684523813 2201 32 2.0181361607142847 2201 33 2.104284474206345 2201 34 2.6252666170634953 2201 35 2.3822048611111057 2201 36 2.700849454365084 2201 37 3.553193204365087 2201 38 1.6454179067460188 2201 39 3.4336433531745953 2201 40 1.8328311011904788 2201 41 3.3815476190476232 2201 42 3.8751674107142833 2201 43 4.148567708333331 2201 44 4.25015500992064 2201 45 3.901816716269849 2201 46 4.0369605654762 2201 47 4.725167410714292 2201 48 3.4531187996031747 2201 49 4.557440476190493 2201 50 4.19552331349206 2201 51 0.6299603174603092 1101 1 1.3215959821428598 1101 2 1.2646639384920704 1101 3 0.1722470238095255 1101 4 1.0414434523809462 1101 5 1.1233630952380977 1101 6 0.2619915674603206 1101 7 1.0570498511904773 1101 8 0.8174417162698475 1101 9 0.3189422123015859 1101 10 0.9648313492063423 1101 11 1.4561011904761898 1101 12 0.006274801587295542 1101 13 1.4875992063492092 1101 14 1.3733072916666558 1101 15 1.4096230158730236 1101 16 1.520814732142874 1101 17 0.8924913194444457 1101 18 0.6162140376984127 1101 19 -1.1359995039682502 1101 20 1.0392733134920462 1101 21 -0.6593812003968225 1101 22 0.9701078869047635 1101 23 1.2299355158730165 1101 24 0.929408482142847 1101 25 1.037090773809524 1101 26 0.45112227182540465 1101 27 0.9841951884920732 1101 28 -6.889415922619044 1101 29 -5.813132440476203 1101 30 -4.814056299603173 1101 31 -5.358054315476188 1101 32 -5.331863839285717 1101 33 -6.745715525793656 1101 34 -6.624733382936505 1101 35 -4.467795138888896 1101 36 -4.149150545634917 1101 37 -5.696806795634913 1101 38 -4.104582093253981 1101 39 0.08364335317459393 1101 40 0.08283110119047876 1101 41 2.781547619047622 1101 42 3.275167410714282 1101 43 3.54856770833333 1101 44 0.40015500992063835 1101 45 -3.048183283730154 1101 46 -2.413039434523803 1101 47 -1.6248325892857096 1101 48 2.2031187996031747 1101 49 -2.39255952380951 1101 50 -1.9544766865079382 1101 51 -2.420039682539688 2202 1 -1.0784040178571388 2202 2 1.2646639384920704 2202 3 -0.7277529761904731 2202 4 1.0414434523809462 2202 5 -0.0766369047619051 2202 6 0.46199156746032344 2202 7 0.3570498511904745 2202 8 0.9174417162698489 2202 9 -0.6810577876984141 2202 10 -2.335168650793655 2202 11 -0.5438988095238102 2202 12 1.106274801587297 2202 13 -0.11240079365079225 2202 14 0.5733072916666586 2202 15 -0.990376984126975 2202 16 0.32081473214287115 2202 17 0.5924913194444485 2202 18 -0.38378596230158735 2202 19 -0.6359995039682502 2202 20 0.7392733134920491 2202 21 0.5406187996031804 2202 22 0.6701078869047663 2202 23 -0.2700644841269835 2202 24 1.0294084821428484 2202 25 1.1370907738095255 2202 26 -0.5488777281745953 2202 27 0.6841951884920761 2202 28 1.060584077380959 2202 29 1.5368675595237988 2202 30 2.035943700396828 2202 31 2.3919456845238116 2202 32 2.4181361607142833 2202 33 2.5042844742063437 2202 34 1.8252666170634981 2202 35 1.9822048611111072 2202 36 2.700849454365084 2202 37 3.553193204365087 2202 38 3.2454179067460203 2202 39 3.0336433531745968 2202 40 3.0328311011904816 2202 41 3.3815476190476232 2202 42 2.275167410714282 2202 43 -1.2514322916666671 2202 44 -1.1498449900793588 2202 45 -1.4981832837301567 2202 46 -6.363039434523802 2202 47 -7.274832589285708 2202 48 -7.946881200396824 2202 49 -3.0425595238095084 2202 50 -2.0044766865079353 2202 51 -2.7700396825396894 1102 1 1.3215959821428598 1102 2 1.2646639384920704 1102 3 1.6722470238095255 1102 4 -0.45855654761905384 1102 5 -0.37663690476190226 1102 6 -0.2380084325396794 1102 7 0.5570498511904773 1102 8 0.3174417162698475 1102 9 -0.18105778769841407 1102 10 1.4648313492063423 1102 11 -0.0438988095238102 1102 12 0.5062748015872955 1102 13 0.9875992063492092 1102 14 0.8733072916666558 1102 15 0.40962301587302363 1102 16 1.020814732142874 1102 17 0.3924913194444457 1102 18 0.6162140376984127 1102 19 0.3640004960317498 1102 20 1.0392733134920462 1102 21 0.8406187996031775 1102 22 0.9701078869047635 1102 23 0.22993551587301653 1102 24 0.429408482142847 1102 25 -1.462909226190476 1102 26 0.45112227182540465 1102 27 -1.0158048115079268 1102 28 -2.389415922619044 1102 29 -0.5631324404762026 1102 30 0.9359437003968267 1102 31 0.8919456845238116 1102 32 0.9181361607142833 1102 33 1.0042844742063437 1102 34 1.6252666170634953 1102 35 1.2822048611111043 1102 36 2.1008494543650826 1102 37 -3.696806795634913 1102 38 2.645417906746019 1102 39 2.833643353174594 1102 40 2.3328311011904788 1102 41 -0.4684523809523782 1102 42 0.5251674107142819 1102 43 -3.20143229166667 1102 44 -2.5998449900793617 1102 45 0.9518167162698461 1102 46 -4.413039434523803 1102 47 -4.12483258928571 1102 48 1.2031187996031747 1102 49 -1.3925595238095099 1102 50 -1.4544766865079382 1102 51 -0.42003968253968793 2203 1 0.12159598214285694 2203 2 1.2646639384920704 2203 3 0.47224702380952266 2203 4 -0.1585565476190567 2203 5 1.1233630952380977 2203 6 1.2619915674603206 2203 7 -0.04295014880952408 2203 8 0.9174417162698489 2203 9 -0.2810577876984155 2203 10 0.6648313492063451 2203 11 1.0561011904761912 2203 12 1.106274801587297 2203 13 1.4875992063492092 2203 14 0.9733072916666572 2203 15 1.009623015873025 2203 16 1.1208147321428754 2203 17 1.3924913194444457 2203 18 1.216214037698414 2203 19 0.5640004960317526 2203 20 1.1392733134920476 2203 21 1.3406187996031775 2203 22 1.4701078869047635 2203 23 1.7299355158730165 2203 24 1.0294084821428484 2203 25 1.537090773809524 2203 26 1.4511222718254047 2203 27 1.4841951884920732 2203 28 1.4605840773809575 2203 29 1.1368675595238003 2203 30 2.035943700396828 2203 31 1.5919456845238145 2203 32 2.0181361607142847 2203 33 2.104284474206345 2203 34 2.2252666170634967 2203 35 2.3822048611111057 2203 36 3.1008494543650826 2203 37 3.553193204365087 2203 38 3.2454179067460203 2203 39 3.4336433531745953 2203 40 3.0328311011904816 2203 41 3.781547619047622 2203 42 4.275167410714282 2203 43 3.748567708333333 2203 44 3.850155009920641 2203 45 3.901816716269849 2203 46 4.436960565476198 2203 47 4.725167410714292 2203 48 4.6531187996031775 2203 49 5.35744047619049 2203 50 5.795523313492062 2203 51 6.4299603174603135 1103 1 -0.17840401785714022 1103 2 -0.23533606150792963 1103 3 0.1722470238095255 1103 4 -0.45855654761905384 1103 5 -0.37663690476190226 1103 6 0.7619915674603206 1103 7 0.5570498511904773 1103 8 0.8174417162698475 1103 9 0.8189422123015859 1103 10 0.9648313492063423 1103 11 0.9561011904761898 1103 12 0.5062748015872955 1103 13 0.9875992063492092 1103 14 0.37330729166665577 1103 15 0.40962301587302363 1103 16 1.020814732142874 1103 17 1.3924913194444457 1103 18 1.1162140376984127 1103 19 0.8640004960317498 1103 20 -5.210726686507954 1103 21 -1.9093812003968225 1103 22 -1.7798921130952365 1103 23 -1.5200644841269835 1103 24 -0.570591517857153 1103 25 1.037090773809524 1103 26 0.9511222718254047 1103 27 0.9841951884920732 1103 28 1.360584077380956 1103 29 1.4368675595237974 1103 30 1.9359437003968267 1103 31 1.8919456845238116 1103 32 1.4181361607142833 1103 33 1.0042844742063437 1103 34 -4.624733382936505 1103 35 -4.467795138888896 1103 36 -4.149150545634917 1103 37 -3.696806795634913 1103 38 -0.10458209325398116 1103 39 -3.416356646825406 1103 40 -4.417168898809521 1103 41 -3.968452380952378 1103 42 -2.974832589285718 1103 43 0.79856770833333 1103 44 0.9001550099206383 1103 45 -2.548183283730154 1103 46 1.836960565476197 1103 47 2.6251674107142904 1103 48 1.4531187996031747 1103 49 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35 -0.8177951388888971 2204 36 -4.6991505456349145 2204 37 0.15319320436508832 2204 38 -5.954582093253983 2204 39 2.633643353174591 2204 40 1.4328311011904802 2204 41 0.38154761904762324 2204 42 -1.924832589285721 2204 43 -4.051432291666671 2204 44 -3.7498449900793602 2204 45 -2.6981832837301525 2204 46 1.4369605654761983 2204 47 -2.8748325892857096 2204 48 -0.34688120039682246 2204 49 -5.842559523809509 2204 50 -2.204476686507938 2204 51 -1.5700396825396865 2205 1 -1.6784040178571402 2205 2 -3.5686693948412653 2205 3 0.6722470238095255 2205 4 -0.9585565476190538 2205 5 -1.8766369047619023 2205 6 -0.4046750992063437 2205 7 -0.44295014880952266 2205 8 -4.1825582837301525 2205 9 -0.014391121031749776 2205 10 -0.5351686507936577 2205 11 0.1227678571428541 2205 12 -1.6603918650793688 2205 13 -0.8457341269841265 2205 14 0.03997395833332007 2205 15 -0.9237103174603121 2205 16 -0.479185267857126 2205 17 -0.6075086805555543 2205 18 -1.217119295634923 2205 19 -3.8026661706349145 2205 20 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1212 11 -0.2581845238095255 1212 12 0.07770337301586494 1212 13 0.6304563492063551 1212 14 -1.4838355654761983 1212 15 -0.30466269841269167 1212 16 0.3779575892857281 1212 17 0.5353484623015916 1212 18 0.18764260912698205 1212 19 0.22114335317460387 1212 20 0.6821304563491921 1212 21 -0.37366691468253777 1212 22 1.1843936011904788 1212 23 0.8727926587301624 1212 24 1.1436941964285623 1212 25 0.10851934523809348 1212 26 0.8796937003968353 1212 27 0.6270523313492191 1212 28 0.1462983630952408 1212 29 1.0797247023809433 1212 30 1.0073722718253961 1212 31 0.963374255952381 1212 32 0.9895647321428527 1212 33 1.9328559027777743 1212 34 1.7681237599206412 1212 35 1.9250620039682502 1212 36 2.2437065972222285 1212 37 2.1246217757936563 1212 38 3.359703621031734 1212 39 -1.166356646825406 1212 40 2.404259672619048 1212 41 3.2101190476190524 1212 42 2.5608816964285666 1212 43 3.691424851190476 1212 44 3.7930121527777843 1212 45 2.987531001984131 1212 46 3.4083891369047663 1212 47 3.69659598214286 1212 48 2.16740451388889 1212 49 4.214583333333344 1212 50 3.795523313492062 1212 51 5.115674603174597 1213 1 -1.6784040178571402 1213 2 1.2646639384920704 1213 3 0.9222470238095255 1213 4 0.29144345238094616 1213 5 -2.0016369047619023 1213 6 0.7619915674603206 1213 7 -2.5679501488095227 1213 8 -1.8075582837301525 1213 9 -1.681057787698414 1213 10 0.7148313492063423 1213 11 0.4561011904761898 1213 12 -0.49372519841270446 1213 13 -0.012400793650790831 1213 14 0.12330729166665577 1213 15 0.15962301587302363 1213 16 -2.604185267857126 1213 17 -1.1075086805555543 1213 18 -2.6337859623015873 1213 19 -1.1359995039682502 1213 20 -0.2107266865079538 1213 21 -0.40938120039682246 1213 22 -0.27989211309523654 1213 23 -2.3950644841269835 1213 24 -0.945591517857153 1213 25 0.2870907738095241 1213 26 -0.04887772817459535 1213 27 0.7341951884920732 1213 28 -0.1394159226190439 1213 29 0.6868675595237974 1213 30 0.4359437003968267 1213 31 0.8919456845238116 1213 32 -1.7068638392857167 1213 33 0.7542844742063437 1213 34 1.1252666170634953 1213 35 -1.0927951388888957 1213 36 0.6008494543650826 1213 37 -1.821806795634913 1213 38 1.0204179067460188 1213 39 0.4586433531745939 1213 40 1.8328311011904788 1213 41 1.7815476190476218 1213 42 2.025167410714282 1213 43 2.54856770833333 1213 44 3.1501550099206383 1213 45 2.701816716269846 1213 46 2.836960565476197 1213 47 0.2501674107142904 1213 48 2.9531187996031747 1213 49 3.60744047619049 1213 50 3.170523313492062 1213 51 -3.795039682539688 >>END_MODULE >>Per sequence quality scores pass #Quality Count 22 3.0 23 8.0 24 2.0 25 12.0 26 28.0 27 43.0 28 55.0 29 62.0 30 97.0 31 104.0 32 104.0 33 127.0 34 179.0 35 160.0 36 205.0 37 275.0 38 497.0 39 1792.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.45619658119658 15.892094017094017 12.793803418803417 27.857905982905983 2 12.54996003197442 22.675193178790302 47.108979483080205 17.665867306155075 3 17.559285904609645 28.457234212629896 38.55582200905942 15.42765787370104 4 9.645616839861445 25.33972821742606 36.79722888355982 28.21742605915268 5 12.576605382360778 33.54649613642419 39.7015720756728 14.175326405542233 6 22.70183852917666 42.7124966693312 18.012256861177725 16.573407940314418 7 21.58273381294964 30.349054090061284 23.92752464694911 24.14068745003997 8 22.062350119904075 38.15614175326406 21.849187316813214 17.932320810018652 9 21.875832667199575 14.841460165201173 23.501199040767386 39.781508126831866 10 16.147082334132694 33.65307753796962 28.75033306687983 21.449507061017854 11 24.64694910738076 22.968292033040235 35.225153210764724 17.15960564881428 12 21.103117505995204 24.2472688515854 31.361577404742874 23.288036237676526 13 25.1265654143352 19.211297628563813 38.44924060751399 17.212896349586998 14 17.292832400746068 18.998134825472953 33.89288569144684 29.816147082334133 15 17.292832400746068 24.300559552358113 29.682920330402347 28.723687716493473 16 25.73940847322142 25.393018918198774 30.16253663735678 18.705035971223023 17 16.759925393018918 33.89288569144684 28.883559818811616 20.46362909672262 18 28.670397015720756 23.527844391153742 31.49480415667466 16.30695443645084 19 22.195576871835865 21.68931521449507 31.361577404742874 24.75353050892619 20 18.73168132160938 20.303756994404477 32.001065814015455 28.96349586997069 21 17.719158006927792 22.168931521449505 32.74713562483347 27.364774846789235 22 16.653343991473488 34.05275779376499 30.988542499333864 18.305355715427655 23 16.17372768451905 26.378896882494008 31.60138555822009 25.845989874766857 24 26.64535038635758 23.980815347721823 33.306687982946976 16.06714628297362 25 16.573407940314418 34.13269384492406 32.880362376765255 16.41353583799627 26 25.73940847322142 27.28483879563016 33.20010658140154 13.775646149746871 27 16.253663735678124 37.0636823874234 31.974420463629095 14.708233413269383 28 14.388489208633093 26.991739941380228 31.654676258992804 26.96509459099387 29 13.962163602451373 36.104449773514524 30.988542499333864 18.94484412470024 30 23.634425792699172 26.96509459099387 31.761257660538234 17.63922195576872 31 26.458832933653078 26.192379429789504 31.734612310151878 15.61417532640554 32 16.999733546496138 36.077804423128164 30.90860644817479 16.013855582200907 33 14.761524114042098 28.457234212629896 40.47428723687717 16.30695443645084 34 24.966693312017053 26.911803890221158 31.894484412470025 16.227018385291768 35 16.466826538768984 26.96509459099387 40.50093258726352 16.06714628297362 36 25.366373567812417 27.311484146016518 29.97601918465228 17.346123101518785 37 16.20037303490541 37.410071942446045 30.96189714894751 15.42765787370104 38 14.228617106314948 29.54969357847056 38.76898481215028 17.452704503064215 39 13.056221689315214 28.11084465760725 31.761257660538234 27.0716759925393 40 12.789768185451639 28.350652811084466 40.92725819344524 17.932320810018652 41 13.908872901678656 27.63122835065281 29.73621103117506 28.723687716493473 42 23.26139088729017 27.33812949640288 28.883559818811616 20.516919797495337 43 15.134559019451105 27.81774580335731 36.82387423394617 20.223820943245403 44 13.056221689315214 37.49000799360512 28.377298161470822 21.076472155608847 45 12.86970423661071 28.084199307220892 36.82387423394617 22.22222222222222 46 22.675193178790302 26.778577138289368 28.697042366107112 21.849187316813214 47 14.761524114042098 25.153210764721557 26.885158539834798 33.20010658140154 48 13.53583799626965 25.179856115107913 27.0716759925393 34.212629896083136 49 22.70183852917666 24.46043165467626 26.352251532107644 26.485478284039438 50 14.521715960564881 23.71436184385825 35.891286970423664 25.87263522515321 51 13.695710098587796 25.01998401278977 25.23314681588063 36.051159072741804 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 2.0 10 4.0 11 8.0 12 12.0 13 27.0 14 42.0 15 53.0 16 64.0 17 75.0 18 86.0 19 84.0 20 82.0 21 93.0 22 104.0 23 114.5 24 125.0 25 129.0 26 111.0 27 89.0 28 88.0 29 87.0 30 107.5 31 128.0 32 165.5 33 203.0 34 206.0 35 209.0 36 208.0 37 207.0 38 198.0 39 189.0 40 177.0 41 165.0 42 148.5 43 132.0 44 148.0 45 164.0 46 164.5 47 165.0 48 165.5 49 166.0 50 200.0 51 234.0 52 401.5 53 569.0 54 345.5 55 122.0 56 105.5 57 89.0 58 73.5 59 58.0 60 49.5 61 41.0 62 31.5 63 22.0 64 20.5 65 19.0 66 15.5 67 12.0 68 13.0 69 14.0 70 10.5 71 7.0 72 4.5 73 2.0 74 3.0 75 4.0 76 4.0 77 2.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2398081534772182 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 3753.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.69624300559552 #Duplication Level Percentage of deduplicated Percentage of total 1 95.62019391507857 76.20570210498268 2 2.5743898361751922 4.103383959499068 3 0.8358408559010365 1.9984012789768184 4 0.26746907388833163 0.8526512123634425 5 0.13373453694416582 0.5329070077271516 6 0.033433634236041454 0.15987210231814547 7 0.10030090270812438 0.5595523581135092 8 0.0 0.0 9 0.06686726847208291 0.4796163069544364 >10 0.3343363423604146 5.1958433253397285 >50 0.0 0.0 >100 0.033433634236041454 9.91207034372502 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC 372 9.91207034372502 No Hit TCTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGT 48 1.2789768185451638 No Hit GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 23 0.6128430588862244 No Hit GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA 20 0.5329070077271516 No Hit CTCTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCG 18 0.4796163069544364 No Hit GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 18 0.4796163069544364 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16 0.4263256061817213 No Hit TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA 15 0.3996802557953637 No Hit GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 14 0.37303490540900613 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13 0.3463895550226485 No Hit TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA 10 0.2664535038635758 No Hit GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA 9 0.2398081534772182 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9 0.2398081534772182 No Hit GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7 0.18651745270450307 No Hit GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGA 7 0.18651745270450307 No Hit GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA 7 0.18651745270450307 No Hit GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG 6 0.15987210231814547 No Hit CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC 5 0.1332267519317879 RNA PCR Primer, Index 23 (95% over 21bp) GCGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCT 5 0.1332267519317879 No Hit GGTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA 5 0.1332267519317879 No Hit GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA 5 0.1332267519317879 No Hit GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA 4 0.10658140154543032 No Hit GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4 0.10658140154543032 No Hit GAGTACGGGGAATGCATTGGATGGATGCCCGGGCATTGAGAAGGAAGGACG 4 0.10658140154543032 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT 4 0.10658140154543032 No Hit GGCATTGAGAAGGAAGGACGCTTTCAGAGGCGAAAGGCCATGGGGAGATAC 4 0.10658140154543032 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT 4 0.10658140154543032 No Hit GTATCAACGCAGAGTACGGGCAGCTGTCTCTTATACACATCTCCGAGCCCA 4 0.10658140154543032 No Hit GTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4 0.10658140154543032 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.02664535038635758 9 0.0 0.0 0.02664535038635758 10 0.0 0.0 0.02664535038635758 11 0.0 0.0 0.02664535038635758 12 0.0 0.0 0.02664535038635758 13 0.0 0.0 0.02664535038635758 14 0.0 0.0 0.02664535038635758 15 0.0 0.0 0.02664535038635758 16 0.0 0.0 0.02664535038635758 17 0.0 0.0 0.02664535038635758 18 0.0 0.0 0.05329070077271516 19 0.0 0.0 0.05329070077271516 20 0.0 0.0 0.10658140154543032 21 0.0 0.0 0.21316280309086064 22 0.0 0.0 0.3996802557953637 23 0.0 0.0 0.826005861977085 24 0.0 0.0 1.4388489208633093 25 0.0 0.0 1.7319477751132428 26 0.0 0.0 1.9184652278177459 27 0.0 0.0 2.104982680522249 28 0.0 0.0 2.2648547828403944 29 0.0 0.0 2.9576338928856916 30 0.0 0.0 3.9168665067945643 31 0.0 0.0 4.662936317612576 32 0.0 0.0 5.595523581135092 33 0.0 0.0 6.261657340794032 34 0.0 0.0 7.194244604316546 35 0.0 0.0 7.887023714361844 36 0.0 0.0 8.739674926725286 37 0.0 0.0 9.645616839861445 38 0.0 0.0 10.89794830802025 39 0.0 0.0 12.176925126565415 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCCGTC 35 8.385177E-8 44.986664 45 GGATGTG 45 6.044156E-7 34.989628 30 AGCCCAC 45 6.044156E-7 34.989628 17 GTATGCC 45 6.044156E-7 34.989628 42 ATCTCGT 45 6.044156E-7 34.989628 37 GCCCACG 45 6.044156E-7 34.989628 18 GACAGGA 45 6.044156E-7 34.989628 26 AGGATGT 45 6.044156E-7 34.989628 29 CCACGAG 45 6.044156E-7 34.989628 20 TGATCTC 45 6.044156E-7 34.989628 35 TATGCCG 45 6.044156E-7 34.989628 43 GATGTGA 45 6.044156E-7 34.989628 31 GAGCCCA 45 6.044156E-7 34.989628 16 CACGAGA 45 6.044156E-7 34.989628 21 CCCACGA 45 6.044156E-7 34.989628 19 ATGTGAT 45 6.044156E-7 34.989628 32 TCTCGTA 45 6.044156E-7 34.989628 38 ACGAGAC 45 6.044156E-7 34.989628 22 GATCTCG 45 6.044156E-7 34.989628 36 ATGCCGT 45 6.044156E-7 34.989628 44 >>END_MODULE